基于炎症细胞模型的景天三七醋酸乙酯部位抗炎活性及其HPLC指纹图谱的研究

doi:10.5246/jcps.2015.10.082

中国药学(英文版) ›› 2015, Vol. 24 ›› Issue (10): 647-653.DOI: 10.5246/jcps.2015.10.082

基于炎症细胞模型的景天三七醋酸乙酯部位抗炎活性及其HPLC指纹图谱的研究

林珠灿¹, 张龙¹, 张睿卓¹, 黄安玉¹, 郭素华^1,2*

1. 福建省中药学重点实验室, 福建中医药大学药学院, 福建福州 350122
2. 福建卫生职业技术学院, 福建福州 350101

收稿日期:2015-05-08 修回日期:2015-05-25 出版日期:2015-10-21 发布日期:2015-06-10
通讯作者: Tel.: 86-591-22861135, E-mail: guosuhua2005@126.com
基金资助:
Quality guarantee system of Chinese herbal medicines (Grant No. 201507002), Key project of science and technology of Fujian province (Grant No. 2014Y0053) and youth project of Fujian Provincial Health and Family Planning Commission (Grant No. 2015-1-77).

Anti-inflammatory effect of ethyl acetate extract of Sedum aizoon L. in LPS-stimulated RAW 264.7 macrophages and its HPLC fingerprint

Zhucan Lin¹, Long Zhang¹, Ruizhuo Zhang¹, Anyu Huang¹, Suhua Guo^1,2*

1. State Key Laboratory of Chinese Pharmacies, Pharmacy College, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
2. Fujian Health College, Fuzhou 350101, China

Received:2015-05-08 Revised:2015-05-25 Online:2015-10-21 Published:2015-06-10
Contact: Tel.: 86-591-22861135, E-mail: guosuhua2005@126.com
Supported by:
Quality guarantee system of Chinese herbal medicines (Grant No. 201507002), Key project of science and technology of Fujian province (Grant No. 2014Y0053) and youth project of Fujian Provincial Health and Family Planning Commission (Grant No. 2015-1-77).

摘要/Abstract

摘要：

景天三七广泛用于治疗各种出血、失眠、创伤肿痛, 但关于该药抗炎活性尚未见报道。本研究的目的是基于炎症细胞模型探讨景天三七提取物包括石油醚、醋酸乙酯及水部位的抗炎活性。结果显示, 富含酚酸和黄酮类成分的景天三七醋酸乙酯部位能显著抑制脂多糖诱导的RAW 264.7细胞中NO, TNF-α, IL-6的释放(P<0.01), 表明其醋酸乙酯部位具有潜在的抗炎活性, 同时建立该抗炎活性部位的HPLC指纹图谱。HPLC指纹图谱分析显示其抗炎活性可能与其所含的没食子酸、原儿茶酸、对羟基苯甲酸、没食子酸乙酯、鸢尾酚酮、5,7-二羟基色原酮、槲皮苷、槲皮素、木犀草素、山奈酚和异鼠李素等成分有关。综上, 景天三七醋酸乙酯部位体外具有抗炎活性, 是一潜在的天然抗炎剂。

关键词: 景天三七, 醋酸乙酯部位, RAW 264.7细胞, 炎症因子, HPLC指纹图谱

Abstract:

Sedum aizoon L. (SA) has been widely used for treatment of various hemorrhages, insomnia, pain and trauma; however, its anti-inflammatory activity is unknown. In this study, wefirstly investigated the anti-inflammatory activity of SA extracts by petroleum ether (PE), ethyl acetate (EtOAc), and H₂O in LPS-stimulated RAW 264.7 cells. Results showed that the EtOAc extract rich in phenolics and flavonoids significantly inhibited LPS-induced NO, TNF-α, and IL-6 production in RAW 264.7 cells (P<0.01), suggesting that this extract possessed potent anti-inflammatoryactivity. The phytochemical profile of the effective extract was subsequently analyzed by HPLC fingerprint with 11 standards. The results indicated that gallic acid, protocatechuic acid, p-hydroxybenzoic acid, ethyl gallate, iriflophene, 5,7-dihydroxy chromone, quercitrin, quercetin, luteolin, kaempferol, and isorhamnetin were present in this extract, which might contribute to its anti-inflammatory activity. Thus, the EtOAc extract of SA showed anti-inflammatory activity and could be used as a potential natural anti-inflammatory agent.

Key words: Sedum aizoon L., EtOAc extract, RAW 264.7 cell, Pro-inflammatory cytokines and mediators, HPLC fingerprint

中图分类号:

R931.5

Supporting:

林珠灿, 张龙, 张睿卓, 黄安玉, 郭素华. 基于炎症细胞模型的景天三七醋酸乙酯部位抗炎活性及其HPLC指纹图谱的研究[J]. 中国药学(英文版), 2015, 24(10): 647-653.

Zhucan Lin, Long Zhang, Ruizhuo Zhang, Anyu Huang, Suhua Guo. Anti-inflammatory effect of ethyl acetate extract of Sedum aizoon L. in LPS-stimulated RAW 264.7 macrophages and its HPLC fingerprint[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(10): 647-653.

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基于炎症细胞模型的景天三七醋酸乙酯部位抗炎活性及其HPLC指纹图谱的研究

Anti-inflammatory effect of ethyl acetate extract of Sedum aizoon L. in LPS-stimulated RAW 264.7 macrophages and its HPLC fingerprint

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