西藏麻黄乙醇提取物及其不同极性组分的体外抗氧化活性研究

doi:10.5246/jcps.2023.06.041

中国药学(英文版) ›› 2023, Vol. 32 ›› Issue (6): 478-486.DOI: 10.5246/jcps.2023.06.041

西藏麻黄乙醇提取物及其不同极性组分的体外抗氧化活性研究

王涵¹^,^#, 李芳龙¹^,^#, 阿呷尔布²^,^#, 梁晓霞¹^,^*(), 更桑³, 才让南迦³

1. 四川农业大学动物医学院天然药物研究中心, 四川成都 611130
2. 西藏大学医学院, 西藏拉萨 850000
3. 西藏藏医药大学, 西藏拉萨 850000

收稿日期:2022-11-29 修回日期:2022-12-09 接受日期:2023-01-17 出版日期:2023-07-01 发布日期:2023-07-01
通讯作者: 梁晓霞
作者简介:
+ Tel.: +86-18181996903, E-mail: liangxiaoxia@sicau.edu.cn
基金资助:
The National Natural Science Foundation of China (Grant No. 82260823), the co-construction and Innovation Fund for Tibet Economic and Social Development and Plateau Scientific Research (Grant No. 1zj2021014), and the 2020 Tibetan Medicine Doctoral Program of Tibet Medical University (Grant No. BSDJS-20-12).

The in vitro antioxidant activities of the ethanol extract and its different polar fractions from Ephedra saxatilis Royle ex Florin in Tibet

Han Wang¹^,^#, Fanglong Li¹^,^#, Aga Er-bu²^,^#, Xiaoxia Liang¹^,^*(), Geng Sang³, Car Rangnanjia³

1 Natural Medicine Research Center, College of veterinary medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
2 Medical college, Tibet University, Lasa 850000, Tibet, China
3 Tibet Medical University, Lasa 850000, Tibet, China

Received:2022-11-29 Revised:2022-12-09 Accepted:2023-01-17 Online:2023-07-01 Published:2023-07-01
Contact: Xiaoxia Liang
About author:
# Han Wang, Fanglong Li and Aga Er-bu contributed equally to this work.

摘要/Abstract

摘要：

本文对西藏麻黄乙醇提取物及其不同极性提取物的体外抗氧化活性进行了研究。首先采用化学显色方法对大类化学成分进行鉴别, 然后分别采用Folin-Ciocalteu法和氯化铝比色法对总多酚和总黄酮的含量进行鉴定, 再采用DPPH法、ABTS法、羟自由基清除和还原粉末法对其抗氧化活性进行了研究。麻黄中乙酸乙酯部分和二氯甲烷部分的抗氧化活性均优于BHT, 这与麻黄中多酚和黄酮含量较高有关。

关键词: 藏麻黄, 乙醇提取物, 不同极性部位, 抗氧化活性, 成分

Abstract:

In the present study, we aimed to investigate the in vitro antioxidant activity of the ethanol extract and its polar extracts from Ephedra saxatilis Royle ex Florin in Tibet. The chemical constituents were first screened by the preliminary phytochemical methods. The contents of total polyphenols and total flavonoids were identified by the Folin-Ciocalteu method and colorimetric method of the aluminum chloride method, respectively. Furthermore, their antioxidant activities were investigated by the DPPH method, ABTS method, hydroxyl radical scavenging, and reduction powder method. The preliminary phytochemical screening showed that the polyphenols, glycosides, flavonoids, and saponins existed in ethanol extract, and the chemical composition in different polar extracts varied, among which the contents of polyphenols and flavonoids were highest in the ethyl acetate part, followed by the dichloromethane part, exhibiting best antioxidant capacity. Both the ethyl acetate and dichloromethane parts from Ephedra saxatilis Royle ex Florin exhibited better antioxidant activities than BHT, which was associated with the high contents of polyphenols and flavonoids.

Key words: Ephedra saxatilis Royle ex Florin, Ethanol extract, Different polar parts, Antioxidants, Constituents

Supporting:

王涵, 李芳龙, 阿呷尔布, 梁晓霞, 更桑, 才让南迦. 西藏麻黄乙醇提取物及其不同极性组分的体外抗氧化活性研究[J]. 中国药学(英文版), 2023, 32(6): 478-486.

Han Wang, Fanglong Li, Aga Er-bu, Xiaoxia Liang, Geng Sang, Car Rangnanjia. The in vitro antioxidant activities of the ethanol extract and its different polar fractions from Ephedra saxatilis Royle ex Florin in Tibet[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(6): 478-486.

图/表 6

Table 1. The consequence of preliminary phytochemical screening.

Figure 1. The standard curve of gallic acid.

Table 2. The consequence of polyphenol content determination.

Figure 2. The standard curve of Rutin.

Table 3. The consequence of flavonoid content determination.

Figure 3. The scavenging abilities against DPPH (a), ABTS (b), hydroxyl radical (c), and the total reducing power absorbance (d).

参考文献 23

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西藏麻黄乙醇提取物及其不同极性组分的体外抗氧化活性研究

The in vitro antioxidant activities of the ethanol extract and its different polar fractions from Ephedra saxatilis Royle ex Florin in Tibet

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