不同溶剂对翅果油树(Elaeagnus mollis)三个部位提取物的抗氧化、酶抑制及抗HepG2细胞增殖活性的研究

doi:10.5246/jcps.2025.05.031

摘要/Abstract

摘要：

为提高翅果油树(Elaeagnus mollis)的潜在应用价值, 本研究采用不同溶剂(水、甲醇、乙醇和正己烷)对翅果油树的不同部位(树枝、树皮和果皮)提取物的化学成分(多酚、黄酮类、生物碱、叶绿素和胡萝卜素)及其抗氧化性能、胆碱酯酶抑制和抗HepG2细胞增殖活性进行了研究。结果表明, 水提取物对ABTS和RP的抗氧化活性最好; 甲醇提取物对DPPH和FRAP的抗氧化活性最好; 甲醇提取物对HepG2细胞增殖的抑制作用最强; 而正己烷提取物对ChE的抑制作用更强。此外, 树皮提取物显示出最高的植物化学成分。树皮和果皮提取物对胆碱酯酶的抑制作用和对HepG2细胞增殖的抑制作用最好, 具有用于医药工业开发为保健品的潜力。

关键词: 翅果油树, 植物化学成分, 抗氧化活性, 胆碱酯酶抑制, 抗HepG2细胞增殖活性

Abstract:

To explore the potential utilization of Elaeagnus mollis, we conducted a comprehensive assessment of its phytochemical composition, antioxidant properties, cholinesterase inhibition, and anti-HepG2 cell proliferation activity across different plant parts (branch wood, branch bark, and pericarp) using various solvents (water, methanol, ethanol, and n-hexane). Our findings revealed that water extracts displayed superior antioxidant activities in ABTS and RP assays, while methanol extracts exhibited better performance in DPPH and FRAP assays. Moreover, methanol extracts demonstrated the highest effectiveness against anti-HepG2 cell proliferation, whereas n-hexane extracts showed greater efficiency in cholinesterase inhibition. Notably, branch bark extracts exhibited the highest levels of phytochemical compounds, with both branch bark and pericarp extracts demonstrating significant effects in cholinesterase inhibition and anti-HepG2 cell proliferation. Correlation analysis indicated that phytochemical compounds were primarily responsible for the observed biological activities. Overall, extracts from the branch bark and pericarp of E. mollis showed promising potential for antioxidant and anticancer activities, suggesting their suitability for applications in the pharmaceutical industry as health-promoting products.

Key words: Elaeagnus mollis, Phytochemical compounds, Antioxidant activity, Cholinesterase inhibitory, Anti-HepG2 cell proliferation activities

Supporting:

钟昊, 李静苗, 李长乐, 刘玉林. 不同溶剂对翅果油树(Elaeagnus mollis)三个部位提取物的抗氧化、酶抑制及抗HepG2细胞增殖活性的研究[J]. 中国药学(英文版), 2025, 34(5): 411-422.

Hao Zhong, Jingmiao Li, Changle Li, Yulin Liu. Evaluation of different solvents for phytochemical compounds, antioxidant activities, cholinesterase inhibition, and anti-HepG2 cell proliferation of three plant parts in Elaeagnus mollis[J]. Journal of Chinese Pharmaceutical Sciences, 2025, 34(5): 411-422.

图/表 6

Figure 1. Plant materials.

Table 1. Total phytochemical compound contents of the tested extracts.

Table 2. Antioxidant capacities of the tested extracts.

Table 3. Cholinesterase inhibition of the tested extracts.

Table 4. Anti-HepG2 cell proliferation activities of the tested extracts.

Figure 2. Spearman correlation coefficient between phytochemical compound contents and antioxidant capacities, cholinesterase inhibition, and anti-HepG2 cell proliferation activities (*P < 0.05; **P < 0.01).

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