关键词: 滇丹参, 丹参, 酚酸, HPLC, 含量测定

Abstract: A reverse-phase HPLC method was developed for the simultaneous separation and determination of five bioactive phenolic acids, yunnaneic acid E, rosmarinic acid, lithospermic acid, salvianolic acid B and salvianolic acid A in eight different samples of Salvia yunnanensis collected in Yunnan Province. For comparison, the sample of Salvia miltiorrhiza was included. All the samples were extracted for 60 min with 50% methanol in an ultrasonic bath. The optimal separation was achieved on a YMC-Pack Pro C18 column, with a gradient of 0.1% (v/v) phosphoric acid and acetonitrile, at a flow rate of 1.0 mL/min and at a detection wavelength of 280 nm. The separation was obtained within 65 min for five bioactive phenolic acids. All calibration curves showed good linearity (r²>0.999) within test ranges. The relative standard deviation of the method was less than 5% for intra- and inter-day assays. The mean recovery of the method was in the range from 97% to 104%, with RSD less than 5%. This assay was successfully applied to the quantitative determination of five bioactive phenolic acids in nine resource samples. The results showed that the developed HPLC assay was suitable for the quality control of S. yunnanensis and it can be used to differentiate S. yunnanensis from S. miltiorrhiza.

Key words: Salvia yunnanensis, Salvia miltiorrhiza, Phenolic acids, HPLC, Quantitative determination