用电化学方法测定体外聚集过程中β淀粉样多肽含量

doi:10.5246/jcps.2020.03.017

中国药学(英文版) ›› 2020, Vol. 29 ›› Issue (3): 199-205.DOI: 10.5246/jcps.2020.03.017

用电化学方法测定体外聚集过程中β淀粉样多肽含量

张素芳, 李聪, 张莹, 邹俊诚, 崔银株, 凌笑梅^*

北京大学医学部药学院化学生物学系, 北京 100191

收稿日期:2019-06-24 修回日期:2019-08-15 出版日期:2020-03-30 发布日期:2019-12-29
通讯作者: Tel.: +86-10-82801590, E-mail: lingxm@bjmu.edu.cn
基金资助:
The National Youth Foundation of China (Grant No. 81803487), National Natural Science Foundation of China (Grant No. 81673392) and Postdoctoral Science Foundation of China (Grant No. 2018M631285).

Determination of β-amyloid peptides in vitro aggregation process by electrochemistry

Sufang Zhang, Cong Li, Ying Zhang, Juncheng Zou, Yinzhu Cui, Xiaomei Ling^*

Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

Received:2019-06-24 Revised:2019-08-15 Online:2020-03-30 Published:2019-12-29
Contact: Tel.: +86-10-82801590, E-mail: lingxm@bjmu.edu.cn
Supported by:
The National Youth Foundation of China (Grant No. 81803487), National Natural Science Foundation of China (Grant No. 81673392) and Postdoctoral Science Foundation of China (Grant No. 2018M631285).

摘要/Abstract

摘要：

本工作通过合成了富含羧基的铈掺杂氧化锌(CeZnO)纳米粒子作为电极(GCE)修饰材料来固定抗体, 利用乙醇胺(EA)作为封闭剂, 构建了一个用于检测β淀粉样多肽(Aβ)的EA/mAb/CeZnO/GCE阻抗传感器。Aβ的检测线性范围为10–100 pM, 检测限为1.7 pM (S/N = 3)。同根电极和不同电极之间的信号相对标准偏差(RSD)均小于3.0%; 该传感器在4 ºC放置2天, 阻抗值为初始值的101.6%。利用EA/mAb/CeZnO/GCE测量体外孵育后Aβ的剩余量, 结果表明, 在孵育48 h和60 h时Aβ的聚集量基本保持不变, 形成寡聚体和纤维的量达到约90%。本方法具有省时、简单、经济、导电性强、重现性和稳定性好等优势。

关键词: 电化学传感器, 电化学阻抗法, β淀粉样多肽

Abstract:

An impedimetric sensor (EA/mAb/CeZnO/GCE) for detecting β-amyloid peptides (Aβ) was successfully constructed by synthesizing ceria doped zinc oxide (CeZnO) nanoparticles to modify the glassy carbon electrode (GCE) for immobilizing the antibody mAb, and blocking the unbound carboxyl sites with ethanolamine (EA). The sensor exhibited a linear detection range of 10–100 pM and a detection limit of 1.7 pM (S/N = 3), and the relative standard deviations (RSDs) of the inter-electrode and intra-electrodes were less than 3.0%. After it was stored at 4 °C for 2 d, its impedance value was 101.6% of original impedance response. The sensor was used to measure the remaining content of Aβ incubated in vitro. The results showed that after incubation for 48 h and 60 h, the content of aggregated Aβ (oligomers and fibers) almost unchanged, reaching about 90%. This method had the advantages of time-saving, simple procedure, economical, excellent conductivity, good reproducibility and stability.

Key words: Electrochemical sensor, Electrochemical impedance spectroscopy, β-Amyloid peptides

中图分类号:

R917

Supporting:

张素芳, 李聪, 张莹, 邹俊诚, 崔银株, 凌笑梅. 用电化学方法测定体外聚集过程中β淀粉样多肽含量[J]. 中国药学(英文版), 2020, 29(3): 199-205.

Sufang Zhang, Cong Li, Ying Zhang, Juncheng Zou, Yinzhu Cui, Xiaomei Ling. Determination of β-amyloid peptides in vitro aggregation process by electrochemistry[J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(3): 199-205.

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用电化学方法测定体外聚集过程中β淀粉样多肽含量

Determination of β-amyloid peptides in vitro aggregation process by electrochemistry

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