胜红蓟内生真菌乙酸乙酯提取物的抗氧化及抑菌活性研究

doi:10.5246/jcps.2021.05.033

中国药学(英文版) ›› 2021, Vol. 30 ›› Issue (5): 421-433.DOI: 10.5246/jcps.2021.05.033

胜红蓟内生真菌乙酸乙酯提取物的抗氧化及抑菌活性研究

乔自鹏, 王奇志^*(), 刘建福, 王明元

华侨大学化工学院, 福建厦门 361021

收稿日期:2020-11-30 修回日期:2020-12-15 接受日期:2021-01-18 出版日期:2021-05-31 发布日期:2021-05-29
通讯作者: 王奇志
作者简介:
+ Tel.: +86-18738662596, E-mail: wqz@hqu.edu.cn
基金资助:
National Natural Science Foundation of China (Grant No. 31500162), the National Traditional Chinese Medicine Resources Survey Project for Public Health Service Subsidy for Traditional Chinese Medicine in 2018 (Grant No. 2018-43), the Natural Science?Foundation?of Fujian Province (Grant No. 2020J01078), Project for Postgraduates’ Innovative Fund in Scientific Research of Huaqiao University (Grant No. 18014087015).

Antioxidant and antibacterial activities of the ethyl acetate extracts of endophytic fungi isolated from Ageratum conyzoides L.

Zipeng Qiao, Qizhi Wang^*(), Jianfu Liu, Mingyuan Wang

College of Chemical Engineering, Huaqiao University, Fujian 361021, China

Received:2020-11-30 Revised:2020-12-15 Accepted:2021-01-18 Online:2021-05-31 Published:2021-05-29
Contact: Qizhi Wang

摘要/Abstract

摘要：

越来越多证据表明, 内生真菌代谢产物具有重要的生物学活性, 胜红蓟(Ageratum conyzoides)是菊科一种具有多种药理活性的入侵草本植物。我们从胜红蓟根、茎、叶和花中分离并鉴定内生真菌, 并对其乙酸乙酯提取物的体外抗氧化活性(DPPH、ABTS⁺和FRAP测定)、抑菌活性及黄酮、多酚含量进行测定。结果显示, 从胜红蓟中分离出共25株内生真菌, 基于ITS(Internal transcribed spacer)序列分析将其鉴定为14属, 其中以镰孢菌属Fusarium sp.(20%)为优势类群, 所有真菌提取物均表现出一定的抗氧化活性, 而菌株WZ07的提取物表现出较强的抗氧化活性以及较高的黄酮类和多酚含量。此外, 菌株WL01的提取物对革兰氏阳性和革兰氏阴性细菌均具有良好的抑菌活性。我们首次报道了胜红蓟内生真菌的抗氧化和抑菌活性, 为开发其药用活性成分奠定了基础。

关键词: 胜红蓟, 内生真菌, 抗氧化活性, 抑菌活性, 黄酮和多酚含量

Abstract:

Accumulating evidence has demonstrated that endophytic fungi metabolites possess important biological activities. Ageratum conyzoides L. (Asteraceae) is an invasive aromatic herb with immense therapeutic importance. In the present study, we aimed to isolate and identify endophytic fungi in the roots, stems, leaves and flowers of Ageratum conyzoides L. and evaluate its antioxidant activity (DPPH, ABTS⁺ and FRAP assays), the contents of flavonoids and phenolic and antibacterial activity of their ethyl acetate extracts. Accordingly, a total of 25 endophytic fungi were isolated from Ageratum conyzoides L. and classified into 14 genera based on the Internal transcribed spacer sequence, among which Fusarium sp. (20%) were found to be the dominant groups. All fungal extracts exhibited antioxidant activity, and the extracts of fungi WZ07 exhibited antioxidant activities as well as higher flavonoids and phenolic content in all tests. Moreover, the extracts of fungi WL01 had good antibacterial activity against both Gram-positive and Gram-negative bacteria. We, for the first time, reported the antioxidant and antibacterial activity of endophytic fungi from Ageratum conyzoides L., which may help lay the foundation for developing other medicinal components.

Key words: Ageratum conyzoides L., Endophytic fungi, Antioxidant activities, Antibacterial activity, Total flavonoids and polyphenols contents

Supporting:

乔自鹏, 王奇志, 刘建福, 王明元. 胜红蓟内生真菌乙酸乙酯提取物的抗氧化及抑菌活性研究[J]. 中国药学(英文版), 2021, 30(5): 421-433.

Zipeng Qiao, Qizhi Wang, Jianfu Liu, Mingyuan Wang. Antioxidant and antibacterial activities of the ethyl acetate extracts of endophytic fungi isolated from Ageratum conyzoides L.[J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(5): 421-433.

图/表 6

Table 1. Colonization site and identification results of endophytic fungi from Ageratum conyzoides L.

Figure 1. Phylogenetic tree analyses of endophytic fungi isolated from Ageratum conyzoides.

Table 2. The percentage yield of the ethyl acetate extracts obtained from fungi isolated in Ageratum conyzoides L.

Table 3. The total flavonoids and phenolic contents and IC50 value against DPPH, ABTS and Fe3+-reducing power of ethyl acetate extracts of 25 endophytic fungi.

Figure 2. Antioxidant activity of the ethyl acetate extracts of 25 endophytic fungi, and the positive control the vitamin C. (A) Efficiency of DPPH radical-scavenging; (B) Efficiency of ABTS radical-scavenging; (C) Reducing power of Fe3+.

Table 4. Antimicrobial activities of ethyl acetate crude extract from endophytic fungi in Ageratum conyzoides L.

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胜红蓟内生真菌乙酸乙酯提取物的抗氧化及抑菌活性研究

Antioxidant and antibacterial activities of the ethyl acetate extracts of endophytic fungi isolated from Ageratum conyzoides L.

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