菝葜总黄酮主成分鉴定及苯酚胶浆诱导大鼠盆腔炎的抗炎作用评价

doi:10.5246/jcps.2021.02.013

中国药学(英文版) ›› 2021, Vol. 30 ›› Issue (2): 157-168.DOI: 10.5246/jcps.2021.02.013

菝葜总黄酮主成分鉴定及苯酚胶浆诱导大鼠盆腔炎的抗炎作用评价

陈佳倩, 金晨, 徐博伦, 詹煌, 付容容, 李风琴, 黄慧莲^*()

江西中医药大学现代中药制剂教育部重点实验室, 江西南昌 330004

收稿日期:2020-09-08 修回日期:2020-11-15 接受日期:2020-12-09 出版日期:2021-02-28 发布日期:2021-02-27
通讯作者: 黄慧莲
作者简介:
+ Tel.: +86-791-87118658, E-mail: huilianh@163.com
基金资助:
The Science and Technology Department of Jiangxi Province (Grant No. 20192ACB20016, 20165BCB18006, 20172BCB22010, GJJ201214) and the National Natural Science Foundation of China (NSFC, Grant No. 8150640).

Identification of major compounds of total flavonoids from Smilax china and evaluation of anti-inflammatory effect on phenol mucilage-induced pelvic inflammation in rats

Jiaqian Chen, Chen Jin, Bolun Xu, Huang Zhan, Rongrong Fu, Fengqin Li, Huilian Huang^*()

Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China

Received:2020-09-08 Revised:2020-11-15 Accepted:2020-12-09 Online:2021-02-28 Published:2021-02-27
Contact: Huilian Huang

摘要/Abstract

摘要：

菝葜是治疗盆腔炎(PID)的传统中药, 其中的黄酮类化合物已被确定为抗炎的主要活性成分。然而, 目前尚未从菝葜中提取出高含量的总黄酮, 总黄酮的作用机制也不明确。本研究目的是提取得到菝葜中的总黄酮, 并阐明其对大鼠PID的作用机制。本文首次在菝葜中获得高含量(达到55.6%)的总黄酮, 分离纯化后通过NMR鉴定了其中的15个化合物。菝葜总黄酮能降低慢性盆腔炎大鼠子宫组织中IL-6和IL-1β的水平, 这可能是菝葜活性成份治疗慢性盆腔炎症和缓解盆腔粘连的药理作用机制之一。

关键词: 菝葜, 总黄酮, 核磁共振, 盆腔炎

Abstract:

As a Chinese medicinal plant, Smilax china is traditionally used in the treatment of pelvic inflammatory disease (PID). Flavonoids have been identified as the anti-inflammatory bioactive fraction. However, the high content of total flavonoids has not been extracted from S. china. The therapeutic effects and mechanisms of total flavonoids are still unknown. In the present study, we aimed to obtain the effective parts of flavonoids in S. china, and elucidate the mechanism of action on PID. The anti-inflammatory effect was evaluated on phenol mucilage-induced pelvic inflammation in rats. This was the first time that total flavonoids in high concentration (up to 55.6%) were obtained in S. china. A total of 15 compounds were separated and identified by NMR, and the total flavonoids were detected with HPLC. TFSC caused a reduction in serum levels of IL-6 and IL-1β. These results suggested that TFSC had a significant anti-PID effect, probably due to inhibition of the inflammatory reaction.

Key words: Smilax china, Total flavonoids, NMR, Pelvic inflammation disease

Supporting:

陈佳倩, 金晨, 徐博伦, 詹煌, 付容容, 李风琴, 黄慧莲. 菝葜总黄酮主成分鉴定及苯酚胶浆诱导大鼠盆腔炎的抗炎作用评价[J]. 中国药学(英文版), 2021, 30(2): 157-168.

Jiaqian Chen, Chen Jin, Bolun Xu, Huang Zhan, Rongrong Fu, Fengqin Li, Huilian Huang. Identification of major compounds of total flavonoids from Smilax china and evaluation of anti-inflammatory effect on phenol mucilage-induced pelvic inflammation in rats[J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(2): 157-168.

图/表 5

Figure 1. The structures of compounds in total flavonoids of Smilax china.

Figure 2. High Performance Liquid Chromatography (HPLC) of total flavonoids fraction from Smilax china L. rhizome (TFSC). 1. Neoastilbin; 2. (+)-dihydrokaempferol; 3. engeletin; 4. astilbin; 5. neoisoastilbin; 6. 3'-methoxyl-quercetin-3-O-α-L-rhamnopyranoside; 7. quercetin3-O-α-L-rhamnopyranoside; 8. catechin; 9. (2R,3R)-taxifolin.

Figure 3. Morphology of the uteri. (a) The background control group; (b) The sham-operated group; (c) The model group; (d) Fuke Qianjin Tablet group; (e) Jingangteng Syrup group; (f) TFSC low-dose group; (g) TFSC middle-dose group; (h) TFSC high-dose group.

Figure 4. HE staining of the left uterus. (a) The background control group: there was no inflammatory cell infiltration, intimal hyperemia and edema; (b) The sham-operated group: there was no inflammatory cell infiltration, intimal hyperemia and edema; (c) The model group: epithelial cells degeneration and necrosis; (d) Fuke Qianjin Tablet group: cavity wall structure is normal, no inflammatory cell infiltration; (e) Jingangteng Syrup group: chronic inflammatory cells are scattered in focal areas and go deep into the musculature, with severe hyperemia and edema in the intima; (f) TFSC low-dose group: cavity wall structure is normal; (g) TFSC middle-dose group: the mucous lamina propria of lumen wall structure decreased, epithelial cell shedding; (h) TFSC high-dose group: the mucous lamina propria of lumen wall structure decreased.

Table 1. The effects of TFSC, Fuke Qianjin Tablet and Jingangteng syrup on serum IL-6 and IL-1β in rats ( x ± s, n = 6).

参考文献 26

[1]	Mitchell, C.; Prabhu, M. Pelvic inflammatory disease: current concepts in pathogenesis, diagnosis and treatment. Infect. Dis. Clin. North Am. 2013, 27, 793–809.
[2]	Fan, S.R.; Li, T. CDC 2015 guideline for the diagnosis and treatment of pelvic inflammatory disease. Chin. Gen Pract. 2015, 30, 3641–3643.
[3]	Duarte, R.; Fuhrich, D.; Ross, J.D.C. A review of antibiotic therapy for pelvic inflammatory disease. Int. J. Antimicrob. Agents. 2015, 46, 272–277.
[4]	Jiang, J.Y.; Xu, Q. Immunomodulatory activity of the aqueous extract from rhizome of Smilax glabra in the later phase of adjuvant-induced arthritis in rats. J. Ethnopharmacol. 2003, 85, 53–59.
[5]	Navarro, M.C.; Montilla, M.P.; Cabo, M.M.; Galisteo, M.; Cáceres, A.; Morales, C.; Berger, I. Antibacterial, antiprotozoal and antioxidant activity of five plants used in Izabal for infectious diseases. Phytother. Res. 2003, 17, 325–329.
[6]	Shu, X.S.; Gao, Z.H.; Yang, X.L. Anti-inflammatory and anti-nociceptive activities of Smilax China L. aqueous extract. J. Ethnopharmacol. 2006, 103, 327–332.
[7]	Lü, Y.; Chen, D.S.; Deng, J.G.; Tian, L.Q. Effect of Smilax China on adjunctive arthritis mouse. J. Chin. Med. Mater. 2003, 26, 344–346.
[8]	Lee, S.E.; Ju, E.M.; Kim, J.H. Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax China root. Exp. Mol. Med. 2001, 33, 263–268.
[9]	CNP Committee of National Pharmacopoeia, "China Pharmacopoeia", Chemical Industry Press, Beijing, 2015, 290.
[10]	Wu, L.S.; Wang, X.J.; Wang, H.; Yang, H.W.; Jia, A.Q.; Ding, Q. Cytotoxic polyphenols against breast tumor cell in Smilax China L. J. Ethnopharmacol. 2010, 130, 460–464.
[11]	Khan, I.; Nisar, M.; Ebad, F.; Nadeem, S.; Saeed, M.; Khan, H.; Samiullah, Khuda, F.; Karim, N.; Ahmad, Z. Anti-inflammatory activities of Sieboldogenin from Smilax China Linn.: experimental and computational studies. J. Ethnopharmacol. 2009, 121, 175–177.
[12]	Luo, Y.Q.; Ma, Y.; Song, L.Y.; Luo, H.C.; Hou, L.B. Effect of Smilax China bioactive fraction on tumor necrosis factor-α and interleukin-4 contents in uterine tissue of rats with chronic pelvic inflammatory disease. J. Southern Med. Univ. 2014, 34, 236–240.
[13]	Ding, L.S.; Liang, Q.L.; Teng, Y.F. Study on flavonoids in seeds of Hovenia dulcis. Acta. Pharm. Sin. 1997, 32, 600–602.
[14]	Xu, J.; Li, X.; Zhang, P.; Li, Z.L.; Wang, Y. Antiinflammatory constituents from the roots of smilax bockii warb. Arch. Pharmacal. Res. 2005, 28, 395–399.
[15]	Zhang, H.S.; Kang, L.P.; Zou, P.; Han, L.F.; Xiong, C.Q.; Zhao, Yang.; Tan, D.W.; Ma, BP.; Wen, D.J. Chemical constituents from Smilax davidiana. Chin. Tradit. Herbal. Drugs. 2009, 40, 11–14.
[16]	Shen, S.M.; Zhang, J.; Li, G.Z.; Liu, C.M.; Lu, H.; Si, J.Y. Chemical constituents of the aerial parts of Emilia sonchifolia (II). Chin. Pharmacol. J. 2013, 48, 1815–1819.
[17]	Konishi, T.; Yamazoe, K.; Kanzato, M.; Konoshima, T.; Fujiwara, Y. Three diterpenoids (excoecarins V1: V3) and a flavanone glycoside from the fresh stem of excoecaria agallocha. Chem. Pharm. Bull. 2003, 51, 1142–1146.
[18]	Chai, X.Y.; Li, P.; Tang, L.Y. Studies on chemical constituents in dried buds of Lonicera confusa. China J. Chin. Mater. Med. 2004, 29, 865–867.
[19]	Kasai, R.; Hirono, S.; Chou, W.H.; Tanaka, O.; Chen, F.H. Sweet dihydroflavonol rhamnoside from leaves of Engelhardtia chrysolepis, a Chinese folk medicine, hung-qi. Chem. Pharm. Bull. 1988, 36, 4167–4170.
[20]	Zhou, Z.H.; Zhang, Y.J.; Yang, C.R. Saluenin, a new flavonol glycoside from Camellia saluenensis. Acta. Bot. Yunnan. 2000, 22, 90–96.
[21]	Yang, G.X.; Hu, C.Q. A novel stilbenolignan from Caragana rosea Turcz. Chin. J. Org. Chem. 2003, 8, 873.
[22]	Lu, Y.R.; Foo, L.Y. The polyphenol constituents of grape pomace. Food Chem. 1999, 65, 1–8.
[23]	Kulesh, N.I.; Denisenko, V.A.; Maksimov, O.B. Stilbenolignan from Maackia amurensis. Phytochemistry. 1995, 40, 1001–1003.
[24]	Chen, G.L.; Li, X.L.; Shi, L.G.; Zhang, S.J. Chemical constituents from Cirsium pendulum Fisch. ex DC. J. Chin. Med. Mater. 2007, 30, 291–294.
[25]	Zhang, W.J.; Liu, Y.Q.; Li, X.C. Chemical constituents of ecological tea from chemical constituents of "EcologicalTea" from Yunnan. Acta. Bot. Yunnan. 1995, 17, 204–208.
[26]	Nonaka, G.; Nishioka, I. Tannins and related compounds. VII. phenylpropanoid-substituted epicatechins, cinchonains from cinchona succirubra. (1). Chem. Pharm. Bull. 1982, 30, 4268–4276.

菝葜总黄酮主成分鉴定及苯酚胶浆诱导大鼠盆腔炎的抗炎作用评价

Identification of major compounds of total flavonoids from Smilax china and evaluation of anti-inflammatory effect on phenol mucilage-induced pelvic inflammation in rats

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 26

相关文章 13

编辑推荐

Metrics

本文评价

[1]	李雪娇, 董建伟, 高秀, 李贵军, 史俊友, 张艳清. 定量核磁共振氢谱法对徐长卿中丹皮酚含量的快速提取和含量测定[J]. 中国药学(英文版), 2020, 29(6): 422-430.
[2]	刘晓婷, 徐静娜, 肖坤, 郭强胜, 许旭. 固相萃取-定量核磁共振波谱法测定双黄连胶囊中的绿原酸[J]. 中国药学(英文版), 2020, 29(4): 227-235.
[3]	王刊, 付宏征^*. 肥皂荚Gymnocladus chinensis Baill.中两个三萜皂苷的结构鉴定[J]. , 2009, 18(2): 141-145.
[4]	胡道德*, 姚慧娟, 顾磊, 王松坡, 刘皋林. 淫羊藿总黄酮对大鼠肝微粒体CYP1A2、CYP3A4和CYP2E1活性的影响[J]. , 2008, 17(2): 167-169.
[5]	刘火安, 王伯初, 戴传云^*, 邵志勇, 何从林, 贾云. 利用大孔树脂从葛根中分离纯化总黄酮[J]. , 2006, 15(2): 121-126.
[6]	杨春晖, 李勤, 刘雪辉, 赵兴凯, 崔育新^*. 氨氯地平和利培酮的¹H和¹³C核磁共振信号归属[J]. , 2004, 13(1): 49-52.
[7]	张建业, 杨秀伟^*. 罗汉果皂苷IVa碳氢NMR信号的归属[J]. , 2003, 12(4): 196-200.
[8]	孙静, 李勤, 乔梁, 崔育新^* . 莫沙必利的核磁共振氢谱与碳谱指定[J]. , 2003, 12(2): 98-100.
[9]	翟光喜^*, 娄红祥, 毕殿洲, 邹立家. 固体分散体中葛根素与磷脂的相互作用[J]. , 2003, 12(1): 36-40.
[10]	胡春, 童嘉, 谭日红, 李虹, 赵水梅, 王圣符. 二叔丁基二氯化锡与含氮配体的配合物的合成、表征和体外抗癌活性研究[J]. , 1999, 8(2): 111-114.
[11]	崔育新, 张虎翼, 刘雪辉, 马灵台, 张礼和^*. 氟代异碳核苷衍生物的NMR研究[J]. , 1999, 8(1): 39-42.
[12]	崔育新, 刘晓峰, 吴海涛, 潘鑫复. 苯并硫氮杂卓及其环加成产物的2D NMR 研究[J]. , 1998, 7(4): 178-181.
[13]	陈亚民, 卢英华, 陈雁君, 马存升, 于德泉. 用二维核磁共振法研究肿足蕨碱的结构[J]. , 1992, 1(2): 1-6.