草木樨药材指纹图谱研究

草木樨药材指纹图谱研究

郑国华^#, 王桂红^#, 吴木琴, 黄志军, 陶君彦^*, 张晓昱, 张琼光, 肖飞, 李崇明

1. 湖北中医学院药学院, 湖北武汉 430065

2. 湖北中医学院附属医院门诊部, 湖北武汉 430061

3. 武汉健民集团, 湖北武汉 430054

4. 武汉大学生命科学院, 湖北武汉 430072

5. 华中科技大学生命科学与技术学院, 湖北武汉 430074

收稿日期:2008-08-15 修回日期:2009-02-10 出版日期:2009-03-15 发布日期:2009-03-15
通讯作者: 陶君彦*

Development of a fingerprint of Melilotus suaveolens Ledeb by high-performance liquid chromatography

Guo-Hua Zheng^#, Gui-Hong Wang^#, Mu-Qin Wu, Zhi-Jun Huang, Jun-Yan Tao^*, Xiao-Yu Zhang, Qiong-Guang Zhang, Fei Xiao, Chong-Ming Li

1. School of Pharmacy, Hubei College of Traditional Chinese Medicine, Wuhan 430065, China

2. Department of Affiliated Hospital, Hubei College of Traditional Chinese Medicine, Wuhan 430061, China

3. Hubei and Wuhan Engineering Technology center of Modernization Traditional Chinese Medicine, Wuhan Jianmin Pharmacy of TCM Co. Ltd, Wuhan 430054, China

4. State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China

5. College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China

Received:2008-08-15 Revised:2009-02-10 Online:2009-03-15 Published:2009-03-15
Contact: Jun-Yan Tao*

摘要/Abstract

摘要： 本文首次建立了一个简单而准确的高效液相色谱法指纹图谱方法, 对草木樨药材不同收获时间和不同的应用部分, 包括种子, 茎和整个地上部分进行了系统评价。结果采用线性梯度洗脱, 乙腈和0.1%磷酸水为流动相, 柱温25 °C, 检测波长为210 nm。该高效液相色谱指纹图谱表明, 不同药用部分有12个相同的共有峰和8个相同的共有指纹峰。说明高效液相色谱指纹图谱可用于草木樨属植物质量评价, 同时本文研究方法和思路也可用于其他中药的质量和分析研究。

关键词: 指纹图谱, 草木樨, 质量评价

Abstract: A simple and accurate high-performance liquid chromatography method (HPLC) was described to generate the metabolite fingerprints of Melilotus. suaveolens Ledeb of different harvesting times and different parts of the plant. A suitable chromatographic system was established using a linear gradient elution with acetonitrile and 0.1% phosphoric acid (H3PO4) as the mobile phase and a detection wavelength of 210 nm. HPLC analysis identified 12 common peaks and 8 common fingerprint peaks among the different parts of the herb. We found that the constituents varied at different harvesting times and in different parts of the plant. The HPLC fingerprint method could be used to evaluate the quality of M. suaveolens Ledeb and Melilotus genus herbs. The same strategy can also be applied for the development of fingerprints for the quality control of other herbal medicines.

Key words: HPLC fingerprint, HPLC fingerprint, Melilotus suaveolens Ledeb, Melilotus suaveolens Ledeb, Quality control, Quality control

中图分类号:

R284.1

Supporting: ^#Co-first author for the paper.
^*Corresponding author. Tel.: 86-27-63618208

郑国华^#, 王桂红^#, 吴木琴, 黄志军, 陶君彦^*, 张晓昱, 张琼光, 肖飞, 李崇明

草木樨药材指纹图谱研究

[J]. .

Guo-Hua Zheng^#, Gui-Hong Wang^#, Mu-Qin Wu, Zhi-Jun Huang, Jun-Yan Tao^*, Xiao-Yu Zhang, Qiong-Guang Zhang, Fei Xiao, Chong-Ming Li

Development of a fingerprint of Melilotus suaveolens Ledeb by high-performance liquid chromatography

[J]. .

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