柴胡的HPLC-ELSD指纹图谱研究

doi:10.5246/jcps.2013.01.005

柴胡的HPLC-ELSD指纹图谱研究

周勇, 王延亮, 马志桥, 王邠, 梁鸿, 赵玉英, 张庆英^*

北京大学医学部天然药物及仿生药物国家重点实验室; 药学院天然药物学系, 北京 100191

收稿日期:2012-09-23 修回日期:2012-11-10 出版日期:2013-01-20 发布日期:2013-01-20
通讯作者: 张庆英*

Chromatographic fingerprint analysis of Bupleuri Radix by HPLC-ELSD

Yong Zhou, Yanliang Wang, Zhiqiao Ma, Bin Wang, Hong Liang, Yuying Zhao, Qingying Zhang^*

State Key Laboratory of Natural and Biomimetic Drugs; Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2012-09-23 Revised:2012-11-10 Online:2013-01-20 Published:2013-01-20
Contact: Qingying Zhang*

摘要/Abstract

摘要： 本文建立了一种简单、有效的柴胡药材的高效液相色谱-蒸发光散射 (HPLC-ELSD) 指纹图谱方法, 并对不同产地和不同品种的柴胡药材及其饮片进行了系统评价。研究结果显示南柴胡药材和市售柴胡饮片的化学成分变化较大, 而北柴胡药材的化学成分则相对稳定, 且南柴胡药材与北柴胡药材化学成分差别明显, 提示南柴胡药材作为柴胡应用要谨慎。另外与柴胡药材相比, 柴胡饮片的指纹图谱也发生了变化, 尤其是保留时间在30-40分钟之间和45分钟之后色谱峰变化尤为明显, 提示在炮制过程中可能发生了化学成分的变化。最终本文确定了柴胡中12个主要皂苷类成分的色谱指纹峰, 并建议柴胡皂苷a、c和d 为其基本特征峰, 这对于柴胡的品种鉴定和质量控制具有指导意义。

关键词: 柴胡, 北柴胡, 南柴胡, 化学指纹图谱, HPLC-ELSD, 柴胡皂苷

Abstract:

To facilitate the species identification and quality assessment of Chaihu (Bupleuri Radix), a simple and valid chromatographic fingerprint method was developed. The method uses high-performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD) and the data analysis is assisted by professional analytical software recommended by the State Food and Drug Administration (SFDA). The results indicate that Nan Chaihu raw materials and Chaihu decoction pieces vary markedly in chemical quality, while Bei Chaihu raw materials are relatively more stable. Furthermore, it is obvious that Nan Chaihu is chemically very different from Bei Chaihu, suggesting that Nan Chaihu may not be suitable for medicinal use. In addition, the obvious differences between the chromatograms of decoction pieces and raw materials, especially the peaks between 30 and 40 min and after 45 min, indicate possible effects of the processing procedures on the chemicals. By analyzing the fingerprints of all samples, 12 main saponin-like fingerprint peaks, of which at least three are characteristic peaks of saikosaponins a, c, and d, are proposed to be considered for further characterization and quality evaluation of Chaihu.

Key words: Bupleuri Radix, Bupleurum chinense, Bupleurum scorzonerifolium, Chromatographic fingerprint, HPLC-ELSD, Saikosaponins

中图分类号:

R284.1

Supporting:

Foundation items: The project of Quality Standards for Chinese Medicines and Information System Platform (Grant No. 2009ZX09308-004) and National Natural Science Foundation of China (Grant No. 21172008).
^*Corresponding author. Tel.: 86-10-82801725

周勇, 王延亮, 马志桥, 王邠, 梁鸿, 赵玉英, 张庆英^*. 柴胡的HPLC-ELSD指纹图谱研究[J]. , DOI: 10.5246/jcps.2013.01.005.

Yong Zhou, Yanliang Wang, Zhiqiao Ma, Bin Wang, Hong Liang, Yuying Zhao, Qingying Zhang^*. Chromatographic fingerprint analysis of Bupleuri Radix by HPLC-ELSD[J]. , DOI: 10.5246/jcps.2013.01.005.

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