云南美登木愈伤组织化学成分的研究

doi:10.5246/jcps.2023.04.025

中国药学(英文版) ›› 2023, Vol. 32 ›› Issue (4): 283-290.DOI: 10.5246/jcps.2023.04.025

云南美登木愈伤组织化学成分的研究

蒋蕾, 涂渝娇, 代伟华, 袁琳^*()

昆明学院化学化工学院, 云南昆明 650214

收稿日期:2022-12-15 修回日期:2023-01-23 接受日期:2023-02-22 出版日期:2023-04-29 发布日期:2023-04-29
通讯作者: 袁琳
作者简介:
+ Tel.: +86-871-65098621, E-mail: sunrainyl@126.com
基金资助:
Yunnan Province Professional Degree Postgraduate Teaching Case Database Construction Project; the Science and Technology Planning Project of Yunnan Science and Technology Department Joint Project of Local Colleges and Universities (Grant No. 202001BA070001-218).

Chemical constituents from the calli of Maytenus hookeri

Lei Jiang, Yujiao Tu, Weihua Dai, Lin Yuan^*()

School of Chemistry and Chemical Engineering, Kunming University, Kunming 650214, China

Received:2022-12-15 Revised:2023-01-23 Accepted:2023-02-22 Online:2023-04-29 Published:2023-04-29
Contact: Lin Yuan

摘要/Abstract

摘要：

采用RP-18中压柱、硅胶柱和Sephadex LH-20柱等色谱技术对云南美登木愈伤组织的干燥细胞和新鲜细胞提取物的乙酸乙酯部分进行分离纯化得到16个化合物。通过核磁共振和质谱等波谱技术鉴定化学结构为雷公藤内酯甲 (1), 雷公藤内酯乙 (2), 3β,22α-二羟基齐墩果-12-烯-29-酸 (3), 22α-羟基-3-氧-齐墩果-12-烯-29-酸 (4), 3-表卡通酸 (5), 齐墩果-9(11),12-二烯-3-β-醇 (6), 22α-羟基-3-氧-乌苏-12-烯-30-酸 (7), 雷公藤三萜酸C (8), 2α,3β-二羟基-木栓烷-29-酸 (9), 美登酮酸 (10), 3β-羟基豆甾-5-烯-7-酮 (11), 7β-羟基谷甾醇 (12), 豆甾-4-烯-3-酮 (13), β-谷甾醇 (14), 1-β-D-glucpotyransyl-2,6-dimetoxi-4-propenyl-benzen (15)和腺苷 (16)。除化合物10和14外, 其余化合物均为首次从云南美登木植物细胞及其植株中分离得到。

关键词: 云南美登木, 愈伤组织, 化学成分, 柱层析

Abstract:

In order to study the chemical compounds of the calli induced from Maytenus hookeri, the dried and fresh cells of the calli were extracted by heat refluxing and cold soaking method, respectively. The chemical constituents were purified through various column chromatography including silica gel, RP-18 and Sephadex LH-20 from the ethyl acetate extract. According to the NMR and MS analysis, sixteen compounds were identified as wilforlide A (1), wilforlide B (2), 3β,22α-dihydroxyolean-12-en-29-oic acid (3), 22α-hydroxy-3-oxoolean-12-en-29-oic acid (4), 3-epikatonic acid (5), oleana-9(11),12-dien-3-β-ol (6), 22α-hydroxy-3-oxo-12-ursen-30-oic acid (7), triptotriterpenic acid C (8), 2α,3β-dihydroxy-29-friedelanoic acid (9), maytenonic acid (10), 3β-hydroxystigmast-5-en-7-one (11), 7β-hydroxysitosterol (12), stigmast-4-ene-3-one (13), β-sitosterol (14), 1-β-D-glucpotyransyl-2,6-dimetoxi-4-propenyl-benzene (15) and adenosine (16). All compounds were reported in the calli of M. hookeri and the plant for the first time except 10 and 14.

Key words: Maytenus hookeri, Calli, Chemical constituents, Column chromatography

Supporting:

蒋蕾, 涂渝娇, 代伟华, 袁琳. 云南美登木愈伤组织化学成分的研究[J]. 中国药学(英文版), 2023, 32(4): 283-290.

Lei Jiang, Yujiao Tu, Weihua Dai, Lin Yuan. Chemical constituents from the calli of Maytenus hookeri[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(4): 283-290.

图/表 1

参考文献 21

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云南美登木愈伤组织化学成分的研究

Chemical constituents from the calli of Maytenus hookeri

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