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中国药学(英文版)

• 【“现代仪器技术在药物研究中的应用”系列综述】 • 上一篇    下一篇

多色免疫组化/荧光联合多光谱成像技术在肿瘤免疫治疗领域的应用

李文哲*, 袁霞, 徐波, 宋书香   

  1. 北京大学药学院 天然药物及仿生药物国家重点实验室, 北京 100191
  • 收稿日期:2020-09-05 修回日期:2020-09-27 出版日期:2020-10-31 发布日期:2020-09-28
  • 通讯作者: Tel: +86-10-82805217, E-mail: 1916193009@bjmu.edu.cn

Applications of multiplexed immunohistochemistry/immunofluorescence and multispectral imaging technology in the field of tumor immunotherapy

Wenzhe Li*, Xia Yuan, Bo Xu, Shuxiang Song   

  1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
  • Received:2020-09-05 Revised:2020-09-27 Online:2020-10-31 Published:2020-09-28
  • Contact: Tel: +86-10-82805217, E-mail: 1916193009@bjmu.edu.cn

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摘要：

多色免疫组化/荧光结合多光谱成像技术是一种创新型的多靶标病理组织染色、成像及分析技术。通过在一张组织切片上原位检测多种生物标志物的表达水平及空间信息,并配合定量分析软件,可对细胞的表型、丰度、形态及细胞间相互关系作出分析,并给出具有统计学意义的细胞组学数据。近年来,随着多色免疫组化/荧光技术的快速发展及多光谱成像技术的日臻成熟,该技术被成功地应用于肿瘤免疫研究领域,主要包括：肿瘤免疫微环境研究、免疫治疗药物研发、预后和转移风险评估等。基于以上现状,本综述对多色免疫组化/荧光结合多光谱成像技术的原理、工作流程、优缺点进行了阐述,并重点讨论了近年来该技术在抗肿瘤免疫治疗药物开发及肿瘤微环境研究领域的应用。

关键词: 多色免疫组化/荧光, 多光谱成像技术, 肿瘤微环境, 肿瘤免疫治疗

Abstract:

Multiplexed immunohistochemistry/fluorescence (mIHC/IF) in combination with multispectral unmixing is a novelmultitarget histopathological staining and imaging technique. By simultaneously revealing expression level and spatial information for up to eight biomarkers in situ, in addition to a nuclear stain within a single formalin-fixed paraffin-embedded (FFPE) tissue section, this technology can analyze the phenotype, abundance, morphology and intercellular relationship of cells while providing statistically significant results. In recent years, technical improvements have brought new insight into mIHC/IF and multispectral imaging approaches, which have been successfully applied in the field of cancer immunotherapy, specifically in regard to tumor microenvironment research, immunotherapy drug discovery, and prognostic and metastatic risk evaluation. This reviewhighlights the principle, workflow, advantages and disadvantages of the technology, and discusses the latest applications of mIHC/IF-based imaging technology in the field ofTME-related research and immunotherapy drug discovery. 

Key words: Multiplexed immunohistochemistry/immunofluorescence (mIHC/IF), Multispectral imaging, Tumor microenvironment, Tumor immunotherapy

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