中药罗布麻叶中化学成分的LC/MS分析及其最佳采收期的研究

doi:10.5246/jcps.2018.04.026

中国药学(英文版) ›› 2018, Vol. 27 ›› Issue (4): 251-262.DOI: 10.5246/jcps.2018.04.026

中药罗布麻叶中化学成分的LC/MS分析及其最佳采收期的研究

仝令坤, 王弘, 张笑天, 郭宁宁, 韩雅, 王岱东, 陈世忠^*

北京大学医学部药学院, 北京 100191

收稿日期:2017-11-12 修回日期:2018-01-13 出版日期:2018-04-30 发布日期:2018-02-03
通讯作者: Tel.: +86-010-82802723, E-mail: chengshizhong66@163.com
基金资助:
Initiative Drug Discovery of National Science and Technology Major Project (Grant No. 2014ZX09304307-001-011).

Study on chemical constituents of Apocynum venetum L. leaves by LC/MS and determination of the best harvest season

Lingkun Tong, Hong Wang, Xiaotian Zhang, Ningning Guo, Ya Han, Daidong Wang, Shizhong Chen^*

School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2017-11-12 Revised:2018-01-13 Online:2018-04-30 Published:2018-02-03
Contact: Tel.: +86-010-82802723, E-mail: chengshizhong66@163.com
Supported by:
Initiative Drug Discovery of National Science and Technology Major Project (Grant No. 2014ZX09304307-001-011).

摘要/Abstract

摘要：

罗布麻叶是一种传统中药,具有平肝安神、清热利水的作用,现在被广泛用于高血压的治疗。准确的化学分析可以为罗布麻叶中化合物的含量测定及其最佳采收期的确定提供依据。本研究应用HPLC-DAD-ESI-IT-TOF技术对罗布麻叶指纹图谱中各色谱峰的化学成分进行了结构鉴定,同时,应用HPLC-DAD技术对24批罗布麻叶中的16个主要成分进行了含量测定,考察了不同采收期罗布麻叶的化合物含量变化规律。通过对罗布麻叶化学成分的解析,本研究共指认了30个化学成分,其中5种化合物为首次从罗布麻叶中指认。24批罗布麻叶中主要化合物含量变化规律表明, 5月份罗布麻叶中酚酸类、黄酮类化合物含量最高。因此,建议确定5月份为其最佳采收期。罗布麻叶最佳采收期的确定对提高罗布麻叶质量以及保证合理用药具有重要意义。

关键词: 罗布麻叶, 含量测定, 最佳采收期, HPLC-DAD-ESI-IT-TOF

Abstract:

The dried leaves of Apocynum venetum L. (AVL) is a traditional Chinese medicine widely used as antihypertensive medicine in China. Accurate determination of its best harvest season is important for its effective and safe use. In the present study, we developed a reliable method based on high-performance liquid chromatography-diode array detector-electro spray ionization-ion trap-time of flight (HPLC-DAD-ESI-IT-TOF) mass spectrometry and HPLC-DAD for the identification and quantification of major components in AVL leaves. Quantitative analysis of 24 samples collected weekly helped monitor the changes of compounds dynamically in AVL leaves to determine the best harvest season. A total of 30 compounds were identified, including quinic acid, five phenolic acids and 24 flavonoids. For the first time, 16 compounds were selected as marker compounds and simultaneously monitored weekly instead of monthly during the growth of the plant. The results showed that in May the leaves had the highest amount of phenolic acids, flavonoids and total compounds. Therefore, May should be the best harvest season for AVL leaves, which was distinct from previous studies. The established method was validated to be simple, accurate and precise, and thus it was of great importance for determination of the best harvest season.

Key words: Apocynum venetum L. leaves, Quantitative analysis, Best harvest season, HPLC-DAD-ESI-IT-TOF

中图分类号:

R284

Supporting:

仝令坤, 王弘, 张笑天, 郭宁宁, 韩雅, 王岱东, 陈世忠. 中药罗布麻叶中化学成分的LC/MS分析及其最佳采收期的研究[J]. 中国药学(英文版), 2018, 27(4): 251-262.

Lingkun Tong, Hong Wang, Xiaotian Zhang, Ningning Guo, Ya Han, Daidong Wang, Shizhong Chen. Study on chemical constituents of Apocynum venetum L. leaves by LC/MS and determination of the best harvest season[J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(4): 251-262.

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中药罗布麻叶中化学成分的LC/MS分析及其最佳采收期的研究

Study on chemical constituents of Apocynum venetum L. leaves by LC/MS and determination of the best harvest season

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