HPLC-DAD法同时测定六味五灵片中7种活性成分含量

doi:10.5246/jcps.2017.04.032

中国药学(英文版)

HPLC-DAD法同时测定六味五灵片中7种活性成分含量

程玲^1,2, 汪小涵^1,2, 申宝德¹, 徐平华¹, 沈成英¹, 郑娟^1,2, 廖魏波¹, 徐荣^1*, 袁海龙^1*

1. 空军总医院药学部, 北京 100142
2. 成都中医药大学药学院, 四川成都 611137

收稿日期:2016-11-13 修回日期:2017-02-13 出版日期:2017-04-26 发布日期:2017-03-08
通讯作者: Tel.: +86-010-66928505, E-mail: yhlpharm@126.com
基金资助:
State Project for Essential Drug Research and Development (Grant No. 2012ZX09J12108-04C) and Special Project of Military Health Ministry (Grant No. 13ZJZ12-5).

Simultaneous determination of seven bioactive constituents in Liuwei Wuling tablet by HPLC coupled with DAD

Ling Cheng^1,2, Xiaohan Wang^1,2, Baode Shen¹, Pinghua Xu¹, Chengying Sheng¹, Juan Zheng^1,2, Weibo Liao¹, Rong Xu^1*, Hailong Yuan^1*

1. Department of Pharmacy, Air Force General Hospital of PLA, Beijing 100142, China
2. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China

Received:2016-11-13 Revised:2017-02-13 Online:2017-04-26 Published:2017-03-08
Contact: Tel.: +86-010-66928505, E-mail: yhlpharm@126.com
Supported by:
State Project for Essential Drug Research and Development (Grant No. 2012ZX09J12108-04C) and Special Project of Military Health Ministry (Grant No. 13ZJZ12-5).

摘要/Abstract

摘要：

采用HPLC-DAD法,同时测定六味五灵片中连翘酯苷A,特女贞苷,连翘苷,五味子醇甲,五味子酯甲,五味子甲素和五味子乙素共7种有效成分的含量。采用Inertsil^® ODS-3色谱柱(250 mm×4.6 mm, 5 μm), 流动相为0.1%磷酸水(A)−乙腈(B),梯度洗脱,柱温25°C, 检测波长230 nm。7种化合物在相应的浓度范围内线性关系良好,相关系数均大于0.999。检测限与定量限分别在0.03−0.28 μg/mL和0.11−0.87 μg/mL之间。精密度、稳定性与重复性的RSD均小于2.34%,平均回收率均在96.71%−103.9%之间。建立的含量测定方法可有效对山东世博金都医药有限公司的十批样品进行检测,为六味五灵片的质量控制与下一步药理研究提供科学依据。

关键词: 多成分含量测定, 7种有效成分, 六味五灵片, HPLC-DAD

Abstract:

We employed high performance liquid chromatography (HPLC) method coupled with diode array detector (DAD) for the determinationof seven major bioactive compounds (forsythoside A, specnuezhenide, phillyrin, schisandrin, schisantherin A, deoxyschizandrin and schisandrin B) in Liuwei Wuling tablet (LWWLT). The HPLC baseline separation was carried out on an Inertsil^® ODS-3 column (250 mm×4.6 mm, 5 μm) at 25 °C with a gradient elution system composed of 0.1% phosphoric acid solution and acetonitrile. The detection wavelength was set at 230 nm. Calibration curves for seven compounds showed good linear regressions, with correlation coefficients exceeding 0.999 within the tested concentration range. The limits of detection and quantification of each compound were in the range of 0.03–0.28 μg/mL and 0.11–0.87 μg/mL, respectively. The relative standard deviation values of precision, stability and repeatability were less than 2.34%. The average recovery of all seven constituents ranged from 96.71% to 103.9%. The validated quantification method was successfully applied for the analysis of 10 batches of commercial LWWLT from Shandong Shibo Jindu Pharmaceutical Co., Ltd. These results would make foundations for quality control and the further pharmacology study of LWWLT.

Key words: Simultaneous determination, Seven bioactive constituents, Liuwei Wuling tablet, HPLC-DAD

中图分类号:

R917

Supporting:

程玲, 汪小涵, 申宝德, 徐平华, 沈成英, 郑娟, 廖魏波, 徐荣, 袁海龙. HPLC-DAD法同时测定六味五灵片中7种活性成分含量[J]. 中国药学(英文版), DOI: 10.5246/jcps.2017.04.032.

Ling Cheng, Xiaohan Wang, Baode Shen, Pinghua Xu, Chengying Sheng, Juan Zheng, Weibo Liao, Rong Xu, Hailong Yuan. Simultaneous determination of seven bioactive constituents in Liuwei Wuling tablet by HPLC coupled with DAD[J]. Journal of Chinese Pharmaceutical Sciences, DOI: 10.5246/jcps.2017.04.032.

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HPLC-DAD法同时测定六味五灵片中7种活性成分含量

Simultaneous determination of seven bioactive constituents in Liuwei Wuling tablet by HPLC coupled with DAD

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