芡实多糖理化性质及抗氧化活性研究

doi:10.5246/jcps.2014.08.075

中国药学(英文版)

芡实多糖理化性质及抗氧化活性研究

陈蓉¹, 薛满¹, 陈伟², 吴启南^3*

1. 苏州市食品药品检验所, 江苏苏州 215104
2. 苏州市中医医院, 江苏苏州 215009
3. 南京中医药大学江苏省中药方剂重点实验室, 江苏南京 210046

收稿日期:2014-02-17 修回日期:2014-05-13 出版日期:2014-08-31 发布日期:2014-05-20
通讯作者: Tel.: 86-25-85811507, Fax: 86-25-85811524
基金资助:
National Key Research and Development Program of the Ministry of Science and Technology of China during the “12th Five-Year Plan” (Grant No. 2011BAI04B06).

Evaluation of the physicochemical properties and antioxidant activities of polysaccharides from Semen Euryales

Rong Chen¹, Man Xue¹, Wei Chen², Qinan Wu^3*

1. Suzhou Institute for Food and Drug Control, Jiangsu Suzhou 215104, China
2. Suzhou Hospital of Traditional Chinese Medicine, Jiangsu Suzhou 215009, China
3. Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Jiangsu Nanjing 210046, China

Received:2014-02-17 Revised:2014-05-13 Online:2014-08-31 Published:2014-05-20
Contact: Tel.: 86-25-85811507, Fax: 86-25-85811524
Supported by:
National Key Research and Development Program of the Ministry of Science and Technology of China during the “12th Five-Year Plan” (Grant No. 2011BAI04B06).

摘要/Abstract

摘要：

采用水提醇沉、脱蛋白等工艺制备中药芡实多糖, 并通过纤维素柱和葡聚糖凝胶柱分离纯化。精多糖分子量为15 367 Da, 其单糖组成为葡萄糖:鼠李糖 (0.0142: 0.0026), 理化反应和红外光谱均符合多糖特征。体外抗氧化实验表明, 芡实多糖能够显著清除DPPH、超氧阴离子和过氧化氢等自由基, 并具有较强还原力。体内抗氧化实验显示, 芡实多糖能够显著提高D-半乳糖所致衰老小鼠的SOD、CAT、GSH-Px水平, 降低MDA水平。结果表明，芡实多糖具有广泛的研究前景。

关键词: 芡实, 多糖, 理化性质, 抗氧化活性

Abstract:

Polysaccharides were isolated from Semen Euryales by water extraction, alcohol precipitation and deproteinization. A refined polysaccharide, named EPJ, was separated into nine fractions by DEAE-52 cellulose chromatography and one of them was separated into two fractions by Sephadex G-100 column. The molecular weight of EPJ was determined to 15 367 Da and the monosaccharide composition was mainly glucose and rhamnose with the molar ratio of 5.46:1. The FT-IR spectra showed carbohydrate-related absorbance of polysaccharide functional groups. In vitro assay showed EPJ could significantly scavenge DPPH radical, superoxide anion, hydrogen peroxide and exhibited a strong reducing power. In vivo assay indicated that EPJ was effective in a D-galactose induced aging mice model. Results showed EPJ significantly inhibited formation of MDA and increased the activities of SOD, CAT, GSH-Px in a dose-dependent manner in mice tissues. The findings obtained from this study indicated that polysaccharides from Semen Euryales could be explored for a wide prospect of benefit.

Key words: Semen Euryales, Polysaccharide, Physicochemical properties, Antioxidant activities

中图分类号:

R284

Supporting:

陈蓉, 薛满, 陈伟, 吴启南. 芡实多糖理化性质及抗氧化活性研究[J]. 中国药学(英文版), DOI: 10.5246/jcps.2014.08.075.

Rong Chen, Man Xue, Wei Chen, Qinan Wu. Evaluation of the physicochemical properties and antioxidant activities of polysaccharides from Semen Euryales[J]. Journal of Chinese Pharmaceutical Sciences, DOI: 10.5246/jcps.2014.08.075.

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芡实多糖理化性质及抗氧化活性研究

Evaluation of the physicochemical properties and antioxidant activities of polysaccharides from Semen Euryales

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