绿茶提取物的体内抗氧化活性及对急性心肌梗死的保护作用

doi:10.5246/jcps.2013.04.049

绿茶提取物的体内抗氧化活性及对急性心肌梗死的保护作用

胜吕纶贵子, 魏博, 王莹, 李任强, 熊盛^*

1. 暨南大学基因工程药物国家工程中心广东省生物工程药物重点实验室, 广东广州 510632
2. 暨南大学生物医药研究院, 广东广州 510632
3. 暨南大学生命科学与技术学院生物工程系, 广东广州 510632

收稿日期:2012-10-30 修回日期:2012-11-26 出版日期:2013-07-10 发布日期:2013-07-10
通讯作者: 熊盛*

In vivo antioxidant activity and its protective effects of green tea extract
on acute myocardial infarction

Rinkiko Suguro, Bo Wei, Ying Wang, Renqiang Li, Sheng Xiong^*

1. Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, China
2. Biomedical R&D Center, Institute of Biomedicine, Jinan University, Guangzhou 510632, China
3. Department of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China

Received:2012-10-30 Revised:2012-11-26 Online:2013-07-10 Published:2013-07-10
Contact: Sheng Xiong*

摘要/Abstract

摘要：

应用大鼠急性心肌梗死模型研究中国龙井茶提取物的体内抗氧化活性及对急性心肌梗死的保护作用。收集大鼠血液、肝脏和心脏, 分别进行DNA损伤、肝脏抗氧化酶活性及其基因表达和心脏血管分布等多种检测, 以血管紧张素II的1型受体抑制剂, 诺沙坦作为阳性对照药物。与盐水处理组相比, 绿茶提取物组和诺沙坦处理组大鼠血液中某些DNA损伤产物的浓度明显降低, 肝脏抗氧化酶活性明显升高, 氧化酶基因的表达水平也明显升高; 形态学观察证实了绿茶提取物对急性心肌梗死的保护作用。绿茶提取物具有与诺沙坦类似的心肌保护活性, 这可能与它的清除自由基活性相关。

关键词: 心肌梗死, 绿茶提取物, 抗氧化活性, 心肌保护作用, 体内试验, 诺沙坦

Abstract:

The antioxidant activity and its protective effects of Green Tea Extract (GTE) from a Chinese green tea, Hangzhou Longjing, on acute myocardial infarction (AMI) were investigated in rats. Blood, liver and heart were collected to perform DNA damage test, hepatic antioxidant test, gene expression as well as capillary distribution study. A well-known angiotensin II type 1 receptor antagonist, Losartan was used as control. It was found there were significant reductions in certain DNA damage products' levels in GTE and Losartan treated groups compared to saline-treated group. In addition, significant increases in antioxidant enzyme activities and antioxidant gene expressions were observed in GTE and Losartan treated groups. Morphological study also demonstrated the cardioprotective effects of GTE on AMI. In conclusion, GTE has potent and similar cardioprotective effect as Losartan that may due to its ability to scavenge free radicals.

Key words: Myocardial infarction, Green Tea Extract, Antioxidant activity, Cardioprotective effects, In vivo, Losartan

中图分类号:

Supporting:

Foundation items: Ministry of Science and Technology of China (Grant No. 2012ZX09103-301-033), and the Fundamental Research Funds for the Central Universities (Grant No. 11611206).
^*Corresponding author. Tel./Fax: 86-20-85220504-311/309

胜吕纶贵子, 魏博, 王莹, 李任强, 熊盛^*. 绿茶提取物的体内抗氧化活性及对急性心肌梗死的保护作用[J]. , DOI: 10.5246/jcps.2013.04.049.

Rinkiko Suguro, Bo Wei, Ying Wang, Renqiang Li, Sheng Xiong^*. In vivo antioxidant activity and its protective effects of green tea extract
on acute myocardial infarction[J]. , DOI: 10.5246/jcps.2013.04.049.

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