中药九里香两种不同基原植物的药效学比较研究

doi:10.5246/jcps.2021.01.005

中国药学(英文版) ›› 2021, Vol. 30 ›› Issue (1): 49-57.DOI: 10.5246/jcps.2021.01.005

中药九里香两种不同基原植物的药效学比较研究

鹿梦秋, 梁海珍, 屠鹏飞, 姜勇^*()

北京大学医学部药学院天然药物及仿生药物国家重点实验室, 北京 100191

收稿日期:2020-07-15 修回日期:2020-08-20 接受日期:2020-09-13 出版日期:2021-01-29 发布日期:2021-01-29
通讯作者: 姜勇
作者简介:
+ Tel.: +86-10-82802719, E-mail: yongjiang@bjmu.edu.cn
基金资助:
National Natural Science Foundation of China (Grant Nos. 81773864, 81473106); National Key R&D Program of China (Grant No. 2019YFC1711000).

Pharmacodynamic comparison of two different source plants of Murrayae Folium et Cacumen

Mengqiu Lu, Haizhen Liang, Pengfei Tu, Yong Jiang^*()

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2020-07-15 Revised:2020-08-20 Accepted:2020-09-13 Online:2021-01-29 Published:2021-01-29
Contact: Yong Jiang

摘要/Abstract

摘要：

对中药九里香的两种基原植物九里香(Murraya exotica L.)和千里香(Murraya paniculata (L.) Jack)的药效进行对比研究, 为其多基原收载的合理性及临床用药的等同性提供参考。根据中药九里香的传统功效, 采用小鼠扭体反应模型、大鼠足肿胀模型、小鼠胃排空及小肠推进试验、大鼠急性血瘀模型,系统地评价两种植物的行气止痛、活血化瘀作用。结果显示,九里香和千里香的提取物均能明显抑制醋酸引起的小鼠扭体反应和角叉菜胶引起的大鼠足肿胀,减少足肿胀大鼠血浆中炎症因子IL-6, TNF-α和PGE2的水平,促进小鼠胃排空和小肠推进。上述药效呈剂量依赖性,并且相同剂量的九里香和千里香之间无显著差异。因此,九里香和千里香的提取物在抗炎镇痛和促进胃肠动力药效方面具有一致性和等效性,研究结果为中药九里香多基原植物的药效等效性提供了一定的依据。

关键词: 九里香, 千里香, 多基原中药, 药效, 一致性

Abstract:

A comparison of the pharmacodynamic effects of two source plants of Murrayae Folium et Cacumen (MFC), Murraya exotica L. and Murraya paniculata (L.) Jack, was performed in order to supply reference for its multi-source rationality and interchangeability in clinical practice. According to the traditional efficacy of MFC, the effects of promoting Qi, relieving pain, promoting blood circulation and removing blood stasis were systematically evaluated by the models of writhing response in mice, foot swelling in rats, gastric emptying and small intestine propulsion in mice, and acute blood stasis in rats, respectively. The results showed that both M. exotica and M. paniculata could significantly inhibit the writhing reaction induced by acetic acid in mice and the paw swelling induced by carrageenan in rats, reduce IL-6, TNF-α and PGE2 levels in plasma of paw-swelling rats and increase gastric empty rate and intestinal propulsive rate. The above-mentioned effects were dose-dependent, and there was no significant difference between M. exotica and M. paniculata at the same doses. Therefore, M. exotica and M. paniculata had the similar anti-inflammatory, analgesic and gastrointestinal motility promotion effects, which provided a support for the pharmacodynamic equivalence of the multi-source plants of MFC.

Key words: Murraya exotica L., Murraya paniculata (L.) Jack, Multi-source traditional Chinese medicine, Pharmacodynamics, Equivalence

Supporting:

鹿梦秋, 梁海珍, 屠鹏飞, 姜勇. 中药九里香两种不同基原植物的药效学比较研究[J]. 中国药学(英文版), 2021, 30(1): 49-57.

Mengqiu Lu, Haizhen Liang, Pengfei Tu, Yong Jiang. Pharmacodynamic comparison of two different source plants of Murrayae Folium et Cacumen[J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(1): 49-57.

图/表 6

Figure 1. HPLC fingerprints of M. exotica and M. paniculata. (A) M. exotica; (B) M. paniculata; (C) murrangatin. 1. minumicrolin, 2. murrangatin, 3. meranzin hydrate, 4. murpaniculol, 5. 5-hydroxy-7,3',4'-trimethoxyflavone, 6. auraptenol, 7. 5-hydroxy-7,3',4',5'-tetramethoxyflavone, 8. 5,7,8,3',4'-pentamethoxyflavone, 9. hainanmurpanin, 10. 5,7,3',4'-tetramethoxyflavone, 11. 5,7,8,3',4',5'-hexamethoxyflavone, 12. 5,6,7,3',4'-pentamethoxyflavone, 13. 5,7,3',4',5'-pentamethoxyflavone, 14. 5,6,7,3',4',5'-hexamethoxyflavone, 15. 5,6,7,8,3',4',5'-heptamethoxyflavone, 16. 5-hydroxy-6,7,3',4'-tetramethoxyflavone, 17. 5-hydroxy-6,7,3',4',5'-pentamethoxyflavone, 18. 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone, 19. 5-hydroxy-6,7,8,3',4',5'-hexamethoxyflavone.

Table 1. Effects of M. exotica and M. paniculata on writhing number of mice (n = 10, x ± SD).

Table 2. Effects of M. exotica and M. paniculata on paw swelling degree of rats (n = 10, x ± SD).

Table 3. Effects of M. exotica and M. paniculata on inflammatory cytokines in paw swelling rats (n = 10, x ± SD).

Table 4. Effects of M. exotica and M. paniculata on gastric emptying and small intestinal propulsion rate in mice (n = 10, x ± SD).

Table 5. Effects of M. exotica and M. paniculata on PT, APTT, TT and Fbg-T in rats (n = 8, x ± SD).

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中药九里香两种不同基原植物的药效学比较研究

Pharmacodynamic comparison of two different source plants of Murrayae Folium et Cacumen

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