胆必清颗粒对急性胆管炎保护作用的机理研究

胆必清颗粒对急性胆管炎保护作用的机理研究

林秀珍^*, 公衍玲, 王宏波

1.天津医科大学药理教研室, 天津 300070;
2.山东即墨市人民医院普外科, 青岛 266200

收稿日期:2003-04-15 修回日期:2003-11-10 出版日期:2003-12-15 发布日期:2003-12-15
通讯作者: 林秀珍*

Mechanism Underlying Protective Effect of Danbiqing Granule on Experimental Acute Bacterial Cholangitis in Rabbits

LIN Xiu-zhen^*, GONG Yan-ling, WANG Hong-bo

1.Department of Pharmacology, Tianjin Medical University, Tianjin 300070, China;
2.Jimo People's Hospital, Qingdao 266000, China

Received:2003-04-15 Revised:2003-11-10 Online:2003-12-15 Published:2003-12-15
Contact: LIN Xiu-zhen*

摘要/Abstract

摘要： 目的探讨胆必清颗粒对实验性急性细菌性胆管炎保护作用的机理. 方法经胆管内注射1 mL大肠杆菌(1×108 cfu·mL)引发急性细菌性胆管炎. 采用硝酸还原法测定血清NO水平; 酸滴定法测定PLA2活性; 放免法测定血清TNF-α, IL6和血浆TXB2和PGF1α水平. 结果与对照组比较, 模型组血清NO、PL2、TNF和血浆TXB2水平均明显升高(P<0.05); 各胆必清治疗组的上述指标均显著降低(P<0.01). 结论胆必清颗粒能明显抑制前炎性因子PLA2和炎性细胞因子TNF-α, IL-6的过量产生,从而进一步揭示了该方剂的抗炎和屏障保护作用机理.

关键词: 急性胆管炎, 胆必清颗粒, NO, PLA2, TXB2, TNF-α

Abstract: Aim To study the mechanism of protective effects of Danbiqing granule (DBQ) on experimental acute bac-terial cholangitis in rabbits. Methods The acute bacterial cholangitis was induced by injecting 1 mL of 1 × 108 cfu·mL^-1 Escherchia coli suspension into common bile duit. The serum nitrous oxide (NO) levels were measured using nitric acid reductase kit. Phospholipase A2 (PLA2) activity was assayed by a method of acid titration (microassay). Serum tumor necrcsis factor-α (TNF-α), inferleukin-6 (IL-6) and plasma thromboxane B2 (TXB2), 6-keto-platelet growth factor 1 (PGF1α) were determined by radioimmunoassay. Results Compared with control group, serum NO, PLA2, TNF-α, IL-6and plasma TXB2 levels increased significantly in model group (P<0.01) while those of DBQ groups decreased significantly (P<0.01). Conclusion DBQ dramatically inhibits the overproduction of pro-inflammatory factor PLA2 and inflammatory cytokine. Hence, the mechanism of DBQ underlying anti-inflammatory and protective effect against acute bacterial cholangi-tis in rabbits has been revealed.

Key words: cholangitis, cholangitis, Danbiqing granule, Danbiqing granule, nitrous oxide, nitrous oxide, phospholipase A2, phospholipase A2, thromboxane B2, thromboxane B2, tumor necrcsis factor-α, tumor necrcsis factor-α

中图分类号:

R285

R965

Supporting: Foudation item: Supported by Tianjin Ninth-Five-Year Project, No. 983113411.
^*Corresponding author. Tel.: 022-23542523 (O)

林秀珍^*, 公衍玲, 王宏波. 胆必清颗粒对急性胆管炎保护作用的机理研究[J]. .

LIN Xiu-zhen^*, GONG Yan-ling, WANG Hong-bo . Mechanism Underlying Protective Effect of Danbiqing Granule on Experimental Acute Bacterial Cholangitis in Rabbits[J]. .

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