香港特有止泻中药材红党参的化学和分子生物学鉴定

香港特有止泻中药材红党参的化学和分子生物学鉴定

张艳波, 江仁望, 李松林, 乔春峰, 韩全斌, 徐宏喜, 黄家乐, 毕培曦^*, 邵鹏柱^*

1. 香港中文大学中医中药研究所;
2. 香港中文大学生物化学系;
3. 香港中文大学生物学系;
4. 香港赛马会中药研究院

收稿日期:2007-02-10 修回日期:2007-08-10 出版日期:2007-09-15 发布日期:2007-09-15
通讯作者: 毕培曦*, 邵鹏柱*

Chemical and molecular characterization of Hong Dangshen, a unique medicinal material for diarrhea in Hong Kong

Yan-Bo Zhang, Ren-Wang Jiang, Song-Lin Li, Chun-Feng Qiao, Quan-Bin Han,
Hong-Xi Xu, Ka-Lok Wong, Paul Pui-Hay But^*, Pang-Chui Shaw^*

1. Institute of Chinese Medicine;
2. Department of Biochemistry;
3. Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China;
4. Hong Kong Jockey Club Institute of Chinese Medicine, Hong Kong Science Park, Shatin, New Territories, Hong Kong, China

Received:2007-02-10 Revised:2007-08-10 Online:2007-09-15 Published:2007-09-15
Contact: Paul Pui-Hay But*, Pang-Chui Shaw*

摘要/Abstract

摘要：

目的鉴定香港特有止泻中药材红党参的基源植物和表面红色物质。方法将红党参的5S rRNA 基因间隔区序列和HPLC色谱指纹谱与正品党参5S rRNA 基因间隔区序列和HPLC色谱指纹谱进行比较研究; 将红党参表面红色物质的X-衍射光谱与中药赤石脂X-衍射光谱进行比较研究。结果红党参的HPLC色谱指纹谱和党参药材具有相同的特征色谱峰; 红党参的5S rRNA 基因间隔区序列与党参正品之一的素花党参(Codonopsis pilosula var. modesta)的5S rRNA基因间隔区序列最为相似; 红色物质的X-衍射光谱与中药赤石脂X-衍射光谱完全相同。结论红党参的基源是素花党参(Codonopsis pilosula var. modesta)的根, 红党参表面的红色物质是传统中药赤石脂(Halloysitum Rubrum)。红党参是素花党参根用中药赤石脂炮制而成, 科学名可定为Radix Codonopsis Praeparata Halloysita Rubra。

关键词: 红党参, 红党参, 红党参, 素花党参, 素花党参, 素花党参, 高效液相色谱, 高效液相色谱, 高效液相色谱, 5S rRNA基因间隔区序列, 5S rRNA基因间隔区序列, 5S rRNA基因间隔区序列, X-衍射, X-衍射, X-衍射, 赤石脂, 赤石脂, 赤石脂, 党参炔苷, 党参炔苷, 党参炔苷

Abstract: Aim To investigate the plant origin and the identity of the red substance on the surface of Hong Dangshen, a unique medicinal material for diarrhea in Hong Kong. Methods The HPLC fingerprints and 5S rRNA gene spacer sequences of Hong Dangshen were obtained and compared with those of genuine species of Radix Codonopsis. The X-ray diffraction spectrum of the red substance was analyzed and compared with that of Halloysitum Rubrum. Results Hong Dangshen showed very similar HPLC fingerprints to the Codonopsis species and the highest similarity to Codonopsis pilosula var. modesta in terms of 5S rRNA gene spacer sequences. The X-ray diffraction spectrum of the red substance was consistent with that of Halloysitum Rubrum. Conclusion The source plant of Hong Dangshen was suggested to be Codonopsis pilosula var. modesta, one of the genuine original plants of Radix Codonopsis (Dangshen) in the China Pharmacopoeia (2005 edition). The red substance on the surface of Hong Dangshen was indicated to be Halloysitum Rubrum, a traditional medicinal mineral for chronic diarrhea. Our data suggest that Hong Dangshen is derived from the roots of Codonopsis pilosula var. modesta which has been processed with Halloysitum Rubrum, and the name is suggested to be Radix Codonopsis Praeparata Halloysita Rubra.

Key words: Radix Codonopsis Praeparata Halloysita Rubra, Radix Codonopsis Praeparata Halloysita Rubra, Codonopsis pilosula var. modesta, Codonopsis pilosula var. modesta, HPLC, HPLC, 5S rRNA gene spacer, 5S rRNA gene spacer, X-ray, X-ray, Halloysitum Rubrum, Halloysitum Rubrum, lobetyolin, lobetyolin

中图分类号:

R284

Supporting: Foundation item: Hong Kong Jockey Club Charities Trust (Project No. HKJCICM-2-02R).
+Present address: School of Chinese Medicine, The University of Hong Kong, 10 Sassoon Road, Hong Kong, China
^*Corresponding authors. Tel.: 852-26096803; fax: 852-26035123

张艳波, 江仁望, 李松林, 乔春峰, 韩全斌, 徐宏喜, 黄家乐, 毕培曦^*, 邵鹏柱^*. 香港特有止泻中药材红党参的化学和分子生物学鉴定[J]. .

Yan-Bo Zhang, Ren-Wang Jiang, Song-Lin Li, Chun-Feng Qiao, Quan-Bin Han,
Hong-Xi Xu, Ka-Lok Wong, Paul Pui-Hay But^*, Pang-Chui Shaw^*. Chemical and molecular characterization of Hong Dangshen, a unique medicinal material for diarrhea in Hong Kong[J]. .

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