HPLC法快速测定当归、川芎中阿魏酸松柏酯的含量

HPLC法快速测定当归、川芎中阿魏酸松柏酯的含量

于玥, 张庆文, 王一涛, 李绍平^*

澳门大学中华医药研究院, 澳门

收稿日期:2007-03-15 修回日期:2007-08-10 出版日期:2007-09-15 发布日期:2007-09-15
通讯作者: 李绍平*

A rapid HPLC method for determination of coniferyl ferulate in Angelica sinensis and Ligusticum chuanxiong

Yue Yu, Qing-Wen Zhang, Yi-Tiao Wang, Shao-Ping Li^*

Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao SAR, China

Received:2007-03-15 Revised:2007-08-10 Online:2007-09-15 Published:2007-09-15
Contact: Shao-Ping Li*

摘要/Abstract

摘要：

目的建立一种快速可靠测定当归、川芎中活性成份阿魏酸松柏酯的HPLC方法。方法色谱条件: Zorbax ODS C18色谱柱 (250 mm × 4.6 mm ID, mm), 流动相采用1%醋酸与乙腈1:1等度洗脱, 检测波长318 nm。结果建立的HPLC方法日内和日间精密度分别为0.22% – 1.16%和0.86% – 2.62%, 阿魏酸松柏酯在0.380 – 0.038 mg·mL–1范围内线形关系良好 (r2 = 0.9995), 加样回收率为105.3%, RSD为2.7%。结论该方法能够准确、快速定量测定当归、川芎中阿魏酸松柏酯的含量,有利于提高对当归、川芎的质量控制。

关键词: 阿魏酸松柏酯, 阿魏酸松柏酯, 阿魏酸松柏酯, 当归, 当归, 当归, 川芎, 川芎, 川芎, HPLC, HPLC, HPLC

Abstract:

Aim A reliable and rapid HPLC method was developed for quantitative determination of coniferyl ferulate, an ester of ferulic acid, with multiple pharmacological activities in Angelica sinensis and Ligusticum chuanxiong, two commonly used Chinese medicines. Methods The determination was achieved by using a Zorbax ODS C18 analytical column (250 mm × 4.6 mm ID, 5 mm) at isocratic elution of 1% aqueous acetic acid and acetonitrile (1:1) with diode-array detection (318 nm). The calibration curve of coniferyl ferulate showed good linearity (r2 = 0.9995) within the test range. Results The developed method showed good precision with intra- and inter-day variations of 0.22% – 1.16% and 0.86% – 2.62% between the levels of 0.380 – 0.038 mg·mL–1, respectively. The repeatability represented as RSD of coniferyl ferulate was less than 2.7% for three levels (0.2 – 1.0 g of Angelica sinensis), and the recovery was 105.3% with RSD of 3.2%. Conclusion The validated method was successfully applied to quantify coniferyl ferulate in 12 samples of Danggui and Chuanxiong.

Key words: Coniferyl ferulate, Coniferyl ferulate, Angelica sinensis, Angelica sinensis, Ligusticum chuanxiong, Ligusticum chuanxiong, HPLC, HPLC

中图分类号:

R917.1

Supporting: Foundation item: Macao Science and Technology Development Fund (049/2005/A-R1) and Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Traditional Chinese Medicine (No.022021014003).
^*Corresponding author. Tel.: 853-3974692; fax: 853-28841358

于玥, 张庆文, 王一涛, 李绍平^*. HPLC法快速测定当归、川芎中阿魏酸松柏酯的含量[J]. .

Yue Yu, Qing-Wen Zhang, Yi-Tiao Wang, Shao-Ping Li^*. A rapid HPLC method for determination of coniferyl ferulate in Angelica sinensis and Ligusticum chuanxiong[J]. .

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