天麻内生真菌提取物抗氧化活性及天麻素的测定

doi:10.5246/jcps.2020.03.018

中国药学(英文版) ›› 2020, Vol. 29 ›› Issue (3): 206-213.DOI: 10.5246/jcps.2020.03.018

天麻内生真菌提取物抗氧化活性及天麻素的测定

梁晓霞^#,*, 熊城^#, 高迎迎, 范巧佳

四川农业大学动物医学院天然药物研究中心, 四川成都 611130

收稿日期:2019-11-15 修回日期:2019-12-04 出版日期:2020-03-30 发布日期:2020-01-08
通讯作者: Tel.: +86-28-86291470, E-mail: liangxiaoxia@sicau.edu.cn
基金资助:
The Foundation of Industrialization Demonstration and Promotion of Standardized Planting of Dominant Species of Gastrodia elata in Yingjing (General Service Station) and the Foundation of Sichuan Science and Technology Plan Project (Grant No. 2019YFS0170).

Antioxidantive activities and gastrodin determination of extracts of endophytic fungi isolated from Gastrodia elata Bl.

Xiaoxia Liang^#,*, Cheng Xiong^#, Yingying Gao, Qiaojia Fan

Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2019-11-15 Revised:2019-12-04 Online:2020-03-30 Published:2020-01-08
Contact: Tel.: +86-28-86291470, E-mail: liangxiaoxia@sicau.edu.cn
Supported by:
The Foundation of Industrialization Demonstration and Promotion of Standardized Planting of Dominant Species of Gastrodia elata in Yingjing (General Service Station) and the Foundation of Sichuan Science and Technology Plan Project (Grant No. 2019YFS0170).

摘要/Abstract

摘要：

天麻是一种治疗头痛眩晕的传统中药材。然而, 由于过度挖掘, 其野生资源紧缺。本研究从荥经天麻中分离到14株内生真菌, 对其中10株进行了鉴定, 分别属于新丛赤壳属、子囊菌属、镰刀菌、树粉孢属、聚生小穴壳菌。以总抗氧化能力、DPPH自由基清除能力为指标, 研究了10株内生真菌菌丝体醇提物抗氧化活性, 并测定其总多酚和总黄酮含量。结果表明, GER6 (树粉孢属)和GER2 (子囊菌属)显示出良好的体外抗氧化活性, 这可能与其酚类含量较高相关。但是在10株天麻内生真菌中仅GER6 (树粉孢属)中含有天麻素, 其含量为39.2 μg/g。综上可知, 天麻内生真菌GER6 (树粉孢属)有望开发成为一种天然抗氧化剂, 并有可能成为天麻替代品种。

关键词: 天麻, 内生真菌, 抗氧化活性, 天麻素

Abstract:

Gastrodia elata Bl. is a traditional Chinese herbal medicine with special pharmacological effects on headache and numbness. However, its wild sources are now in short supply because of over-excavation. A total of 14 endophytic fungi were isolated from Gastrodia elata Bl collected from Yinjing, in which 10 strains were identified, including Neonectria, Ascomycota, Fusarium, Oidiodendron and Dothiorella. All crude extracts showed some degrees of antioxidant activities by T-AOC method and DPPH methods, especially those from Oidiodendron GeR6 and Ascomycota sp. GeR2, which might be attributed due to their high phenolic contents. However, only Oidiodendron GeR6 contained gastrodin (39.2 μg/g) as the host plant in the crude extracts. In conclusion, the notable activities of GeR6 indicated its medical use as a natural antioxidant agent, as well as substitutable use for Gastrodia elata Bl. in the future.

Key words: Gastrodia elata Bl., Endophytic fungi, Antioxidantive activity, Gastrodin

中图分类号:

R284

Supporting:

The DNA sequence of endophytic fungi isolated from Gastrodia elata Bl.

梁晓霞, 熊城, 高迎迎, 范巧佳. 天麻内生真菌提取物抗氧化活性及天麻素的测定[J]. 中国药学(英文版), 2020, 29(3): 206-213.

Xiaoxia Liang, Cheng Xiong, Yingying Gao, Qiaojia Fan. Antioxidantive activities and gastrodin determination of extracts of endophytic fungi isolated from Gastrodia elata Bl.[J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(3): 206-213.

参考文献

[1] Wu, Z.Z.; Li, Y.S.; Liu, L.D.; Li, L.X.; Yang, Y. Study on the extraction technology and pharmacological function of active ingredient of Gastrodia elata Blume. Storage and Process. 2014, 14, 58-61.

[2] Liu, M.Q.; Luo, J.L. Research on extraction and purification of Gastrodia elata polysaccharides. China Pharm. 2011, 14, 1593-1596.

[3] Liyanapathirana, C.; Shahidi, F. Optimization of extraction of phenolic compounds from wheat using response surface methodology. Food Chem. 2005, 93, 47-56.

[4] Wang, L.; Zhang, X.G.; Li, X.Q. Studies on the main active ingredients and structure modification of Gastrodia elata. Hubei Agric. Sci. 2010, 5, 1146-1149.

[5] Luo, Z. .; Wu, J.Q.; Wu, M.C. The Antioxidative and antiviral activities of plant polysaccharide. J. Hubei Inst. Nat. 2007, 1, 77-81.

[6] Wang, L.P.; Zhou, S.M.; Dai, D.L.; Cao,J.S. Progress in plant phenolic compounds. Acta Agric. Zhejiangensis. 2010, 5, 696-701.

[7] Saikkonen, K.; Faeth, S.H.; Helander, M.; Sullivan, T.J. FUNGAL ENDOPHYTES: A continuum of interactions with host plants. Annu. Rev. Ecol. Syst. 1998, 29, 319-343.

[8] Stierle, A.; Strobel, G.; Stierle, D. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science. 1993, 260, 214-216.

[9] Devari, S.; Jaglan, S.; Kumar, M.; Deshidi, R.; Guru, S.; Bhushan, S.; Kushwaha, M.; Gupta, A.P.; Gandhi, S.G.; Sharma, J.P.; Taneja, S.C.; Vishwakarma, R.A.; Shah, B.A. Capsaicin production by Alternaria alternata, an endophytic fungus from Capsicum annum; LC-ESI-MS/MS analysis. Phytochemistry. 2014, 98, 183-189.

[10] Chithra, S.; Jasim, B.; Sachidanandan, P.; Jyothis, M.; Radhakrishnan, E.K. Piperine production by endophytic fungus Colletotrichum gloeosporioides isolated from Piper nigrum. Phytomedicine. 2014, 21, 534-540.

[11] Shweta, S.; Gurumurthy, B.R.; Ravikanth, G.; Ramanan, U.S.; Shivanna, M.B. Endophytic fungi from Miquelia dentata Bedd., produce the anti-cancer alkaloid, camptothecine. Phytomedicine. 2013, 20, 337-342.

[12] Mo, L.; Kang, J.C.; He, J.; Cao, J.J.; Su, H. A preliminary Study of the Composition of Endophytic Fungi from Gastrodia elata. J. Fungal Res. 2009, 6, 211-215.

[13] Tang, X.J.; Xing, G.S.; Li, F.; Ren, N. Symbiotic germination between gastrodia elata and fungal species of mycena. Mycosystema. 2001, 2001, 137-141.

[14] Fisher, P.J.; Petrini, O.; Sutton, B.C. A comparative study of fungal endophytes in leaves, xylem and bark of Eucalyptus in Australia and England. Sydowia. 1993, 45, 338-345.

[15] Taga, M.S.; Miller, E.E.; Pratt, D.E. Chia seeds as a source of natural lipid antioxidants. J. Am. Oil Chem. Soc. 1984, 61, 928-931.

[16] Li, Y.L.; Xin, X.M.; Chang, Z.Y.; Shi, R.J.; Miao, Z.M.; Ding, J.; Hao, G.P. The endophytic fungi of Salvia miltiorrhiza Bge.f. alba are a potential source of natural antioxidants. Bot. Stud. 2015, 56, 5.

[17] Hamilton, C.E.; Gundel, P.E.; Helander, M.; Saikkonen, K. Endophytic mediation of reactive oxygen species and antioxidant activity in plants: a review. Fungal Divers. 2012, 54, 1-10.

[18] Govindappa, M.; Bharath, N.; Shruthi, H.B.; Santoyo, G. In vitro antioxidant activity and phytochemical screening of endophytic extracts of crotalaria pallida. Free. Radic. Antioxid. 2011, 1, 79-86.

[19] Murthy, N.K.; Pushpalatha, K.C.; Joshi, C.G. Antioxidant activity and phytochemical analysis of endophytic fungi isolated from Lobelia nicotianifolia. J. Chem. Pharm. Res. 2011, 3, 218-225.

[20] Sadananda, T.S.; Nirupama, R.; Chaithra, K.; Govindappa, M.; Chandrappa, C.P.; Raghavendra, B.V. Antimicrobial and antioxidant activities of endophytes from Tabebuia argentea and identification of anticancer agent (lapachol). J. Med. Plants Res. 2011, 5, 3643-3652.

天麻内生真菌提取物抗氧化活性及天麻素的测定

Antioxidantive activities and gastrodin determination of extracts of endophytic fungi isolated from Gastrodia elata Bl.

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

本文评价

[1]	王涵, 李芳龙, 阿呷尔布, 梁晓霞, 更桑, 才让南迦. 西藏麻黄乙醇提取物及其不同极性组分的体外抗氧化活性研究[J]. 中国药学(英文版), 2023, 32(6): 478-486.
[2]	赵爱红, 王天霞, 刘晓艳, 赵伟红, 马建苹, 杨秀伟, 郭涛, 张友波. 竹叶椒抗炎内生真菌的分离[J]. 中国药学(英文版), 2021, 30(7): 570-577.
[3]	乔自鹏, 王奇志, 刘建福, 王明元. 胜红蓟内生真菌乙酸乙酯提取物的抗氧化及抑菌活性研究[J]. 中国药学(英文版), 2021, 30(5): 421-433.
[4]	刘继梅, 陈明华, 陈日道, 解可波, 陈大伟, 司书毅, 戴均贵. 内生真菌Periconia sp. F-31中的三个新化合物[J]. 中国药学(英文版), 2020, 29(4): 244-251.
[5]	宋爱伟, 吴鸿飞, 戴敏. 丹皮酚减缓动脉粥样硬化病变的形成及发展涉及脂质调节, 抗炎和抗氧化活性[J]. 中国药学(英文版), 2018, 27(8): 565-575.
[6]	陈楠, 梁晓霞. 紫花玉簪内生真菌分离鉴定及其抗氧化活性研究[J]. 中国药学(英文版), 2018, 27(10): 711-718.
[7]	杨登峰, 刘凤楼, 杨小龙. DNA甲基转移酶抑制剂改变小孢拟盘多毛孢次级代谢产物的研究[J]. 中国药学(英文版), 2017, 26(5): 355-359.
[8]	陈蓉, 薛满, 陈伟, 吴启南. 芡实多糖理化性质及抗氧化活性研究[J]. 中国药学(英文版), 2014, 23(8): 578-587.
[9]	胜吕纶贵子, 魏博, 王莹, 李任强, 熊盛^*. 绿茶提取物的体内抗氧化活性及对急性心肌梗死的保护作用[J]. , 2013, 22(4): 342-347.
[10]	张雪, 续洁琨, 王乃利, 栗原博, 姚新生^*. 金钗石斛中的菲类和木脂素类成分及其抗氧化作用 [J]. , 2008, 17(4): 314-318.
[11]	占纪勋, 郭洪祝, 戴均贵, 张元兴, 果德安^*. 刺囊毛霉对天麻素的生物转化研究[J]. , 2001, 10(4): 187-189.