顶空-气相色谱-离子迁移谱法直接鉴定人工栽培和野生中药材(半枫荷)中的挥发性化合物

doi:10.5246/jcps.2023.05.033

中国药学(英文版) ›› 2023, Vol. 32 ›› Issue (5): 392-405.DOI: 10.5246/jcps.2023.05.033

顶空-气相色谱-离子迁移谱法直接鉴定人工栽培和野生中药材(半枫荷)中的挥发性化合物

葛永辉, 汪玲, 许粟, 姜天丽, 王金华^*()

贵阳学院食品与制药工程学院, 贵州贵阳 550025

收稿日期:2022-12-09 修回日期:2023-01-15 接受日期:2023-03-07 出版日期:2023-06-02 发布日期:2023-06-02
通讯作者: 王金华
作者简介:
+ Tel.: +86-15286007236, E-mail: wangjh963@126.com
基金资助:
The Guizhou Province Biological and Pharmaceutical Engineering Research Center (Grant No. QJHKY2019051), the Discipline and Master's Site Construction Project of Guiyang University by Guiyang City Financial Support Guiyang University (Grant No. SY-2020), and the Guizhou Science and Technology Support Program (Grant No. Qiankehe 2021-138).

Direct identification of volatile compounds in the artificially cultivated and wild Chinese medicinal materials (Semiliquidambar cathayesis) by headspace-gas chromatography-ion mobility spectrometry

Yonghui Ge, Ling Wang, Su Xu, Tianli Jiang, Jinhua Wang^*()

Food and Pharmaceutical Engineering Institute, Guiyang University, Guiyang 550025, Guizhou, China

Received:2022-12-09 Revised:2023-01-15 Accepted:2023-03-07 Online:2023-06-02 Published:2023-06-02
Contact: Jinhua Wang

摘要/Abstract

摘要：

半枫荷是一种传统的中药材, 其野生资源现已遭到严重破坏, 濒临灭绝。为了更好地保护和利用半枫荷资源, 人工栽培应运而生。本研究采用顶空-气相色谱-离子迁移谱法(HS-GC-IMS)结合主成分分析(PCA)对人工栽培和野生半枫荷中的挥发性有机化合物(VOCs)的变化进行分析。通过比较人工与野生品种半枫荷之间的差异来确定人工栽培半枫荷的药用价值。结果表明, 半枫荷人工栽培和野生品种的VOCs组成相同, 用于调节其药效的皮和茎中的挥发性有机化合物没有显着差异。该研究为人工栽培半枫荷的应用和野生品种的替代提供了科学理论。

关键词: 半枫荷, 中药, 指纹, 挥发性有机化合物, 顶空-气相色谱-离子迁移谱法

Abstract:

Semiliquidambar cathayesis (SC) is often used as traditional Chinese medicine (TCM) in the market, and its wild resources are now severely damaged and on the verge of extinction. In order to preserve and apply SC resources preferably, artificially cultivated SC is generated. In the present study, the variations of volatile organic compounds (VOCs) in the artificially cultivated and wild SC were analyzed by headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) combined with principal component analysis (PCA). The medicinal value of artificially cultivated SC was determined by comparing the differences between these SCs. The results showed that the artificially cultivated and wild SCs had the same composition for the VOCs, and the VOCs in the bark and stem used to adjust the efficacy of liquid medicine in SCs were not significantly different. Collectively, the findings provided a scientific theory for the application of artificially cultivated SC and the replacement of wild SC.

Key words: Semiliquidambar cathayesis, Traditional Chinese medicine, Fingerprint, Volatile organic compounds, Headspace-gas chromatography-ion mobility spectrometry

Supporting:

葛永辉, 汪玲, 许粟, 姜天丽, 王金华. 顶空-气相色谱-离子迁移谱法直接鉴定人工栽培和野生中药材(半枫荷)中的挥发性化合物[J]. 中国药学(英文版), 2023, 32(5): 392-405.

Yonghui Ge, Ling Wang, Su Xu, Tianli Jiang, Jinhua Wang. Direct identification of volatile compounds in the artificially cultivated and wild Chinese medicinal materials (Semiliquidambar cathayesis) by headspace-gas chromatography-ion mobility spectrometry[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(5): 392-405.

图/表 6

Figure 1. 3D-topographic in different samples. RIP represents the reactant ion peak.

Figure 2. Reporter analysis of the artificially cultivated and wild SC samples. (A) The topographic plot of GC-IMS spectra; (B) The spectrum of the first sample of each group in the topographic plot was selected as the reference, and the spectrum was obtained by subtracting the reference from the spectra of other samples. RIP represents the reactant ion peak.

Figure 3. The topographic plot of GC-IMS spectra with the selected markers obtained for SC. RIP represents the reactant ion peak.

Table 1. GC-IMS integration parameters of VOCs in SC.

Figure 4. Gallery plot of the selected signal peak areas obtained with different parts of the artificially cultivated and wild SCs. 1-1, 1-2, 1-3, and 1-4 represent the leaf, branch, bark, and stem of the artificially cultivated SC, respectively; 2-1, 2-2, 2-3, and 2-4 represent the leaf, branch, bark, and stem of the wild SC, respectively.

Figure 5. PCA results of different parts of the artificially cultivated and wild SCs. A represents the PCA result of the artificially cultivated SC; B represents the PCA result of the wild SC; C represents the sum of the PCA results of the artificially cultivated and wild SCs; a–d represent the the leaf, branch, bark, and stem of the artificially cultivated SC, respectively; e–h represent the leaf, branch, bark, and stem of the wild SC, respectively.

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顶空-气相色谱-离子迁移谱法直接鉴定人工栽培和野生中药材(半枫荷)中的挥发性化合物

Direct identification of volatile compounds in the artificially cultivated and wild Chinese medicinal materials (Semiliquidambar cathayesis) by headspace-gas chromatography-ion mobility spectrometry

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