不同地域人参药材中人参皂苷的比较研究

doi:10.5246/jcps.2023.12.077

中国药学(英文版) ›› 2023, Vol. 32 ›› Issue (12): 961-970.DOI: 10.5246/jcps.2023.12.077

• 【研究论文】 • 下一篇

不同地域人参药材中人参皂苷的比较研究

孙义政¹, 曲颖², 刘晓艳¹, 徐嵬¹, 张友波¹^,^*()

^1. 北京大学医学部药学院天然药物学系; 天然药物及仿生药物全国重点实验室, 北京 100191
^2. 山东现代学院, 山东济南 250104

收稿日期:2023-07-15 修回日期:2023-08-25 接受日期:2023-11-21 出版日期:2024-01-04 发布日期:2023-12-31
通讯作者: 张友波
基金资助:
National Natural Science Foundation of China (Grant No. 81891011, 81973446).

Comparative study on ginsenosides of ginsengs from different regions

Yizheng Sun¹, Ying Qu², Xiaoyan Liu¹, Wei Xu¹, Youbo Zhang¹^,^*()

¹ State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
² Shandong Xiandai University, Jinan 250104, Shandong, China

Received:2023-07-15 Revised:2023-08-25 Accepted:2023-11-21 Online:2024-01-04 Published:2023-12-31
Contact: Youbo Zhang

摘要/Abstract

摘要：

人参皂苷是人参(Panax ginseng Meyer (P. ginseng))中独特的生物活性成分, 其组成受到地理区域的影响。在中国东北地区, 人参药材生长于多种环境中, 对人参皂苷的形成起到重要影响。本研究针对不同种植区栽培人参的成分差异开展研究。采用超高效液相色谱结合三重四极杆串联飞行时间质谱法对人参皂苷进行表征, 并采用多变量统计分析来确定不同种植区人参药材的成分差异。从不同的人参中共鉴定了359种人参皂苷, 其中包括35种潜在的新成分。在非道地产区及道地产区的人参药材中分别鉴定出283种和312种人参皂苷类成分。该实验结果丰富了人参化合物库, 为人参药材的进一步研究提供了参考依据。

关键词: 人参, 人参皂苷, 生长地域, 多元统计分析, UPLC-MS/MS

Abstract:

Ginsenosides, unique bioactive components found in ginseng (Panax ginseng Meyer), can be influenced by the geographical area in which the ginseng is grown. In northeastern China, ginseng thrives in diverse living environments that play a significant role in shaping the composition of ginsenosides. This study aimed to explore the variations in ginseng constituents cultivated in different growing regions. UPLC coupled with triple quadrupole tandem time-of-flight mass spectrometry was employed to analyze and characterize the ginsenosides, while multivariate statistical analysis was utilized to identify differences among ginseng samples from various regions. A total of 359 ginsenosides, including 35 potential new compounds, were characterized in the different ginseng samples. Among them, 283 and 312 ginsenosides were identified in ginseng from non-traditional and traditional cultivation regions, respectively. These findings contributed to the expansion of the ginseng compound library and provided valuable insights for future research on ginseng.

Key words: Panax ginseng Meyer, Ginsenosides, Growing regions, Multivariate statistical analysis, UPLC-MS/MS

Supporting:

/Jwk3_zgyxen/attached/file/20240104/20240104222856_933.pdf

孙义政, 曲颖, 刘晓艳, 徐嵬, 张友波. 不同地域人参药材中人参皂苷的比较研究[J]. 中国药学(英文版), 2023, 32(12): 961-970.

Yizheng Sun, Ying Qu, Xiaoyan Liu, Wei Xu, Youbo Zhang. Comparative study on ginsenosides of ginsengs from different regions[J]. Journal of Chinese Pharmaceutical Sciences, 2023, 32(12): 961-970.

图/表 5

Figure 1. The BPC (base peak chromatogram) plots of two sample groups and the retention times, molecular ions of 359 ginsenosides.

Figure 2. (A) Five glycosyls and (B) 15 acyls of all ginsenosides of 359 ginsenosides.

Figure 3. Structural analysis of 35 new ginsenosides.

Figure 4. The MS/MS spectra and possible fragmentation process of C7 (A) and C16 (B).

Figure 5. Multivariate statistical analysis. (A) The proportion of different types of ginsenosides in the two growth environment ginseng samples; (B) The Venn diagram of the 359 P. ginseng saponins; (C) Comparison of total saponin content.

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不同地域人参药材中人参皂苷的比较研究

Comparative study on ginsenosides of ginsengs from different regions

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