枣仁安神胶囊的全药味薄层色谱鉴别

doi:10.5246/jcps.2022.07.043

中国药学(英文版) ›› 2022, Vol. 31 ›› Issue (7): 499-506.DOI: 10.5246/jcps.2022.07.043

枣仁安神胶囊的全药味薄层色谱鉴别

解满江, 王洁, 董晨光, 屠鹏飞^*(), 张庆英^*()

北京大学医学部药学院天然药物及仿生药物国家重点实验室; 天然药物学系, 北京 100191

收稿日期:2021-09-11 修回日期:2021-11-21 接受日期:2021-11-28 出版日期:2022-07-31 发布日期:2022-07-31
通讯作者: 屠鹏飞, 张庆英
作者简介:
+ Tel.: +86-10-82805189, E-mail: pengfeitu@bjmu.edu.cn
+ Tel.: +86-10-82801725, E-mail: qyzhang@bjmu.edu.cn
基金资助:
National Key Research and Development Program of China (Grant No. 2018YFC1707300).

Simultaneous qualitative identification of all medicinal herbs in Zaoren Anshen capsule by thin-layer chromatography

Manjiang Xie, Jie Wang, Chenguang Dong, Pengfei Tu^*(), Qingying Zhang^*()

State Key Laboratory of Natural and Biomimetic Drugs and Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2021-09-11 Revised:2021-11-21 Accepted:2021-11-28 Online:2022-07-31 Published:2022-07-31
Contact: Pengfei Tu, Qingying Zhang

摘要/Abstract

摘要：

摘要

关键词: 枣仁安神胶囊, 酸枣仁, 丹参, 五味子, 薄层色谱法, 定性鉴别

Abstract:

The holistic characterization and quality control of all the medicinal herbs of proprietary Chinese medicines (PCMs) are of great significance to ensure their safety, efficacy, and consistency. Thin-layer chromatography (TLC), a simple and classic approach for qualitatively characterizing and examining quality markers of natural products, has been widely used in the characterization and quality control of traditional Chinese medicines. Zaoren Anshen (ZRAS) capsule, prepared from three medicinal herbs of fried Ziziphi Spinosae Semen, Salvia Miltiorrhiza Radix et Rhizoma, and vinegar-processed Schisandrae Chinensis Fructus, is a famous PCM in China for the treatment of insomnia, amnesia, and dizziness in clinical practice. However, no effective method is available so far for simultaneous identification and examination of all the three medicinal herbs of ZRAS capsule. In the present study, we developed a TLC method via twice-development and visualization by UV light or chromogenic agent, which could be used for simultaneous qualitative identification of all the three medicinal herbs of ZRAS capsule in one plate. Moreover, the sample preparation method was optimized. The developed TLC method was rapid, simple, low-cost, and effective, and thus it could be used for quality control of ZRAS capsule.

Key words: Zaoren Anshen capsule, Ziziphi Spinosae Semen, Salviae Miltiorrhizae Radix et Rhizoma, Schisandrae Chinensis Fructus, Thin-layer chromatography, Qualitative identification

Supporting:

解满江, 王洁, 董晨光, 屠鹏飞, 张庆英. 枣仁安神胶囊的全药味薄层色谱鉴别[J]. 中国药学(英文版), 2022, 31(7): 499-506.

Manjiang Xie, Jie Wang, Chenguang Dong, Pengfei Tu, Qingying Zhang. Simultaneous qualitative identification of all medicinal herbs in Zaoren Anshen capsule by thin-layer chromatography[J]. Journal of Chinese Pharmaceutical Sciences, 2022, 31(7): 499-506.

图/表 4

Figure 1. TLC chromatograms of different samples. A. Visualized under UV light at 254 nm. B. Colored and visualized immediately under visible light. C. Colored, heated, and visualized under visible light. 1. Mixed reference standards of jujuboside A (Ι), jujuboside B (II), and spinosin (III); 2. Reference standard extract of Ziziphi Spinosae Semen; 3. Mixed reference standards of salvianolic acid B (IV) and tanshinone IIA (VII); 4. Reference herb of Schisandrae Chinensis Fructus; 5. Mixed reference standards of schisandrol A (V) and schisandrin A (VI); 6. Reference herb of Schisandrae Chinensis Fructus; 7. Negative control sample without vinegar-processed Schisandrae Chinensis Fructus; 8. Negative control sample without Salviae Miltiorrhizae Radix et Rhizoma; 9. Negative control sample without fried Ziziphi Spinosae Semen; 10. ZRAS capsule sample (190702); 11. ZRAS capsule sample (190703); 12. ZRAS capsule sample (190704).

Figure 2. TLC chromatograms under different visualization conditions. (A) Visualized under UV light at 254 nm; (B) Visualized under UV light at 366 nm; (C) Colored and visualized immediately under visible light; (D) Colored, heated, and visualized under visible light. 1. Mixed reference standards of jujuboside A (Ι), jujuboside B (II) and spinosin (III); 2. Reference standard extract of Ziziphi Spinosae Semen; 3. Mixed reference standards of salvianolic acid B (IV) and tanshinone IIA (VII); 4. Reference herb of Schisandrae Chinensis Fructus; 5. Mixed reference standards of schisandrol A (V) and schisandrin A (VI); 6. Reference herb of Schisandrae Chinensis Fructus; 7. ZRAS capsule sample (190702); 8. ZRAS capsule sample (190703); 9. ZRAS capsule sample (190704).

Figure 3. TLC chromatograms on silica gel plates from different manufacturers. (A) silica gel 60 F254 plate from Merck Co.; (B) Silica gel 60 F254 plate from Qingdao Marine Chemical Co., Ltd.; (C) Silica gel 60 F254 plate from Yantai Chemical Industry Research Institute; (D) Silica gel 60 plate from Merck Co. 1. Mixed reference standards of jujuboside A (Ι), jujuboside B (II), and spinosin (III); 2. Reference standard extract of Ziziphi Spinosae Semen; 3. Mixed reference standards of salvianolic acid B (IV) and tanshinone IIA (VII); 4. Reference herb of Schisandrae Chinensis Fructus; 5. Mixed reference standards of schisandrol A (V) and schisandrin A (VI); 6. Reference herb of Schisandrae Chinensis Fructus; 7. ZRAS capsule sample (190702); 8. ZRAS capsule sample (190703); 9. ZRAS capsule sample (190704).

Figure 4. TLC chromatograms at different temperatures and humidities. 1. Mixed reference standards of jujuboside A (Ι), jujuboside B (II), and spinosin (III); 2. Mixed reference standards of salvianolic acid B (IV) and tanshinone IIA (VII); 3. Mixed reference standards of schisandrol A (V) and schisandrin A (VI); 4. ZRAS capsule sample (190702); 5. ZRAS capsule sample (190703); 6. ZRAS capsule sample (190704).

参考文献 7

[1]	Chen, B.W.; Yi, J.; Sun, B.; Jia, P.; Zhang, W.J.; Liu, B.Y. Efficacy and safety of Zaoren Anshen capsules in the treatment of insomnia: a meta-analysis of randomized controlled trials. Medicine. 2020, 99, e19033.
[2]	Chinese Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China. Vol I, Beijing: The China Medical Science Press. 2020, 1114–1115.
[3]	Xie, M.J.; Tu, P.F.; Zhang, Q.Y. Research advances of Zaoren Anshen prescription preparations. China J. Chin. Mater. Med. 2021, 46, 1301–1326
[4]	Li, D.Q.; Yue, D.D.; Liu, D.; Zhang, L.X.; Song, S.J. Phytochemical and chemotaxonomic study on Ziziphus jujuba mill. (Rhamnaceae). Biochem. Syst. Ecol. 2020, 91, 104058.
[5]	Wan, X.H.; Wang, Y.L.; Zhou, C.Z.; Guo, H.; Ma, S.; Wang, L. Resarch progress on chemical constituents and pharmacological effects of Salvia miltiorrhiza. Chin. Tradit. Herb. Drugs. 2020, 51, 788–798.
[6]	Liu, J.; Xu, J.; Guo, J.T. Review of active constituents and pharmacological activities of Schisandrae Chinensis Fructus [J]. Chin. J. Exp. Tradit. Med. Formulae. 2019, 25, 206–215.
[7]	Lu, H.S. Study on preparation technology and quality control of spine date seed anshen capsule. World J. Sleep Med. 2018, 5, 328–331.

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枣仁安神胶囊的全药味薄层色谱鉴别

Simultaneous qualitative identification of all medicinal herbs in Zaoren Anshen capsule by thin-layer chromatography

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