高良姜指纹图谱的建立及主要成分的定量分析

doi:10.5246/jcps.2019.10.069

中国药学(英文版) ›› 2019, Vol. 28 ›› Issue (10): 728-738.DOI: 10.5246/jcps.2019.10.069

高良姜指纹图谱的建立及主要成分的定量分析

焦豪妍¹, 徐胜梅^2,3, 范春林^2,3, 张庆文⁴, 王英^2,3*

1. 广东食品药品职业学院药学院, 广东广州 510520
2. 暨南大学药学院中药及天然药物研究所, 广东广州 510632
3. 暨南大学暨南大学-香港科技大学神经科学和创新药物研究联合实验室, 广东广州 510632
4. 澳门大学中华医药研究院, 澳门 999078

收稿日期:2019-08-27 修回日期:2019-09-19 出版日期:2019-10-31 发布日期:2019-09-30
通讯作者: Tel.: +86-20-85223553, E-mail: wangying_cpu@163.com
基金资助:
The National Natural Science Foundation of China (Grant No.81503210), the Medical Scientific Research Foundation of Guangdong Province (Grant No.B2017049), the Natural Science Foundation of Guangdong Food and Drug Vocational College (Grant No. 2016YZ003).

Chromatographic fingerprint analysis and quantitative evaluate the rhizomes of Alpinia officinarum Hance (lesser galangal)

Haoyan Jiao¹, Shengmei Xu^2,3, Chunlin Fan^2,3, Qingwen Zhang⁴, Ying Wang^2,3*

1. School of Pharmacy, Guangdong Food and Drug Vocational College, Guangzhou 510520, China
2. Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510000, China
3. JNU-HKUST Joint Laboratory for Neuroscience & Innovative Drug Research, Jinan University, Guangzhou 510000, China
4. Institute of Chinese Medical Sciences, University of Macau, Macao 999078, China

Received:2019-08-27 Revised:2019-09-19 Online:2019-10-31 Published:2019-09-30
Contact: Tel.: +86-20-85223553, E-mail: wangying_cpu@163.com
Supported by:
The National Natural Science Foundation of China (Grant No.81503210), the Medical Scientific Research Foundation of Guangdong Province (Grant No.B2017049), the Natural Science Foundation of Guangdong Food and Drug Vocational College (Grant No. 2016YZ003).

摘要/Abstract

摘要：

高良姜是著名的中药材, 临床上广泛用于治疗胃肠道疾病。本研究建立了一种简便、快速的HPLC-DAD方法对高良姜进行质量控制。建立基于15个不同产地高良姜样品的指纹图谱, 采用相似性分析(SA)、层次聚类分析(HCA)和主成分分析法(PCA)法对不同产地的高良姜药材及指标成分进行分析, 采用HPLC-ESI-MS法对主要成分进行鉴定。最终确定了12个共有峰, 并对其中的10个主要色谱峰进行了明确的化学指认, 建立了6个活性组分(5R)-5-羟基-1-苯基-7-(4-羟基-3-甲氧基苯基)-3-庚酮(DPHA)、乔松素、高良姜素、高良姜素-3-甲醚、1-苯基-7-(4-羟基-3-甲氧基苯基)-4-烯-3-庚酮(DPHB)和(5R)-5-羟基-1,7-二苯-3-庚酮(DPHC)的同时含量测定方法。该方法具有良好的分离度、精密度和回收率。结果表明, 色谱指纹图谱结合多组分含量测定法是一种适合于高良姜药材质量评价的分析方法。

关键词: 高良姜, 同时测定, 色谱指纹图谱, 质量评价, 中药

Abstract:

The rhizome of Alpinia officinarum Hance is a well-known traditional Chinese medicine (TCM) and has been widely used for the remedy of gastrointestinal diseases. In the present study, a simple and rapid HPLC-DAD was developed for the qualitycontrol of the rhizomes of A. officinarum. Its chemical fingerprint was established using raw material of 15 different origins in China. Similarity analysis (SA) and hierarchical clusting analysis (HCA) were applied to select the qualitative markers. Principal components analysis (PCA) was conducted to select the quantitative markers of the rhizomes of A. officinarum samples from different origins. The constituents were confirmed by (+) electrospray ionization LC-MS. 12constituents were selected as common peaks and 10 of them were confirmed by (+) electrospray ionization LC-MS. Six bioactive constituents including DPHA, galangin flavanone, galangin, galangin 3-methylether, DPHB and DPHC were simultaneous determination by using the HPLC-DAD analysis. The developed method was able to determine the bioactive components with excellent resolution, precision and recovery. The results indicated that chromatographic fingerprint combination with multi-components determination method is suitable for quality assessment of the rhizomes of A. officinarum.

Key words: Alpinia officinarum Hance, Simultaneous determination, Chromatographic fingerprint, Quality evaluation, Traditional Chinese medicine

中图分类号:

R284

Supporting:

Supplementary Table 1. The regression data, LODs and LOQs for six bioactive compounds analyzed by HPLC.

^a LOD refers to the limits of detection, S/N = 3; ^b LOQ refers to the limits of quantity, S/N = 10.

Supplementary Table 2. Intra-day and inter-day precision of six bioactive compounds.

^a Intra-day precision on 1 day for tested five times; ^b Inter-day precision on 3 different days.

Supplementary Table 3. Reproducibility and stability of six bioactive compounds.

^a Content = mean±S.D.; ^b Percentage decrease (%): [the content (mg g^–1)of prepared sample of Alpinia officinarum (batch no. 01) at 0 h – those at 24 h/those at 0 h] × 100.

Supplementary Table 4. Recovery of the six bioactive compounds.

^a Recovery (%) = [(found amount – original amount)/spiked amount]×100.

Supplementary Table 5. The relative retention time (RRT±SD) and the relative peak area (RPA±SD) of 12 characteristic peaks in chromatograms of 15 samples.

^a RRT: the ratio between peak retention time of target and internal reference substance; ^b RPA: the ratio between peak area of target and internal reference substance; ^cThe internal reference substance.

焦豪妍, 徐胜梅, 范春林, 张庆文, 王英. 高良姜指纹图谱的建立及主要成分的定量分析[J]. 中国药学(英文版), 2019, 28(10): 728-738.

Haoyan Jiao, Shengmei Xu, Chunlin Fan, Qingwen Zhang, Ying Wang. Chromatographic fingerprint analysis and quantitative evaluate the rhizomes of Alpinia officinarum Hance (lesser galangal)[J]. Journal of Chinese Pharmaceutical Sciences, 2019, 28(10): 728-738.

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高良姜指纹图谱的建立及主要成分的定量分析

Chromatographic fingerprint analysis and quantitative evaluate the rhizomes of Alpinia officinarum Hance (lesser galangal)

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