HPLC-DAD-ELSD法同时测定小柴胡汤正丁醇有效部位中7种成分的含量

doi:10.5246/jcps.2015.04.031

中国药学(英文版) ›› 2015, Vol. 24 ›› Issue (4): 236-240.DOI: 10.5246/jcps.2015.04.031

HPLC-DAD-ELSD法同时测定小柴胡汤正丁醇有效部位中7种成分的含量

原红霞^1,2, 李慧峰¹, 裴妙荣^1*

1. 山西中医学院, 山西太原 030024
2. 山西医科大学, 山西太原 030001

收稿日期:2015-01-14 修回日期:2015-02-02 出版日期:2015-04-28 发布日期:2015-02-22
通讯作者: Tel.: 86-351-2272281
基金资助:
Foundation for Young Scholars of Shanxi Province, China (Grant No. 2011021007-2).

Simultaneous determination of seven active components in n-butanol effective fraction of Xiao Chai Hu Tang by HPLC-DAD-ELSD

Hongxia Yuan^1,2, Huifeng Li¹, Miaorong Pei^1*

1. Shanxi University of Traditional Chinese Medicine, Taiyuan 030024,China
2. School of Public Health Science, Shanxi Medical University, Taiyuan 030001, China

Received:2015-01-14 Revised:2015-02-02 Online:2015-04-28 Published:2015-02-22
Contact: Tel.: 86-351-2272281
Supported by:
Foundation for Young Scholars of Shanxi Province, China (Grant No. 2011021007-2).

摘要/Abstract

摘要：

本文建立了同时测定小柴胡汤正丁醇有效部位中甘草苷、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素、人参皂苷Re和人参皂苷Rb₁含量的HPLC-DAD-ELSD法。采用Diamonsil C₁₈₍₂₎色谱柱(4.6 mm×250 mm, 5.0 μm), 以乙腈–0.05%甲酸水溶液为流动相, 梯度洗脱, 流速1 mL/min, 二极管阵列检测器的检测波长为276 nm, 蒸发光散射检测器的漂移管温度为110ºC, 载气流速氮气2.1 L/min, 柱温为30 ºC。结果表明: 7种有效成分在一定范围内线性关系良好。该方法简便、快速、重复性好, 可用于同时测定小柴胡汤正丁醇有效部位中7种有效成分的含量。

关键词: HPLC-DAD-ELSD, 正丁醇有效部位, 小柴胡汤, 同时测定

Abstract:

An HPLC-DAD-ELSD method was developed and validated for the simultaneous quantitation of seven active components (liquiritin, baicalin, wogonoside, baicalein, wogonin, ginsenosides Re and ginsenosides Rb₁) in n-butanol effective fraction of Xiao Chai Hu Tang. A Diamonsil C₁₈₍₂₎ column (4.6 mm × 250 mm, 5 μm) was used as the stationary phase and the mobile phase was consisted of acetonitrile and aqueous phosphate acid (0.05%, v/v). Gradient elution was carried out at the flow rate of 1 mL/min. The detection wavelength was set at 276 nm and an evaporative light scattering detector was also used. Good linearity for all the seven active components was observed. The established method is simple, fast, reliable, and suitable for the quality control of n-butanol effective fraction of Xiao Chai Hu Tang.

Key words: HPLC-DAD-ELSD, n-Butanol effective fraction, Xiao Chai Hu Tang, Simultaneous determination

中图分类号:

R917

Supporting:

原红霞, 李慧峰, 裴妙荣. HPLC-DAD-ELSD法同时测定小柴胡汤正丁醇有效部位中7种成分的含量[J]. 中国药学(英文版), 2015, 24(4): 236-240.

Hongxia Yuan, Huifeng Li, Miaorong Pei. Simultaneous determination of seven active components in n-butanol effective fraction of Xiao Chai Hu Tang by HPLC-DAD-ELSD[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(4): 236-240.

[1]	焦豪妍, 徐胜梅, 范春林, 张庆文, 王英. 高良姜指纹图谱的建立及主要成分的定量分析[J]. 中国药学(英文版), 2019, 28(10): 728-738.
[2]	范辉, 郭姣^*, 陈媛媛. HPLC-DAD-ELSD法同时测定复方贞术调脂胶囊15个活性成分[J]. , 2013, 22(1): 47-54.

HPLC-DAD-ELSD法同时测定小柴胡汤正丁醇有效部位中7种成分的含量

Simultaneous determination of seven active components in n-butanol effective fraction of Xiao Chai Hu Tang by HPLC-DAD-ELSD

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价