关键词: 改进的校正标准分离度积, 指纹图谱, 大豆异黄酮, 高效液相色谱-电喷雾质谱法

Abstract:

In this paper, we proposed a novel resolution criterion (improved calibrated normalized resolution product, r*') to evaluate separation quality of fingerprints. By comparing with the calibrated normalized resolution product (r*) and the hierarchical chromatographic response function (HCRF), the validity of this criterion was demonstrated by experimental chromatograms. The soy isoflavone extract was selected as the analytical object. The initial and end percentages of methanol and elution time affectinggradient elution were tested by orthogonal design. The final optimized conditions were as follows. It was detected by UV absorbance at 254 nm, column temperature was maintained at 36 ºC, solvent A was 0.1% (v/v) acetic acid, solvent B was methanol, gradient elution was from 34% to 65% B in a linear gradient in 25 min, and the flow-rate was set at 1.0 mL/min. In addition, the main ingredients of the soy isoflavone extract were confirmed by LC-ESI/MS.

Key words: Improved calibrated normalized resolution product, Fingerprints, Soy isoflavones, LC-ESI/MS