酸枣仁和滇枣仁的HPLC指纹图谱分析及鉴别

doi:10.5246/jcps.2020.04.024

中国药学(英文版) ›› 2020, Vol. 29 ›› Issue (4): 252-259.DOI: 10.5246/jcps.2020.04.024

酸枣仁和滇枣仁的HPLC指纹图谱分析及鉴别

杨红帅¹, 刘秋怡¹, 殷卫东², 解满江¹, 吉翔¹, 邵双宇¹, 梁鸿¹, 屠鹏飞¹, 张庆英^1*

1. 北京大学医学部药学院天然药物及仿生药物国家重点实验室; 天然药物学系, 北京 100191
2. 淄博市临淄区人民医院药学部, 山东淄博 255400

收稿日期:2019-10-29 修回日期:2020-01-03 出版日期:2020-04-30 发布日期:2020-01-14
通讯作者: Tel./Fax: +86-10-82801725, E-mail: qyzhang@bjmu.edu.cn
基金资助:
National Project for Standardization of Chinese Material Medica (Grant No. ZYBZH-Y-JIN-34).

Identification and discrimination of Ziziphi Spinosae Semen and Ziziphi Mauritianae Semen based on HPLC fingerprint analysis

Hongshuai Yang¹, Qiuyi Liu¹, Weidong Yin², Manjiang Xie¹, Xiang Ji¹, Shuangyu Shao¹, Hong Liang¹, Pengfei Tu¹, Qingying Zhang^1*

1. State Key Laboratory of Natural and Biomimetic Drugs and Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
2. Department of Pharmacy, Linzi District People’s Hospital, Zibo 255400, China

Received:2019-10-29 Revised:2020-01-03 Online:2020-04-30 Published:2020-01-14
Contact: Tel./Fax: +86-10-82801725, E-mail: qyzhang@bjmu.edu.cn
Supported by:
National Project for Standardization of Chinese Material Medica (Grant No. ZYBZH-Y-JIN-34).

摘要/Abstract

摘要：

本文建立了一种简便、可靠、重现性好的酸枣仁和滇枣仁HPLC指纹图谱分析方法, 并对不同产地的酸枣仁和滇枣仁样品进行了分析。结果显示酸枣仁和滇枣仁各有12个共有指纹峰, 其中10个为酸枣仁和滇枣仁共有, 2个分别为酸枣仁和滇枣仁特有, 经对照品对照指认了其中5个共有指纹峰。以HPLC指纹图谱为导向, 对滇枣仁的两个特有指纹峰进行纯化分离和结构解析, 鉴定其结构分别为6′′′-芥子酰斯皮诺素和酸枣仁碱A。该HPLC指纹图谱分析方法能够快速、准确地区分和鉴别酸枣仁和滇枣仁, 可用于酸枣仁的质量控制。

关键词: 酸枣仁, 滇枣仁, 酸枣, 滇刺枣, HPLC指纹图谱

Abstract:

A simple, reproducible and reliable HPLC fingerprint analysis method of Ziziphi Spinosae Semen (ZSS) and Ziziphi Mauritianae Semen (ZMS) was established, and then it was applied to analyze samples collected from different locations. A total of 12 common fingerprint peaks were designated in ZSS and ZMS, respectively, and five of which were definitely identified. Of these common peaks, 10 peaks were presented in both ZSS and ZMS, whereas two were characteristic for ZSS and ZMS, respectively.Furthermore, the two characteristic peaks of ZMS were definitely assigned as 6′′′-sinapoylspinosin and frangufoline by HPLC guided purification and structural elucidation. The HPLC fingerprint analysis was proved to be useful in identifying and discriminating ZSS and ZMS, which was beneficial for quality control of ZSS and its products.

Key words: Ziziphi Spinosae Semen, Ziziphi Mauritianae Semen, Ziziphus jujuba var. spinosa, Ziziphi mauritianae, HPLC fingerprint

中图分类号:

R284

Supporting:

杨红帅, 刘秋怡, 殷卫东, 解满江, 吉翔, 邵双宇, 梁鸿, 屠鹏飞, 张庆英. 酸枣仁和滇枣仁的HPLC指纹图谱分析及鉴别[J]. 中国药学(英文版), 2020, 29(4): 252-259.

Hongshuai Yang, Qiuyi Liu, Weidong Yin, Manjiang Xie, Xiang Ji, Shuangyu Shao, Hong Liang, Pengfei Tu, Qingying Zhang. Identification and discrimination of Ziziphi Spinosae Semen and Ziziphi Mauritianae Semen based on HPLC fingerprint analysis[J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(4): 252-259.

参考文献

[1] Wu, P. Shennong Bencao Jing, Beijing: The Time Litera-ture and Art Press. 2008, 241.

[2] Chinese Pharmacopoeia Committee. Pharmacopoeia of the People’s Republic of China. Vol I, Beijing: Chinese Medical Science and Technology Press. 2015, 366-367.

[3] Cao, J.X.; Zhang, Q.Y.; Cui, S.Y.; Cui, X.Y.; Zhang, J.; Zhang, Y.H.; Bai, Y.J.; Zhao, Y.Y. Hypnotic effect of jujubosides from Semen Ziziphi Spinosae. J. Ethnopharmacol. 2010, 130, 163-166.

[4] Xie, J.B.; Zhang, Y.Q.; Wang, L.J.; Qi, W.Q.; Zhang, M.C. Composition of fatty oils from Semen Ziziphi Spinosae and its cardiotonic effect on isolated toad hearts. Nat. Prod. Res. 2012, 26, 479-483.

[5] Liu, Z.; Zhao, X.; Liu, B.; Liu, A.J.; Li, H.; Mao, X.; Wu, B.; Bi, H.S.; Jia, Y. Jujuboside A, a neuroprotective agent from semen Ziziphi Spinosae ameliorates behavioral disorders of the dementia mouse model induced by Aβ1-42. Eur. J. Pharmacol. 2010, 130, 163-166.

[6] Wang, L.E.; Cui, X.Y.; Cui, S.Y.; Cao, J.X.; Zhang, J.; Zhang, Y.H.; Zhang, Q.Y.; Bai, Y.J.; Zhao, Y.Y. Potentiating effect of spinosin, a C-glycoside flavonoid of Semen Ziziphi spinosae, on pentobarbital-induced sleep may be related to postsynaptic 5-HT1A receptors. Phytomedicine. 2010, 17, 404-409.

[7] Wang, L.F.; Huang, M.; Lu, X.Y.; Wei, R.Z.; Xu, J.Y. Ziziphi spinosae lily powder suspension in the treatment of depression-like behaviors in rats. BMC. Complem. Altern. M. 2017, 17, 238.

[8] Geng, X.; Li, Y.L. Progress on Chemical Composition and Pharmacological Activities of Semen Zizyphi Spinosae. Acta. Chin. Med. Pharm. 2016, 44, 84-86.

[9] Chen, B.Q.; Du, G.J.; Xu, Q.T. Sedative and hypnotic effect of jujubosides from Semen Ziziphi Spinosae. Chin. Med. Mat. 2002, 25, 429-430.

[10] Liu, A.P.; Ma, D.L.; Guo, L.X.; Wang, H.; Tan, Z.T.; Zheng, Y.G. Study on price fluctuation and influencing factors of Semen Zizyphi Spinosae. J. Agr. Tech. Serv. 2017, 34, 12-13.

[11] Bai, H.L.; Wang, J.; Liu, C.M.; Li, L. Isolation and Purification of Flavonoids from Ziziphus jujuba by High-Speed Counter-Current Chromatography. J. Chin. Chem. Soc. 2010, 57, 1071-1076.

[12] Cheng, G.; Bai, Y.J.; Zhao, Y.Y.; Tao, J.; Liu, Y.; Tu, G.Z.; Ma, L.B.; Liao, N.; Xu,X.J. Flavonoids from ziziphus jujuba mill var.spinosa. Tetrahedron. 2000, 56, 8915-8920.

[13] Han, B.H.; Park, M.H.; Han, Cyclic peptide and peptide alkaloids from seeds of zizyphus-vulgaris. Phytochemistry. 1990, 29, 3315-3319.

酸枣仁和滇枣仁的HPLC指纹图谱分析及鉴别

Identification and discrimination of Ziziphi Spinosae Semen and Ziziphi Mauritianae Semen based on HPLC fingerprint analysis

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics

本文评价

[1]	解满江, 王洁, 董晨光, 屠鹏飞, 张庆英. 枣仁安神胶囊的全药味薄层色谱鉴别[J]. 中国药学(英文版), 2022, 31(7): 499-506.
[2]	卢紫娟, 贾岩, 邢婕, 肖淑贤, 王玉龙, 秦文杰, 秦雪梅, 李震宇. 基于ITS/ITS2和HPLC指纹图谱技术的款冬花质量评价[J]. 中国药学(英文版), 2021, 30(1): 58-68.
[3]	林珠灿, 张龙, 张睿卓, 黄安玉, 郭素华. 基于炎症细胞模型的景天三七醋酸乙酯部位抗炎活性及其HPLC指纹图谱的研究[J]. 中国药学(英文版), 2015, 24(10): 647-653.
[4]	刘毅, 王伟, 赵玉英, 陈虎彪, 梁鸿, 张庆英. 红花岩黄芪药材的化学指纹分析[J]. 中国药学(英文版), 2015, 24(10): 654-659.
[5]	陈金凤, 屠鹏飞, 姜勇. 加压制备色谱技术结合HPLC指纹图谱导向快速分离制备红花提取物中的主要黄酮类成分[J]. 中国药学(英文版), 2014, 23(7): 490-495.
[6]	马晓庆, 邓伟峰, 杨东辉^*, 刘广学, 洪浩, 白光男, 蔡少青 . 秦艽生药中龙胆苦苷和落干酸的含量及其HPLC指纹图谱测定[J]. , 2010, 19(4): 243-250.