远志地上部分10个黄酮类成分的分离及抗氧化活性研究

doi:10.5246/jcps.2013.01.004

远志地上部分10个黄酮类成分的分离及抗氧化活性研究

史天星, 李宇光, 姜勇^*, 屠鹏飞

1. 北京大学医学部药学院天然药物及仿生药物国家重点实验室, 北京 100191
2. 辽宁省辽河油田总医院神经外科, 辽宁盘锦 124010

收稿日期:2012-08-19 修回日期:2012-11-10 出版日期:2013-01-20 发布日期:2013-01-20
通讯作者: 姜勇*

Isolation of flavonoids from the aerial parts of Polygala tenuifolia Willd. and their antioxidant activities

Tianxing Shi, Yuguang Li, Yong Jiang^*, Pengfei Tu

1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
2. Neurosurgery, General Hospital of Liaohe Oilfield, Panjin 124010, China

Received:2012-08-19 Revised:2012-11-10 Online:2013-01-20 Published:2013-01-20
Contact: Yong Jiang*

摘要/Abstract

摘要：

研究远志地上部分的化学成分并测试其抗氧化活性。运用硅胶、Sephadex LH-20、ODS及半制备液相, 从远志地上部分中分离得到10个黄酮类化合物。用DPPH自由基清除试验测定了其抗氧化活性。分离所得化合物经光谱数据分析鉴定其结构分别为: 异鼠李素-3-O-β-D-吡喃葡萄糖苷 (1), 异鼠李素-3-O-β-D-吡喃半乳糖苷 (2), 槲皮素-3-O-β-D-吡喃葡萄糖(1→2)-β-D-吡喃半乳糖苷 (3), 槲皮素-3-O-β-D-吡喃葡萄糖(1→2)-β-D-吡喃葡萄糖苷 (4), 蒙花苷 (5), 槲皮素-3-O-β-D-吡喃葡萄糖苷 (6), 5,7-二羟基-3-甲氧基黄酮-7-O-β-D-葡萄糖醛酸 (7), 异鼠李素 (8), 山奈酚 (9)和槲皮素 (10)。所有化合物均为首次从该植物中分离得到, 化合物1-5和7均为首次从该属植物中分离得到。活性测试结果表明, 化合物3、4、6、8、9和10表现出一定的抗氧化活性。

关键词: 远志属, 远志, 地上部分, 黄酮, 抗氧化

Abstract:

The aim of this study was to isolate the chemical constituents of the aerial parts of Polygala tenuifolia Willd. and to determine their antioxidant activities. Ten flavonoids were isolated and purified by silica gel, Sephadex LH-20, and ODS column chromatography, and semi-preparative HPLC. Their structures were elucidated by spectroscopic analysis and identified as isorhamnetin-3-O-β-D-glucopyranoside (1), isorhamnetin-3-O-β-D-galactopyranoside (2), quercetin-3-O-β-D-glucopyranosyl (1→2)-β-D-galactopyranoside (3), quercetin-3-O-β-D-glucopyranosyl (1→2)-β-D-glucopyranoside (4), linarin (5), quercetin-3-O-β-D-glucopyranoside (6), 5,7-dihydroxy-8-methxoyflavone-7-O-β-D-glucuronoside (7), isorhamnetin (8), kaempferol (9) and quercetin (10). All these compounds were isolated from this plant for the first time, and compounds 1-5 and 7 were isolated from the genus of Polygala for the first time. The antioxidant activities of the isolated compounds were evaluated by DPPH free radical scavenging assay, and compounds 3, 4, 6, 8, 9 and 10 showed potent antioxidant activities.

Key words: Polygala, Polygala tenuifolia, Aerial parts, Flavonoids, Antioxidant activity

中图分类号:

R284.2

Supporting:

Foundation items: New-Century Talent Program, Ministry of Education of China (Grant No. 985-2-102-113), National Science Fund for Excellent Young Scholars (Grant No. 81222051), and National Key Technology R&D Program “New Drug Innovation” of China (Grant No. 2012ZX09304-005, 2012ZX09301002-002).
^*Corresponding author. Tel.: 86-10-82802719

史天星, 李宇光, 姜勇^*, 屠鹏飞. 远志地上部分10个黄酮类成分的分离及抗氧化活性研究[J]. , DOI: 10.5246/jcps.2013.01.004.

Tianxing Shi, Yuguang Li, Yong Jiang^*, Pengfei Tu. Isolation of flavonoids from the aerial parts of Polygala tenuifolia Willd. and their antioxidant activities[J]. , DOI: 10.5246/jcps.2013.01.004.

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