乌拉尔甘草地上部分的化学成分及其PTP1B与α-葡萄糖苷酶抑制活性研究

doi:10.5246/jcps.2020.05.028

中国药学(英文版) ›› 2020, Vol. 29 ›› Issue (5): 305-313.DOI: 10.5246/jcps.2020.05.028

• 【研究论文】 • 下一篇

乌拉尔甘草地上部分的化学成分及其PTP1B与α-葡萄糖苷酶抑制活性研究

范靖然^1,2, 董泽源², 匡易², 周艳霞², 乔雪², 叶敏^2*

1. 北京中医药大学中药学院, 北京 100029
2. 北京大学药学院天然药物及仿生药物国家重点实验室, 北京 100191

收稿日期:2020-03-14 修回日期:2020-04-15 出版日期:2020-05-31 发布日期:2020-05-10
通讯作者: Tel.: +86-10-82801516, Fax: +86-10-82802024, E-mail: yemin@bjmu.edu.cn
基金资助:
National Natural Science Foundation of China (Grant No. 81891010/81891011), National Mega-project for Innovative Drugs (Grant No. 2018ZX09711001-008-003), and Science & Technology Department of Xinjiang Uygur Autonomous Region (Grant No. 2018AB012).

Chemical constituents of the aerial parts of Glycyrrhiza uralensis and their inhibitory activities against PTP1B and α-glucosidase

Jingran Fan^1,2, Zeyuan Dong², Yi Kuang², Yanxia Zhou², Xue Qiao², Min Ye^2*

1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
2. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2020-03-14 Revised:2020-04-15 Online:2020-05-31 Published:2020-05-10
Contact: Tel.: +86-10-82801516, Fax: +86-10-82802024, E-mail: yemin@bjmu.edu.cn
Supported by:
National Natural Science Foundation of China (Grant No. 81891010/81891011), National Mega-project for Innovative Drugs (Grant No. 2018ZX09711001-008-003), and Science & Technology Department of Xinjiang Uygur Autonomous Region (Grant No. 2018AB012).

摘要/Abstract

摘要：

从乌拉尔甘草地上部分分离得到11个化合物, 分别是glycyuralin Q (1), glycyuralin R (2), licoriphenone (3), 奥洛波尔(4), trifoliol (5), 7,2′,4′-三羟基-5-甲氧基-3-香豆素 (6), 11-羰基-9(Z),12(Z)-十八碳二烯酸 (7), 11-羰基-9(E),12(E)-十八碳二烯酸 (8),甘草利酮 (9), 格里西轮 (10), 2′-羟基刺芒柄花素 (11)。其中1和2为新化合物, 其结构采用1D、2D NMR及HR-MS等技术进行鉴定。分别筛选11个化合物对PTP1B及α-葡萄糖苷酶的抑制活性, 发现化合物1、2和10对PTP1B酶有较好的抑制活性, 其IC₅₀值分别是1.43、4.71和3.79 μM; 化合物2、4和10抑制α-葡萄糖苷酶的IC₅₀值分别是13.61、11.13和17.48 μM。

关键词: 地上部分, PTP1B酶, α-葡萄糖苷酶, 乌拉尔甘草

Abstract:

In the present study, a total of 11 compounds were isolated from the aerial parts of Glycyrrhiza uralensis, including two new compounds, glycyuralin Q (1) and glycyuralin R (2), and nine known compounds, including licoriphenone (3), orobol (4), trifoliol (5), 7,2′,4′-trihydroxy-5-methoxy-3-arylcoumarin (6), 11-hydroxy-9(Z),12(Z)-octadecadienoic acid (7), 11-hydroxy-9(E),12(E)-octadecadienoic acid (8), licoricone (9), glycyrin (10), and 2′-hydroxyformononetin (11). Structures of the new compounds were identified by 1D, 2D NMR and HR-MS data analyses. Compounds 1, 2 and 10 showed potent inhibitory activities against PTP1B, with IC₅₀ values of 1.43, 4.71 and 3.79 μM, respectively. Compounds 2, 4 and 10 inhibited α-glucosidase with IC₅₀ values of 13.61, 11.13 and 17.48 μM, respectively.

Key words: The aerial parts, PTP1B, α-Glucosidase, Glycyrrhiza uralensis

中图分类号:

R284

Supporting:

范靖然, 董泽源, 匡易, 周艳霞, 乔雪, 叶敏. 乌拉尔甘草地上部分的化学成分及其PTP1B与α-葡萄糖苷酶抑制活性研究[J]. 中国药学(英文版), 2020, 29(5): 305-313.

Jingran Fan, Zeyuan Dong, Yi Kuang, Yanxia Zhou, Xue Qiao, Min Ye. Chemical constituents of the aerial parts of Glycyrrhiza uralensis and their inhibitory activities against PTP1B and α-glucosidase[J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(5): 305-313.

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乌拉尔甘草地上部分的化学成分及其PTP1B与α-葡萄糖苷酶抑制活性研究

Chemical constituents of the aerial parts of Glycyrrhiza uralensis and their inhibitory activities against PTP1B and α-glucosidase

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