西多福韦体外抑制55型人腺病毒的感染

doi:10.5246/jcps.2023.06.040

中国药学(英文版) ›› 2023, Vol. 32 ›› Issue (6): 473-477.DOI: 10.5246/jcps.2023.06.040

西多福韦体外抑制55型人腺病毒的感染

王文博¹, 王梦珂²^,³, 胡宗海², 熊杰², 刘媛²^,^*()

1. 西部战区总医院疾病预防控制科, 四川成都 610083
2. 西部战区总医院检验科, 四川成都 610083
3. 成都中医药大学医学技术学院, 四川成都 610075

收稿日期:2022-12-23 修回日期:2023-01-06 接受日期:2023-01-23 出版日期:2023-07-01 发布日期:2023-07-01
通讯作者: 刘媛
作者简介:
+ Tel.: +86-28-86570385, E-mail: liuyuan198231@163.com
基金资助:
A Project from the Hospital (Grant No. 2021-XZYG-B11) and the Key Research and Development Project of the Sichuan Science and Technology Department (Grant No. 2019YFS0367).

Cidofovir inhibits human adenovirus type 55 infection in vitro

Wenbo Wang¹, Mengke Wang²^,³, Zonghai Hu², Jie Xiong², Yuan Liu²^,^*()

1 Department of Disease Control and Prevention, General Hospital of Western Theater Command, Chengdu 610083, Sichuan, China
2 Department of Clinical Laboratory, General Hospital of Western Theater Command, Chengdu 610083, Sichuan, China
3 College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan, China

Received:2022-12-23 Revised:2023-01-06 Accepted:2023-01-23 Online:2023-07-01 Published:2023-07-01
Contact: Yuan Liu

摘要/Abstract

摘要：

55型人腺病毒 (HAdV-55) 通常引起急性呼吸道感染疾病, 目前临床上还没有有效的抗病毒药物。本研究通过体外实验筛选了14种核苷酸类似物的抗HAdV-55活性。结果发现, 西多福韦在HEp-2细胞上能够抑制HAdV-55的感染, 为其用于HAdV-55感染疾病的治疗研究提供了依据。

关键词: 西多福韦, 阿德福韦, 替诺福韦, 腺病毒, HAdV-55

Abstract:

Human adenovirus type 55 (HAdV-55) can usually cause acute respiratory diseases. Unfortunately, there is no available specific antiviral drug. In the present study, 14 nucleotide analogs were screened for their anti-HAdV-55 activities in vitro. The results suggested that cidofovir could inhibit HAdV-55 infection in HEp-2 cells, providing strong evidence for further research on treating HAdV-55 infection.

Key words: Cidofovir, Adefovir, Tenofovir, Adenovirus, HAdV-55

Supporting: /attached/file/20230702/20230702111129_565.pdf

王文博, 王梦珂, 胡宗海, 熊杰, 刘媛. 西多福韦体外抑制55型人腺病毒的感染[J]. 中国药学(英文版), 2023, 32(6): 473-477.

Wenbo Wang, Mengke Wang, Zonghai Hu, Jie Xiong, Yuan Liu. Cidofovir inhibits human adenovirus type 55 infection in vitro[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(6): 473-477.

图/表 1

Figure 1. Anti-HAdV-55 activities of NAs. (A) HEp-2 cells were infected with 107 copies/mL of the HAdV-55 virus in the presence of 100 μmol/L NAs. RT-qPCR was adopted to determine intracellular HAdV RNA levels at 48 h post-infection. (B & C) HEp-2 cells were infected with a lethal dose of the HAdV-55 virus in the presence of 100 μmol/L NAs. CPE inhibition was observed by crystal violet stain at 48 h post-infection (B). Cell viability was determined by a CCK8 kit (C). (D) HEp-2 cells were infected with 107 copies/mL of HAdV-3, 7, 14, and 55 viruses in the presence of 100 μmol/L NAs. RT-qPCR was adopted to determine intracellular HAdV RNA levels at 48 h post-infection. Data were shown as percentages compared with VC groups. VC and CC represent virus control and cell control, respectively. Data in (A), (C) & (D) were expressed as mean ± SD (*P < 0.05, compared with DMSO or VC).

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西多福韦体外抑制55型人腺病毒的感染

Cidofovir inhibits human adenovirus type 55 infection in vitro

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