UPLC-Q-TOF-MS/MS法测定不同产地天麻中6种成分的含量

doi:10.5246/jcps.2026.06.039

中国药学(英文版) ›› 2026, Vol. 35 ›› Issue (6): 552-559.DOI: 10.5246/jcps.2026.06.039

UPLC-Q-TOF-MS/MS法测定不同产地天麻中6种成分的含量

张丽藏¹, 张书菲², 解伟伟¹, 袁叶¹, 徐敬朴¹, 张玉倩¹, 李德强¹^,^*()

^1. 河北医科大学第二医院，河北石家庄 050000
^2. 河北医科大学药学院药物分析教研室，河北石家庄 050017

收稿日期:2026-03-06 修回日期:2026-03-28 接受日期:2026-04-12 出版日期:2026-07-05 发布日期:2026-07-05
通讯作者: 李德强

Determination of six components in Gastrodia elata from different origins by UPLC-Q-TOF-MS/MS

Licang Zhang¹, Shufei Zhang², Weiwei Xie¹, Ye Yuan¹, Jingpu Xu¹, Yuqian Zhang¹, Deqiang Li¹^,^*()

^1. Department of Clinical Pharmacy, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, China
^2. Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, Hebei, China

Received:2026-03-06 Revised:2026-03-28 Accepted:2026-04-12 Online:2026-07-05 Published:2026-07-05
Contact: Deqiang Li
Supported by:
The Natural Science Foundation of Hebei Province, China (Grant Nos. H2024206249 and H2023206050), and the Project of the Hebei Administration of Traditional Chinese Medicine (Grant No. 2025307).

摘要/Abstract

摘要：

本研究旨在通过建立UPLC-Q-TOF-MS/MS方法, 对道地药材云南天麻中的6种活性成分进行定性定量分析。采用超声提取法(30%甲醇)对天麻细粉中的6种化合物进行提取, 应用超高效液相色谱联用四极杆飞行时间质谱仪(UPLC-Q-TOF-MS/MS)进行分析。色谱条件: Atlantis T3 (2.1 mm × 150 mm, 3 μm, Waters)色谱柱, 0.1%甲酸水溶液(A)-乙腈(B)为流动相, 梯度洗脱, 柱温为40 ℃, 流速为0.5mL/min, 进样量为1 μL。负离子模式下, 采用多重反应监测模式进行检测, 考察了天麻素、巴利森苷A、巴利森苷B、巴利森苷C、巴利森苷E和对羟基苯甲醛的线性关系、检出限和定量限、精密度、回收率、稳定性和重复性。天麻中6种化合物的方法学考察结果良好。在线性范围内线性良好(r > 0.999), 平均回收率在96.83%−104.6%之间, 相对标准偏差(RSD)在0.25%−3.65%之间, 精密度检测的RSD值均在0.15%−2.33%之间。稳定性和重复性试验的RSD分别在1.00%−1.54%、0.70%−1.78%之间。此外, 云南、贵州、安徽三个产地的天麻中, 云南产地各成分含量较高。该研究建立的方法准确、重复性好、可操作性强, 可为天麻的质量评价与控制提供依据。

关键词: 天麻, 高脂血症, UPLC-Q-TOF-MS/MS, 含量测定, 质量评价

Abstract:

To establish arobust and reliable UPLC-Q-TOF-MS/MS–based method for the qualitative andquantitative determination of six active constituents in Gastrodiaelata Bl. from Yunnan, a well-recognizedgenuine medicinal herb, an ultrasound-assisted extraction and analyticalprocedure was developed and validated. Finely powdered samples of G. elata were extracted using 30% methanol under ultrasonic conditions,and the resulting extracts were analyzed by ultra-performance liquidchromatography coupled with quadrupole time-of-flight massspectrometry (UPLC-Q-TOF-MS/MS). Chromatographic separation was performed on anAtlantis T3 column (2.1 mm × 150 mm, 3 μm; Waters) using a gradient elutionprogram with 0.1% formic acid in water (A) and acetonitrile (B) as the mobilephases. The column temperature was maintained at 40 °C, with a flow rate of 0.5mL/min and an injectionvolume of 1 μL. Mass spectrometric detection was carried out in the negativeion mode using multiple reaction monitoring (MRM). Themethod was systematically validated by evaluating linearity, limits ofdetection and quantification, precision, accuracy (recovery), stability, and repeatability forgastrodin, parishin A, parishin B, parishin C, parishin E, and p-hydroxybenzaldehyde.The results demonstratedsatisfactory analytical performance for all six analytes. Excellent linearitywas achieved within the tested concentration ranges, withcorrelation coefficients (r) exceeding 0.999.Mean recoveries ranged from 96.83% to 104.6%, withrelative standard deviations (RSDs) between 0.25% and 3.65%. Intra- andinter-day precision values were within 0.15%–2.33%, while stability andrepeatability tests yielded RSDs of 1.00%–1.54% and 0.70%–1.78%, respectively. Furthermore,comparative analysis of samplescollected from Yunnan, Guizhou, and Anhui revealed that G. elata from Yunnan contained higher levelsof the six target constituents. Overall, the proposed method was accurate,reproducible, and practical, providing a dependableanalytical tool for the quality evaluation and quality control of G. elata.

Key words: Gastrodia, Hyperlipidemia, UPLC-Q-TOF-MS/MS, Content determination, Quality evaluation

Supporting:

张丽藏, 张书菲, 解伟伟, 袁叶, 徐敬朴, 张玉倩, 李德强. UPLC-Q-TOF-MS/MS法测定不同产地天麻中6种成分的含量[J]. 中国药学(英文版), 2026, 35(6): 552-559.

Licang Zhang, Shufei Zhang, Weiwei Xie, Ye Yuan, Jingpu Xu, Yuqian Zhang, Deqiang Li. Determination of six components in Gastrodia elata from different origins by UPLC-Q-TOF-MS/MS[J]. Journal of Chinese Pharmaceutical Sciences, 2026, 35(6): 552-559.

图/表 5

Table 1. Mass spectrum conditions of six components in G. elata.

Figure 1. Extracted ion chromatogram of G. elata and six components.

Table 2. Regression equations, correlation coefficients, linear ranges, LOD, and LOQ for the six constituents of G. elata.

Table 3. Recovery rates of six components in G. elata samples (n = 3).

Table 4. Contents of six components in G. elata samples (x ± s).

参考文献 19

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UPLC-Q-TOF-MS/MS法测定不同产地天麻中6种成分的含量

Determination of six components in Gastrodia elata from different origins by UPLC-Q-TOF-MS/MS

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