黄芩苷抗氧化活性及对四氯化碳致大鼠肝损伤的保护作用

doi:10.5246/jcps.2015.08.068

中国药学(英文版) ›› 2015, Vol. 24 ›› Issue (8): 538-544.DOI: 10.5246/jcps.2015.08.068

黄芩苷抗氧化活性及对四氯化碳致大鼠肝损伤的保护作用

刘嵩¹, 吕晓慧^2,3, 孙宗喜^2,3*, 苏瑞强^2,3

1. 武汉科技大学医学院药学系, 湖北武汉 430062
2. 中药制药共性技术国家重点实验室, 山东临沂 276006
3. 鲁南制药集团股份有限公司, 山东临沂 276006

收稿日期:2015-03-16 修回日期:2015-04-23 出版日期:2015-08-22 发布日期:2015-05-12
通讯作者: Tel.: 86-539-8336639, Fax: 86-539-8336083, E-mail: zongxisun@163.com
基金资助:
National Key Basic Research and Development Program (Grant No. 2012CB724001), the Program of Hubei Provincial Department of Education (Grant No. Q20141105) and Research Program of Wuhan University of Science and Technology (Grant No. 2012ZX022).

Hepatoprotective and antioxidant effects of baicalin against CCl4-induced hepatotoxicity

Song Liu¹, Xiaohui Lv^2,3, Zongxi Sun^2,3*, Ruiqiang Su^2,3

1. Department of Pharmacology, College of Medicine, Wuhan University of Science and Technology, Wuhan 430062, China
2. State Key Laboratory of Generic Pharmaceutical Technology for Traditional Chinese Medicine, Linyi 276006, China
3. Lunan Pharmaceutical Group Co. Ltd., Linyi 276006, China

Received:2015-03-16 Revised:2015-04-23 Online:2015-08-22 Published:2015-05-12
Contact: Tel.: 86-539-8336639, Fax: 86-539-8336083, E-mail: zongxisun@163.com
Supported by:
National Key Basic Research and Development Program (Grant No. 2012CB724001), the Program of Hubei Provincial Department of Education (Grant No. Q20141105) and Research Program of Wuhan University of Science and Technology (Grant No. 2012ZX022).

摘要/Abstract

摘要：

黄芩在东亚地区尤其是我国有广泛的栽培。采用四氯化碳(CCl₄)致大鼠急性肝损伤模型研究黄芩苷的保肝作用。研究发现, 黄芩苷表现出较强的体外抗氧化活性。在CCl₄致大鼠急性肝损伤模型中, 给予黄芩苷可有效降低CCl₄引起的肝脏中谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酯酶(ALP), 总胆红素(TB)和丙二醛(MDA)含量的升高, 同时也提高了抗氧化防御物质超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽(GSH)的水平。切片病理观察结果也佐证了这一点。黄芩苷对CCl₄致大鼠氧化应激肝损伤有保护作用, 而这种保护机制可能与其清除自由基和抗氧化能力有关。

关键词: 黄芩苷, 保肝, 四氯化碳, 抗氧化

Abstract:

Scutellaria baicalensis is widely cultivated in eastern Asia, particularly in China. In the present study, we isolated baicalin from this plant and studied for its hepatoprotective activity against CCl₄-induced oxidative damage in rats. Our findings revealed that baicalin exhibited strong antioxidant activity in vitro. In established in vivo tests, baicalin showed effective protective effects by reducing the elevated levels of glutamate pyruvate transaminase(ALT), aspartate aminotransferase(AST), alkaline phosphatase (ALP), total bilirubin (TB) and malondialdehyde (MDA) against CCl₄-induced damage, and it restored the activities antioxidant defense substances, such as superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH), toward their normal levels. These data were supplemented with histopathological examination of rat liver sections. The results demonstrated that baicalin could be proposed to protect the liver against CCl₄-induced oxidative damage in rats, and the possible underlying mechanism of the activity could be due to its free radical-scavenging and antioxidant activity.

Key words: Baicalin, Hepatoprotective, CCl4, Antioxidant

中图分类号:

R284

Supporting:

刘嵩, 吕晓慧, 孙宗喜, 苏瑞强. 黄芩苷抗氧化活性及对四氯化碳致大鼠肝损伤的保护作用[J]. 中国药学(英文版), 2015, 24(8): 538-544.

Song Liu, Xiaohui Lv, Zongxi Sun, Ruiqiang Su. Hepatoprotective and antioxidant effects of baicalin against CCl4-induced hepatotoxicity[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(8): 538-544.

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黄芩苷抗氧化活性及对四氯化碳致大鼠肝损伤的保护作用

Hepatoprotective and antioxidant effects of baicalin against CCl4-induced hepatotoxicity

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