猴耳环的化学成分及其对T淋巴细胞增殖的影响

猴耳环的化学成分及其对T淋巴细胞增殖的影响

郭晓宇, 王乃利, 宝丽, 李轶华, 徐强, 姚新生^*

1.北京大学药学院天然药物学系, 北京 100083;
2.沈阳药科大学中药学院, 辽宁沈阳 110016;
3.深圳中药及天然药物研究中心, 广东深圳 518057;
4.南京大学生命科学学院医药生物技术国家重点实验室, 江苏南京 210093

收稿日期:2007-01-22 修回日期:2007-08-10 出版日期:2007-09-15 发布日期:2007-09-15
通讯作者: 姚新生*

Chemical constituents from Pithecellobium clypearia and their effects on T lymphocytes proliferation

Xiao-Yu Guo, Nai-Li Wang, Li Bo, Yi-Hua Li, Qiang Xu, Xin-Sheng Yao^*

1. Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China;
2. College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China;
3. Traditional Chinese Medicines and Natural Products Research Center Shenzhen, Shenzhen 518057, China;
4. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China

Received:2007-01-22 Revised:2007-08-10 Online:2007-09-15 Published:2007-09-15
Contact: Xin-Sheng Yao*

摘要/Abstract

摘要： 目的研究猴耳环Pithecellobium clypearia Benth枝叶的化学成分及其免疫活性。方法采用多种色谱学方法进行分离纯化, 利用波谱学方法进行结构鉴定; 并通过Con A诱导的T淋巴细胞增殖试验考察各化合物的免疫活性。结果从中分离并鉴定了8个化合物, 分别为(-)-表没食子儿茶素(1), (-)-5, 7, 3', 4', 5'-五羟基黄烷(2), (-)-表没食子儿茶素-7-没食子酸酯 (3), (-)-5, 3', 4', 5'-四羟基黄烷-7-没食子酸酯 (4), 槲皮素-3-O-α-L-吡喃鼠李糖苷(5), 杨梅树皮素-3-O-α-L-吡喃鼠李糖苷(6), 没食子酸 (7), 没食子酸乙酯 (8)。结论化合物3和8 为首次从该属植物中分离得到, 化合物1为首次从该种植物中分离得到; 化合物3能显著抑制Con A诱导的T淋巴细胞增殖, 其IC50为4.4 μmol·L–1。

关键词: 猴耳环, 猴耳环, 猴耳环, 黄酮类化合物, 黄酮类化合物, 黄酮类化合物, 黄烷, 黄烷, 黄烷, 淋巴细胞增殖, 淋巴细胞增殖, 淋巴细胞增殖

Abstract: Aim To investigate the chemical constituents from the twigs and leaves of Pithecellobium clypearia Benth and their immunomodulatory effects. Methods The constituents were separated and purified by various chromatographic methods and their structures were identified on the basis of spectral analysis. The immunomodulatory effects of all the compounds were examined by a Con A-induced T lymphocytes proliferation assay. Results Eight compounds were isolated and identified as (-)-epigallocatechin (1), (-)-5, 7, 3', 4', 5'-pentahydroxyflavan (2), (-)-epigallocatechin-7-gallate (3), (-)-5, 3', 4', 5'-tetrahydroxyflavan-7-gallate (4), quercitin-3-O-α-L-rhamnpyranoside (5), myricitin-3-O-α-L-rhamnpyranoside (6), gallic acid (7), and ethyl gallate (8), respectively. Conclusion Compounds 3 and 8 were isolated from this genus for the first time, and compound 1 was isolated from this species for the first time. Compound 3 exhibited a strong inhibition on the T lymphocytes proliferation induced by Con A with an IC50 of 4.4 μmol·L–1.

Key words: Pithecellobium clypearia, Pithecellobium clypearia, Flavonoids, Flavonoids, Flavane, Flavane, Lymphocytes proliferation, Lymphocytes proliferation

中图分类号:

R284.2

Supporting: ^*Corresponding author. Tel.: 86-20-85225849; fax: 86-20-85221559

郭晓宇, 王乃利, 宝丽, 李轶华, 徐强, 姚新生^*. 猴耳环的化学成分及其对T淋巴细胞增殖的影响[J]. .

Xiao-Yu Guo, Nai-Li Wang, Li Bo, Yi-Hua Li, Qiang Xu, Xin-Sheng Yao^*. Chemical constituents from Pithecellobium clypearia and their effects on T lymphocytes proliferation[J]. .

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