千金子制霜前后UPLC-Q-TOF/MS化学成分分析研究

doi:10.5246/jcps.2020.07.044

中国药学(英文版) ›› 2020, Vol. 29 ›› Issue (7): 470-479.DOI: 10.5246/jcps.2020.07.044

千金子制霜前后UPLC-Q-TOF/MS化学成分分析研究

Huinan Wang¹, Jingzhen Zhang¹, Yuexin Cui¹, Siyu Wang¹, Hui Gao¹, Yao Zhang¹, Xinjie Wang¹, Ziye Yang¹, Mengyu Chen¹, Peihua Wang¹, Guimei Zhang¹, Yingzi Wang^1*, Chao Zhang^2*

1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
2. School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, China

收稿日期:2020-03-28 修回日期:2020-04-16 出版日期:2020-07-31 发布日期:2020-05-18
通讯作者: Tel.: +86-10-84738615, E-mail: wangyzi@sina.com; zhangchaotcm@126.com
基金资助:
Beijing Natural Science Foundation (Grant No. 7182097), National Natural Science foundation of China (Grant No. 81673597) and National Key Research and Development Program of China (Grant No. 2018YFE0197900).

Chemical comparison of Semen Euphorbiae and Semen Euphorbiae Pulveratum by UPLC-Q-TOF/MS coupled with multivariate statistical techniques

王慧楠¹, 张景珍¹, 崔曰新¹, 王思雨¹, 高慧¹, 张瑶¹, 王新杰¹, 杨子烨¹, 陈梦雨¹, 王佩华¹, 张桂梅¹, 王英姿^1*, 张超^2*

1. 北京中医药大学中药学院, 北京 102488
2. 山东中医药大学药学院, 山东济南 250355

Received:2020-03-28 Revised:2020-04-16 Online:2020-07-31 Published:2020-05-18
Contact: Tel.: +86-10-84738615, E-mail: wangyzi@sina.com; zhangchaotcm@126.com
Supported by:
Beijing Natural Science Foundation (Grant No. 7182097), National Natural Science foundation of China (Grant No. 81673597) and National Key Research and Development Program of China (Grant No. 2018YFE0197900).

摘要/Abstract

摘要：

本文建立了一种超高效液相色谱-飞行时间-质谱联用技术(UPLC-Q-TOF/MS)全面快速地分析千金子制霜前后的化学成分变化的方法,为进一步阐明千金子制霜减毒机理提供参考。采用UPLC-Q-TOF/MS技术分析了千金子制霜前后成分变化,并借助SIMCA-P软件,基于主成分分析法(PCA)和偏最小二乘判别法(PLS-DA)多变量统计方法分析千金子制霜前后化学成分量的变化。结果发现,千金子制霜前后化学成分差异显著,从中推断出14个潜在的化学标记物,正离子模式下,千金子制霜后千金子素L₂、L₃、L_7a、L₈、L₉和续随子醇的量明显下降;负离子模式下,霜品中秦皮乙素、双七叶内酯、巨大戟醇和棕榈酸的量明显升高,千金子素L₁、L₄和L₅的量明显降低。UPLC-Q-TOF/MS技术结合多变量统计方法研究千金子制霜前后化学成分复杂变化具有一定的优势,为阐明其制霜减毒机理提供了重要科学依据。

关键词: 千金子, 千金子霜, UPLC-Q-TOF/MS, 多变量统计, 化学成分, 炮制机理

Abstract:

In the present study, we aimed to assess the chemical composition changes of Semen Euphorbiae (SE) and Semen Euphorbiae Pulveratum (SEP) by UPLC-Q-TOF/MS and multivariate statistical methods. The UPLC-Q-TOF/MS method and SIMCA-P software were used to analyze the changes of chemical components of SE and SEP based on PCA and PLS-DA multivariate statistical methods. A “component-target-disease” network model was constructed by Intelligent Platform for Life Sciences of traditional Chinese medicine (TCM) to predict potential related diseases. The differences of chemical composition were significant between SE and SEP. Under positive ion mode, the amounts of Euphorbia factor L₂, L₃, L_7a, L₈, L₉ and lathyrol were obviously decreased after processing. Under negative ion mode, the amounts of aesculetin, bisaesculetin, ingenol and cetylic acid were increasedobviously, while Euphorbia factor L₁, L₄ and L₅ were decreased obviously after processing, and the components of euphobiasteroid, aesculetin, lathyrol and linoleic acid among the 14 differentiated compounds were closely related to the SE-related diseases through the “component-target-disease” network model. UPLC-Q-TOF/MS technology in combination with multivariate statisticalmethods had certain advantages in studying the complex changes of chemical composition before and after manufacturing pulveratumof SE. It provided a basis for clarifying the toxicity-attenuated mechanisms of SE manufacturing pulveratum, and laid the foundation for its further development and utilization.

Key words: Semen Euphorbiae, Semen Euphorbiae Pulveratum, UPLC-Q-TOF/MS, Multivariate statistical techniques, Chemical constituents, Manufacturing pulveratum

中图分类号:

R284

Supporting:

王慧楠, 张景珍, 崔曰新, 王思雨, 高慧, 张瑶, 王新杰, 杨子烨, 陈梦雨, 王佩华, 张桂梅, 王英姿, 张超. 千金子制霜前后UPLC-Q-TOF/MS化学成分分析研究[J]. 中国药学(英文版), 2020, 29(7): 470-479.

Huinan Wang, Jingzhen Zhang, Yuexin Cui, Siyu Wang, Hui Gao, Yao Zhang, Xinjie Wang, Ziye Yang, Mengyu Chen, Peihua Wang, Guimei Zhang, Yingzi Wang, Chao Zhang. Chemical comparison of Semen Euphorbiae and Semen Euphorbiae Pulveratum by UPLC-Q-TOF/MS coupled with multivariate statistical techniques[J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(7): 470-479.

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千金子制霜前后UPLC-Q-TOF/MS化学成分分析研究

Chemical comparison of Semen Euphorbiae and Semen Euphorbiae Pulveratum by UPLC-Q-TOF/MS coupled with multivariate statistical techniques

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