姜黄素通过miR-29b/KDM2A介导PI3K/AKT信号通路抑制宫颈癌细胞的侵袭和迁移

doi:10.5246/jcps.2020.06.038

摘要/Abstract

摘要：

研究姜黄素通过调控miR-29b/KDM2A抑制宫颈癌细胞侵袭和迁移的作用机制。用姜黄素处理HeLa宫颈癌细胞, 观察细胞活力、细胞迁移和细胞侵袭情况。我们还评估了microRNA-29b (miR-29b)对HeLa SiHa细胞生物学行为的影响。利用荧光素酶报告基因实验预测和确认miR-29b的潜在靶基因,分析姜黄素和miR-29b对PI3K/AKT信号通路的影响。姜黄素对HeLa细胞的增殖、迁移和侵袭均有抑制作用(P<0.05)。在HeLa细胞中,姜黄素可促进miR-29b的表达(P<0.01), 而miR-29b的下调可恢复姜黄素对HeLa细胞增殖、迁移和侵袭的影响(P<0.05)。KDM2A被证实是miR-29b的直接靶基因, 而姜黄素和miR-29b可以调控PI3K/AKT信号通路的活性(P<0.05)。姜黄素对宫颈癌具有有效的保护作用。姜黄素可通过miR-29b/KDM2A/PI3K/AKT通路抑制宫颈癌细胞的增殖、迁移和侵袭。

关键词: 姜黄素, 扩散, 迁移, 入侵, MiR-29b, 宫颈癌

Abstract:

In this study, we aimed to examine whether curcumin exerted its anti-tumor effects by regulating miR-29b/KDM2A in cervical cancer cells. The cell viability, migration and invasion were estimated in HeLa cervical cancer cells treated with curcumin. The effects of microRNA-29b (miR-29b) on biological behaviors of HeLa SiHa cells were also assessed. Potential target genes of miR-29b were predicted and confirmed using a luciferase reporter assay, and the effects of curcumin and miR-29b on the PI3K/AKTsignaling pathway were analyzed. Curcumin treatment inhibited cell proliferation, migration and invasion of HeLa cells (P<0.05). The miR-29b expression was promoted by curcumin treatment in HeLa cells (P<0.01), and miR-29b depletion could restore the effects of curcumin on cell proliferation, migration and invasion of HeLa cells (P<0.05). KDM2A was proved as a direct target gene of miR-29b, and the activity of the PI3K/AKT signaling could be regulated by curcumin and miR-29b (P<0.05). All the data revealed that curcumin played a protective role in cervical cancer. The proliferation, migration and invasion of cervical cancer cells were inhibited by curcumin through the miR-29b/KDM2A/PI3K/AKT pathway.

Key words: Curcumin, Proliferation, Migration, Invasion, MiR-29b, Cervical cancer

中图分类号:

R965

Supporting:

郭锡春, 陈景华, 高如俊, 韩秀媛. 姜黄素通过miR-29b/KDM2A介导PI3K/AKT信号通路抑制宫颈癌细胞的侵袭和迁移[J]. 中国药学(英文版), 2020, 29(6): 398-410.

Xichun Guo, Jinghua Chen, Rujun Gao, Xiuyuan Han. Curcumin inhibits cervical cancer cell proliferation, migration and invasion by suppressing the PI3K/AKT signaling pathway via the miR-29b/KDM2A axis[J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(6): 398-410.

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