基于CeZnO纳米粒子的简单灵敏的电化学阻抗免疫传感器, 用于检测载脂蛋白E4

doi:10.5246/jcps.2021.03.019

中国药学(英文版) ›› 2021, Vol. 30 ›› Issue (3): 239-245.DOI: 10.5246/jcps.2021.03.019

基于CeZnO纳米粒子的简单灵敏的电化学阻抗免疫传感器, 用于检测载脂蛋白E4

张莹¹, 李聪¹, 刘一², 褚琳², 邹俊成¹, 鄢尤奇¹, 胡新¹, 凌笑梅¹^,^*()

1. 北京大学医学部药学院, 北京 100191
2. 北京大学人民医院, 北京 100044

收稿日期:2020-05-22 修回日期:2020-06-08 接受日期:2020-06-19 出版日期:2021-03-29 发布日期:2021-03-29
通讯作者: 凌笑梅
作者简介:
+ Tel.: +86-10-82801590, E-mail: lingxm@bjmu.edu.cn
基金资助:
The National Youth Foundation of China (Grant No. 81803487), National Natural Science Foundation (Grant No. 81673392), and China Postdoctoral Science Foundation (Grant No. 2018M631285).

A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle

Ying Zhang¹, Cong Li¹, Yi Liu², Lin Chu², Juncheng Zou¹, Youqi Yan¹, Xin Hu¹, Xiaomei Ling¹^,^*()

1 School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
2 People’s Hospital, Peking University, Beijing 100044, China

Received:2020-05-22 Revised:2020-06-08 Accepted:2020-06-19 Online:2021-03-29 Published:2021-03-29
Contact: Xiaomei Ling

摘要/Abstract

摘要：

载脂蛋白E4(APOE4)是迟发性阿尔茨海默症(AD)的重要危险因素。但是, APOE4的检测进展甚微, 现有的大多数检测方法耗时, 并且需要昂贵的仪器。本研究构建了基于富羧基的CeZnO纳米粒子的简单灵敏的电化学阻抗免疫传感器, 用于检测APOE4。在最佳条件下, 构建的免疫传感器对APOE4的线形范围为10到100 ng/mL, 检测极限为1.8 ng/mL (S/N = 3)。该传感器具有优异的选择性、重现性和稳定性, 可灵敏检测到人血清样品中的APOE4, 为APOE4的检测和AD的早期临床预防提供了有力的支持。

关键词: APOE4, CeZnO纳米粒子, 阿尔茨海默症, 无标记免疫传感器

Abstract:

Human apolipoprotein E4 (APOE4) is an important risk factor for late-onset Alzheimer’s disease (AD). However, little progress has been made for the detection of APOE4, and most of existing detection methods suffer from time-consuming process and expensive instruments. This study firstly proposed a simple and sensitive electrochemical method for detection of APOE4 based on carboxyl-rich CeZnO nanoparticles. Under the optimal conditions, the fabricated immunosensor exhibited a good linear relationship ranging from 10 to 100 ng/mL with the detection limit of 1.8 ng/mL (S/N = 3). The proposed electrochemical immunosensor had excellent selectivity, reproducibility and stability. Good performance was observed for sensitive determination of APOE4 in human serum sample, which provided a strong support for the detection of APOE4 and early clinical prevention of AD.

Key words: APOE4, CeZnO nanoparticle, Alzheimer’s disease, Label-free immunosensor

Supporting:

张莹, 李聪, 刘一, 褚琳, 邹俊成, 鄢尤奇, 胡新, 凌笑梅. 基于CeZnO纳米粒子的简单灵敏的电化学阻抗免疫传感器, 用于检测载脂蛋白E4[J]. 中国药学(英文版), 2021, 30(3): 239-245.

Ying Zhang, Cong Li, Yi Liu, Lin Chu, Juncheng Zou, Youqi Yan, Xin Hu, Xiaomei Ling. A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle[J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(3): 239-245.

图/表 3

Figure 1. (A) The SEM image and (B) higher magnification SEM image of CeZnO nanoparticle. (C) EIS and (D) CV responses of the electrode modification process.

Figure 2. Optimization of antibody incubation time: (A) Impedance value versus incubation time curve. Optimization of APOE4 incubation time: (B) Impedance value versus incubation time curve. The EIS response (C) and the calibration curves (D) of APOE4 electrochemical immunosensor after incubation with different concentrations of human APOE4 protein within the range of 10 to 100 ng/mL.

Table 1. Detection of APOE4 in human serum sample with proposed immunosensor.

参考文献 11

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基于CeZnO纳米粒子的简单灵敏的电化学阻抗免疫传感器, 用于检测载脂蛋白E4

A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle

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