表面辅助激光解吸/离子化质谱技术用于药物和代谢物分析

doi:10.5246/jcps.2020.08.054

摘要/Abstract

摘要：

表面辅助激光解吸/离子化质谱(SALDI-MS)技术是利用无机纳米材料为基质, 以促进待测物的解吸附和离子化。对比传统的基质辅助激光解吸/离子化质谱(MALDI-MS)技术, SALDI-MS具有质谱信号在低质量端干扰小、重现性好、耐盐度高等优点, 非常适合于小分子化合物的分析。近年来, 研究人员开发了一系列纳米材料, 成功地将该技术应用于小分子药物和代谢物分析领域, 包括药物筛查和定量、药物递送、代谢物轮廓分析、生物标志物发现等。本综述总结了最新发展的SALDI-MS基质材料(金属类材料、碳材料、硅材料和有机框架纳米材料)在这个领域的应用。此外, 文中对SALDI-MS技术进行了展望, 以期能进一步促进该技术的发展。

关键词: 激光解吸/离子化质谱, 药物分析, 代谢物检测, 纳米材料

Abstract:

Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) uses inorganic nanomaterials as matrixes to facilitate desorption and ionization of analytes. Compared with the traditional matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) technique, SALDI-MS has the advantages of less interference in the low mass range, better reproducibility and higher salt tolerance. It is highly suitable for the analysis of small molecule compounds. In recent years, researchers have developed a range of nanomaterials that are successfully applied to the field of small molecule drug and metaboliteanalysis including drug screening and quantification, drug delivery, metabolite profiling, biomarker discovery and so forth. This review summarizes the latest progress of SALDI-MS matrix materials such as metal-based, carbon-based, silica-based nanomaterials and organic framework nanomaterials and their applications. In addition, our perspective of SALDI-MS technology is also discussed for further advancement.

Key words: Laser desorption/ionization mass spectrometry, Drug analysis, Metabolite detection, Nanomaterial

中图分类号:

R917

Supporting:

马雯, 宋书香, 李军. 表面辅助激光解吸/离子化质谱技术用于药物和代谢物分析[J]. 中国药学(英文版), 2020, 29(8): 577-590.

Wen Ma, Shuxiang Song, Jun Li. Surface-assisted laser desorption/ionization mass spectrometry for drug and metabolite analysis[J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(8): 577-590.

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