HPLC-PDA 测定黄桷树叶提取液中绿原酸和芦丁的含量

doi:10.5246/jcps.2023.01.004

中国药学(英文版) ›› 2023, Vol. 32 ›› Issue (1): 45-51.DOI: 10.5246/jcps.2023.01.004

HPLC-PDA 测定黄桷树叶提取液中绿原酸和芦丁的含量

周燕青^#, 刘永红^#, 李艳婷, 陶彦如, 李进, 蒋心惠^*()

重庆医科大学药学院, 重庆市高校药物工程研究中心, 重庆 400016

收稿日期:2022-08-02 修回日期:2022-10-04 接受日期:2022-11-21 出版日期:2023-01-31 发布日期:2023-01-31
通讯作者: 蒋心惠
作者简介:
+ Tel.: +86-23-68485161, E-mail: Jiangxinhui@cqmu.edu.cn
基金资助:
Chongqing Medical University (Grant No. 400016).

Determination of chlorogenic acid and rutin in extractive solution of ficus virens leaves by HPLC-PDA

Yanqing Zhou^#, Yonghong Liu^#, Yanting Li, Yanru Tao, Jin Li, Xinhui Jiang^*()

Chongqing Research Center for Pharmaceutical Engineering, College of Pharmacy, Chongqing Medical University, Chongqing 400016, China

Received:2022-08-02 Revised:2022-10-04 Accepted:2022-11-21 Online:2023-01-31 Published:2023-01-31
Contact: Xinhui Jiang
About author:
# These authors contributed equally to this work.

摘要/Abstract

摘要：

建立测定黄桷树叶提取物中绿原酸和芦丁含量的HPLC-PDA法, 采用ODS2色谱柱, 梯度洗脱, 流动相甲醇–磷酸溶液(pH 3.0), 柱温25 ± 2 oC, 流速1.0 mL/min。通过质谱和紫外光谱技术对提取物中有效成分进行结构确证。经确证提取物中含有绿原酸和芦丁, 所建立的测定绿原酸和芦丁含量的HPLC-PDA法, 在所考察的浓度范围内线性关系良好, r ≥ 0.9998。定量限均小于0.06 μg/mL, 检测限均小于0.02 μg/mL; 相对回收率范围为99.10%–104.7%。以RSD值表示的日内精密度均小于1.89%, 日间精密度均小于1.82%。该方法灵敏度高, 准确度好, 专属性强, 操作简便, 可用于黄桷树叶提取液中绿原酸和芦丁的含量测定。经测定黄桷树叶提取液中芦丁和绿原酸含量分别为3.46和3.23 μg/mL。

关键词: 黄桷树叶, 绿原酸, 提取液, HPLC-PDA, 芦丁

Abstract:

A HPLC-PDA method was established for determining chlorogenic acid (CGA) and rutin (RU) in the extract of Pueraria lobata leaves. The ODS2 column was used with gradient elution, the mobile phase was methanol–phosphoric acid (AR) solution (pH 3.0), the column temperature was 25 ± 2 oC, and the flow rate was 1.0 mL/min. The effective components in the extract were identified by mass spectrometry and UV spectrometry. The results showed that the extraction contained CGA and RU. The HPLC-PDA method for determining CGA and RU showed an excellent linear relationship within the concentration range, r ≥ 0.9998. The results showed that the limits of quantitation and detection were all less than 0.06 and 0.02 μg/mL, respectively. The relative recoveries ranged from 99.10% to 104.7%. The intra-day and inter-day precisions expressed by RSD values were all less than 1.89% and 1.82%, respectively. The method was sensitive, specific, and easy to operate and could be used to determine CGA and RU in the extract of Pueraria lobata leaves. The contents of RU and CGA were 3.46 and 3.23 μg/mL, respectively.

Key words: Ficus virens leaves, Chlorogenic acid, Extractive solution of F. virens var. sublanceolata, HPLC-PDA, Rutin

Supporting:

周燕青, 刘永红, 李艳婷, 陶彦如, 李进, 蒋心惠. HPLC-PDA 测定黄桷树叶提取液中绿原酸和芦丁的含量[J]. 中国药学(英文版), 2023, 32(1): 45-51.

Yanqing Zhou, Yonghong Liu, Yanting Li, Yanru Tao, Jin Li, Xinhui Jiang. Determination of chlorogenic acid and rutin in extractive solution of ficus virens leaves by HPLC-PDA[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(1): 45-51.

图/表 4

Figure 1. The UV absorption spectrum of CGA (tR = 15.98 min, A), Peak 1 to be tested in the sample (tR = 16.01 min, B), RU (tR = 30.78 min, C), and Peak 2 to be tested in the sample (tR = 30.76 min, D).

Figure 2. The chromatogram of methanol (A), extract solution mixed with standard substance (B), standard substance solution, (C) extractive solution of FVS, and (D). 1. CGA; 2. RU.

Table 1. Calibration curves, LOD and LOQ measured for two analytes.

Table 2. Stability of CGA and RU in solution.

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HPLC-PDA 测定黄桷树叶提取液中绿原酸和芦丁的含量

Determination of chlorogenic acid and rutin in extractive solution of ficus virens leaves by HPLC-PDA

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