牡丹皮炭化学成分及其潜在凝血活性研究

doi:10.5246/jcps.2018.09.062

中国药学(英文版) ›› 2018, Vol. 27 ›› Issue (9): 608-616.DOI: 10.5246/jcps.2018.09.062

牡丹皮炭化学成分及其潜在凝血活性研究

杨小露^1,2,3#, 薛兴阳^4#, 林燕^1#, 黄绮韵¹, 莫毛燕¹, 王淑美^1,2,3*, 孟江^1,2,3*

1. 广东药科大学中药学院, 广东广州 510006
2. 国家中医药管理局中药数字化质量评估重点单位, 广东广州 510006
3. 广东省高校中医药质量工程技术研究中心, 广东广州 510006
4. 广州医科大学附属肿瘤医院, 广东广州 510006

收稿日期:2018-06-17 修回日期:2018-07-13 出版日期:2018-09-29 发布日期:2018-07-25
通讯作者: Tel./Fax: +86-020-39352169; +86-020-39352559, E-mail: jiangmeng666@126.com; 2395903468@qq.com
基金资助:
National Natural Science Foundation of China (Grant No. 81473352), Guangdong Province Innovation Training Project (Grant No. 201610573040), Guangzhou Science and Technology Bureau Foundation (Grant No. 201707010170).

Chemical constituents from the Moutan Cortex charcoal and their potential coagulation activities

Xiaolu Yang^1,2,3#, Xingyang Xue^4#, Yan Lin^1#, Qiyun Huang¹, Maoyan Mo¹, Shumei Wang^1,2,3*, Jiang Meng^1,2,3*

1. Department of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China
2. The Key Unit of Chinese Medicine Digitalization Quality Evaluation of State Administration of Traditional Chinese Medicine, Guangzhou 510006, China
3. The Research Center for Quality Engineering Technology of Traditional Chinese Medicine at Guangdong Universities, Guangzhou 510006, China
4. Guangzhou Medical University Cancer Hospital and Institute, Guangzhou 510006, China

Received:2018-06-17 Revised:2018-07-13 Online:2018-09-29 Published:2018-07-25
Contact: Tel./Fax: +86-020-39352169; +86-020-39352559, E-mail: jiangmeng666@126.com; 2395903468@qq.com
Supported by:
National Natural Science Foundation of China (Grant No. 81473352), Guangdong Province Innovation Training Project (Grant No. 201610573040), Guangzhou Science and Technology Bureau Foundation (Grant No. 201707010170).

摘要/Abstract

摘要：

对中药牡丹皮炭的化学成分进行深入研究, 并对其凝血活性进行筛选。通过聚酰胺、硅胶、葡聚糖凝胶等柱色谱方法进行分离纯化, 利用核磁共振波谱等方法鉴定结构。共分离鉴定了10个化合物, 分别为3-(2-呋喃)-1-(2-羟基-4-甲氧基苯基)-2-丙烯酮 (1)、2-(2,5-羟基-4-甲基苯基)丙酸乙酯 (2)、5-十四烯酸甲酯 (3)、1,4-二乙基-环己烷(4)、儿茶酚 (5)、对羟基苯甲酸甲酯 (6)、3-羟基-2-甲基-4-吡喃酮 (7)、3,8-二羟基-2-甲基色酮 (8)、3β-羟基-齐墩果烷-12-烯 (9)、1-亚油酸单甘油酯 (10)。其中1, 2为新天然产物, 3–10为首次从牡丹皮炭中分离得到。在体外评估化合物潜在的凝血活性, 化合物2, 9和10具有强烈的止血作用, 而化合物1和8可以活化血液循环。

关键词: 牡丹皮炭, 化学成分, 凝血活性

Abstract:

The chemical constituents of Cortex Moutan charcoal were studied in depth, and their coagulation activity was screened. The chemical constituents were isolated by polyamide, silica gel and Sephadex G-Sepharose chromatography purification,and their structures were identified by NMR spectroscopy and other analyses. A total of 10 compounds were obtained and identified as follows: 3-(2-furan)-1-(2-hydroxy-4-methoxy-phenyl)-2-propylene ketone (1), 2-(2,5-hydroxy-4-methyl phenyl) propionate ethyl ester (2), methyl tetradec-5-enoate (3), 1,4-diethylcyclohexane (4), catechol (5), methyl-4-hydroxybenzoate (6), 3-hydroxy-2-methyl-4-pyrone (7), 3,8-dihydroxy-2-metyl-chromone (8), 3β-hydroxy-olean-12-en (9), 1-monolinolein (10). Among them, compounds 1 and 2 were new natural products, and 3–10 were isolated from the Cortex Moutan charcoal for the first time. The potential coagulation activities of compounds were evaluated, and compounds 2, 9 and 10had strong hemostatic effects. While compounds 1 and 8 could significantly activate blood circulation.

Key words: Moutan Cortex charcoal, Chemical constituents, Coagulation activities

中图分类号:

R284

Supporting:

杨小露, 薛兴阳, 林燕, 黄绮韵, 莫毛燕, 王淑美, 孟江. 牡丹皮炭化学成分及其潜在凝血活性研究[J]. 中国药学(英文版), 2018, 27(9): 608-616.

Xiaolu Yang, Xingyang Xue, Yan Lin, Qiyun Huang, Maoyan Mo, Shumei Wang, Jiang Meng. Chemical constituents from the Moutan Cortex charcoal and their potential coagulation activities[J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(9): 608-616.

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牡丹皮炭化学成分及其潜在凝血活性研究

Chemical constituents from the Moutan Cortex charcoal and their potential coagulation activities

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