滇越金线兰活性部位降血糖、增敏胰岛素和抗氧化活性研究

doi:10.5246/jcps.2014.04.037

中国药学(英文版) ›› 2014, Vol. 23 ›› Issue (4): 262-267.DOI: 10.5246/jcps.2014.04.037

滇越金线兰活性部位降血糖、增敏胰岛素和抗氧化活性研究

蔡金艳^*, 赵林, 朱恩, 王成蹊

1. 广东药学院药科学院, 广东广州 510006
2. 广东药学院生命科学与生物制药学院, 广东广州 510006

收稿日期:2013-12-18 修回日期:2014-02-22 出版日期:2014-04-28 发布日期:2014-02-27
通讯作者: *Corresponding author. Tel.: +86-15920107845; Fax: +86-20-39352129; E-mail: caijy928@163.com
作者简介:*Corresponding author. Tel.: +86-15920107845; Fax: +86-20-39352129; E-mail: caijy928@163.com
基金资助:
National Natural Science Foundation of China (Grant No. 81001628) and Guangdong Natural Science Foundation (Grant No. S2013010014771).

Antihyperglycemic, insulin-sensitizing and antioxidant activities of the active fractions from Anoectochilus chapaensis

Jinyan Cai^*, Lin Zhao, En Zhu, Chengxi Wang

1. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China
2. School of Life Science and Bio-pharmaceutical, Guangdong Pharmaceutical University, Guangzhou 510006, China

Received:2013-12-18 Revised:2014-02-22 Online:2014-04-28 Published:2014-02-27
Contact: *Corresponding author. Tel.: +86-15920107845; Fax: +86-20-39352129; E-mail: caijy928@163.com
About author:*Corresponding author. Tel.: +86-15920107845; Fax: +86-20-39352129; E-mail: caijy928@163.com
Supported by:
National Natural Science Foundation of China (Grant No. 81001628) and Guangdong Natural Science Foundation (Grant No. S2013010014771).

摘要/Abstract

摘要：

采用高糖高脂饮食联合低剂量STZ诱导造成糖尿病大鼠模型, 实验组大鼠分别灌胃给予滇越金线兰总膏和溶剂萃取法所得各极性部位, 测定给药后大鼠血糖、胰岛素敏感性、抗氧化活性及NO水平等相关指标。胰腺切片, 进行病理学检查。与模型组相比, 乙酸乙酯部位给药组的随机血糖可见明显降低, 从给药前402.66至226.26 mg/dl (P<0.05), 而且该组大鼠的体重值明显高于模型对照组, 同时能提高胰岛素敏感性, 改善高糖负荷以后的糖耐量; 胰腺细胞形态较模型组有明显改善; 同时SOD活性增加, NO含量均显著降低。表明滇越金线兰的乙酸乙酯部位是其降血糖的主要活性部位, 其作用机制可能与改善胰岛素抵抗、增强机体抗氧化活性和降低血浆NO等机制有关。

关键词: 滇越金线兰, 降血糖, 胰岛素敏感指数, 活性部位

Abstract:

Anoectochilus chapaensisGagnep. (Orchidaceae), an indigenous and valuable Chinese folk medicine, has been widely used in China to treat diabetes. However, few reports are available about its constituents and activity. The present experiment was conducted to investigate the active fractions from A. chapaensis in diabetic rat model induced by high-fat diet plus streptozotocin. The total EtOH extract from the whole herbs of A. chapaensis,half of which was partitioned in sequence with petrol ether (PE), ethyl acetate (EtOAc), n-BuOH and H₂O, thus yielding four fractions, all of them were orally administered with an identical dose amount to 4 g/kg dried crude herbs once a day for consecutive two weeks to further investigate the antihyperglycemic activity. The EtOAc fraction caused a significant fall in the non-fasting blood glucose level of diabetic rats from (402.66±82.26) to (226.26±62.10) mg/dl, which may be attributed to ameliorating insulin resistance, modulating the activity of enzymatic antioxidants, reducing the content of NO, etc. Much more intact β cells in the islets of Langerhans in EtOAc fraction-treated groups than the negative control were observed, which greatly supported the morphological and functional elucidation. The OGTT evidencedthat EtOAc fraction could promote the endurance capacity of acute glucose increase in diabetic rats. The EtOAc fraction ofA. chapaensis contains some hypoglycemic and antioxidant principles with the potential to be developed further for the treatment of diabetes specifically associated with an insulin resistance state.

Key words: Anoectochilus chapaensis, Antihyperglycemic, ISI, Active fraction

中图分类号:

R962

Supporting: National Natural Science Foundation of China (Grant No. 81001628) and Guangdong Natural Science Foundation (Grant No. S2013010014771).

蔡金艳, 赵林, 朱恩, 王成蹊. 滇越金线兰活性部位降血糖、增敏胰岛素和抗氧化活性研究[J]. 中国药学(英文版), 2014, 23(4): 262-267.

Jinyan Cai, Lin Zhao, En Zhu, Chengxi Wang. Antihyperglycemic, insulin-sensitizing and antioxidant activities of the active fractions from Anoectochilus chapaensis[J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(4): 262-267.

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[2]	陈天洪, 蔡金艳, 倪俊, 杨帆. 阳荷醇提物降血糖活性及其化学成分的UPLC-MS/MS研究[J]. 中国药学(英文版), 2016, 25(2): 116-121.
[3]	吴正红, 平其能^*, 李建英, 蔡鹏. 壳聚糖和壳聚糖-EDTA轭合物双层包覆脂质体对胰岛素口服吸收的影响[J]. , 2006, 15(3): 139-146.
[4]	祁荣, 平其能^*, 徐瑞阳, 石勇平. 壳聚糖—半胱氨酸轭合物对胰岛素酶降解和降血糖作用的影响[J]. , 2004, 13(2): 124-129.

滇越金线兰活性部位降血糖、增敏胰岛素和抗氧化活性研究

Antihyperglycemic, insulin-sensitizing and antioxidant activities of the active fractions from Anoectochilus chapaensis

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