滇越金线兰醇提物对胰岛素抵抗的HepG2细胞降血糖活性及其化学成分的UPLC-MS/MS研究

doi:10.5246/jcps.2016.05.043

中国药学(英文版) ›› 2016, Vol. 25 ›› Issue (5): 380-386.DOI: 10.5246/jcps.2016.05.043

滇越金线兰醇提物对胰岛素抵抗的HepG2细胞降血糖活性及其化学成分的UPLC-MS/MS研究

蔡金艳^1*, 倪俊¹, 赵林²

1. 广东药科大学药学院, 广东广州 510006
2. 广东药科大学生命科学与生物制药学院, 广东广州 510006

收稿日期:2016-02-23 修回日期:2016-03-25 出版日期:2016-05-30 发布日期:2016-04-12
通讯作者: Tel.: +86-020-39352140, E-mail: caijy928@163.com
基金资助:
Pearl River Nova Program of Guangzhou 2015 (Grant No. 201506010061), Foundation for Distinguished Young Teachers in Higher Education of Guangdong (Grant No. YQ2015097), and National Natural Science Foundation of China (Grant No. 81001628).

An UPLC-MS/MS application to investigate the chemical composition of the ethanol extract from Anoectochilus chapaensis and its hypoglycemic activity in insulin-resistant HepG2 cells

Cai Jinyan^1*, Ni Jun¹, Zhao Lin²

1. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China;
2. School of Life Science and Bio-pharmaceutical, Guangdong Pharmaceutical University, Guangzhou 510006, China

Received:2016-02-23 Revised:2016-03-25 Online:2016-05-30 Published:2016-04-12
Contact: Tel.: +86-020-39352140, E-mail: caijy928@163.com
Supported by:
Pearl River Nova Program of Guangzhou 2015 (Grant No. 201506010061), Foundation for Distinguished Young Teachers in Higher Education of Guangdong (Grant No. YQ2015097), and National Natural Science Foundation of China (Grant No. 81001628).

摘要/Abstract

摘要：

民间俗称“药王”的滇越金线兰对治疗糖尿病有很好的疗效,但其活性成分的系统分析还未见报道。本研究将滇越金线兰粗提物作用于胰岛素抵抗的HepG2细胞,可显著促进细胞的葡萄糖摄取。应用超高效液相色谱串联质谱法(UPLC-MS/MS)快速分析滇越金线兰中的化学成分,通过保留时间、质谱精确分子量和特征碎片推定出63种成分,包括黄酮类、三萜和脂肪族化合物,结合胰岛素抵抗的HepG2对提取物进行降血糖活性筛选,采用UPLC-MS/MS快速进行成分分析,证明是一种可行且可靠的分离和鉴定中药中活性成分的方法。

关键词: 降血糖活性, HepG2细胞, 高效液相色谱串联质谱法, 滇越金线兰

Abstract:

Anoectochilus chapaensis Gagnep. (Orchidaceae) was named as the “king of medicine” because of its excellent efficacy for the treatment of diabetes. However, the bioactive constituents are unknown. An ethanol extract from A. chapaensis showed significant stimulating effect on glucose consumption in HepG2 cells. The chemical composition was investigated by UPLC-MS/MS in negative electrospray ionization (ESI) mode, and 63 compounds including flavonoids, triterpenoids, and aliphatic acids were tentatively identified by accurate mass and characteristic fragments. Moreover, the method of hypoglycemic screening with insulinresistant HepG2 cells and UPLC-MS/MS might be potentially useful in rapid and efficient characterization and primary prediction of natural products prior to traditional isolation.

Key words: UPLC-MS/MS, Hypoglycemic, HepG2 cells, Anoectochilus chapaensis

中图分类号:

R284

Supporting:

蔡金艳, 倪俊, 赵林. 滇越金线兰醇提物对胰岛素抵抗的HepG2细胞降血糖活性及其化学成分的UPLC-MS/MS研究[J]. 中国药学(英文版), 2016, 25(5): 380-386.

Cai Jinyan, Ni Jun, Zhao Lin. An UPLC-MS/MS application to investigate the chemical composition of the ethanol extract from Anoectochilus chapaensis and its hypoglycemic activity in insulin-resistant HepG2 cells[J]. Journal of Chinese Pharmaceutical Sciences, 2016, 25(5): 380-386.

[1]	戎怿, 黄碧云, 黄珺珺, 朱柳, 刘夏雯. 一种快速简单的超高效液相色谱串联四极杆质谱方法检测激素的葡糖醛酸化代谢产物[J]. 中国药学(英文版), 2016, 25(4): 291-301.
[2]	陈天洪, 蔡金艳, 倪俊, 杨帆. 阳荷醇提物降血糖活性及其化学成分的UPLC-MS/MS研究[J]. 中国药学(英文版), 2016, 25(2): 116-121.
[3]	蔡金艳, 赵林, 朱恩, 王成蹊. 滇越金线兰活性部位降血糖、增敏胰岛素和抗氧化活性研究[J]. 中国药学(英文版), 2014, 23(4): 262-267.
[4]	吴婷婷, 侯爱君, 洪臻怡, 朱依谆^*. 花椒提取物调节固醇和脂多糖诱导的胆固醇积聚[J]. , 2012, 21(6): 582-590.

滇越金线兰醇提物对胰岛素抵抗的HepG2细胞降血糖活性及其化学成分的UPLC-MS/MS研究

An UPLC-MS/MS application to investigate the chemical composition of the ethanol extract from Anoectochilus chapaensis and its hypoglycemic activity in insulin-resistant HepG2 cells

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