高效液相色谱法同时测定大鼠血浆中人参皂苷Rg1, Re, Rb1及其在参麦注射液药动学研究中的应用

doi:10.5246/jcps.2015.07.061

中国药学(英文版) ›› 2015, Vol. 24 ›› Issue (7): 475-481.DOI: 10.5246/jcps.2015.07.061

高效液相色谱法同时测定大鼠血浆中人参皂苷Rg1, Re, Rb1及其在参麦注射液药动学研究中的应用

薛清丹¹, 王鹏¹, 康宇红², 李秋红^1*

1. 黑龙江中医药大学药学院临床药学教研室, 黑龙江哈尔滨 150040
2. 黑龙江中医药大学, 教学实验中心, 黑龙江哈尔滨 150040

收稿日期:2015-03-31 修回日期:2015-04-28 出版日期:2015-07-28 发布日期:2015-05-15
通讯作者: Tel.: 86-0451-87266902
基金资助:
Science and Technology Research project of Heilongjiang Province Department of Education (Grant No. 12541740).

Simultaneous determination of ginsenosides Rg1, Re and Rb1 in rat plasma by HPLC and its application to pharmacokinetic study of SHENMAI injection

Qingdan Xue¹, Peng Wang¹, Yuhong Kang², Qiuhong Li^1*

1. Department of Clinical Pharmacy, College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China
2. Teaching and Experiment Center, Heilongjiang University of Chinese Medicine, Harbin 150040, China

Received:2015-03-31 Revised:2015-04-28 Online:2015-07-28 Published:2015-05-15
Contact: Tel.: 86-0451-87266902
Supported by:
Science and Technology Research project of Heilongjiang Province Department of Education (Grant No. 12541740).

摘要/Abstract

摘要：

本文建立了一种灵敏、高效的同时测定大鼠血浆中三种人参皂苷Rg₁, Rb, Re₁的高效液相色谱法。采用C₁₈(150 mm×4.6 mm)色谱柱进行分离; 以乙腈–水为流动相梯度洗脱。通过研究发现标准曲线相关系数高于0.998, 线性范围分别如下: 人参皂苷Rg1为1.0–30.0 μg/mL; 人参皂苷Re为0.5–15.0 μg/mL; 人参皂苷Rb1为0.5–200.0 μg/mL。人参皂苷(Rg₁、Re、Rb₁)三个质控浓度的样品在4 ºC放置24小时, –20 ºC保存30天, 三次冻融循环后均稳定。样品的日内、日间精密度的RSD值均小于6.0%, 平均回收率在96.1%–118.6%之间。人参皂苷Rg₁、Re、Rb₁的最低定量限分别为1.0, 0.5, 0.5 μg/mL。实验证明, 本研究所建立的方法能够成功地应用于大鼠血浆中参麦注射液三种人参皂苷成分(Rg₁、Re、Rb₁)的药动学研究。

关键词: 人参皂苷Rg1, Re, Rb1, 高效液相色谱法, 参麦注射液, 血浆

Abstract:

In the present study, we developed a sensitive and efficient high performance liquid chromatography (HPLC) method for the simultaneous determination of three ginsenosides (Rg₁, Re, Rb₁) in rat plasma. Chromatographic separation was performed on a C₁₈ (150 mm×4.6 mm) column utilizing gradient elution profile and a mobile phase consisting of (A) water and (B) acetonitrile. The calibration curve, with a great correlation coefficient greater than 0.998, was linear over the range of 1.0–30.0 μg/mL for ginsenoside Rg₁, 0.5–15.0 μg/mL for ginsenoside Re, and 0.5–200.0 μg/mL for ginsenoside Rb₁. The intra- and inter-day precisions for three ginsenosides (Rg₁, Re, Rb₁) were all less than 6.0%, and average recovery, examined at three concentration levels, ranged from 96.1% to 118.6%. The samples was stable within 24 h at 4 ºC storage, and 30 d at –20 ºC storage with three freeze-thaw-assay cycles. The low limits of quantification (LOQ) were 1.0, 0.5 and 0.5 μg/mL for Rg₁,Re and Rb₁, respectively. Taken together, the newly developed method was successfully applied to study the pharmacokinetics of ginsenoside Rg₁, Re and Rb₁ in rat plasma after intravenous administration of SHENMAI injection (SMI).

Key words: Ginsenoside Rg1, Re, Rb1, HPLC, SHENMAI injection, Plasma

中图分类号:

R969.1

Supporting:

薛清丹, 王鹏, 康宇红, 李秋红. 高效液相色谱法同时测定大鼠血浆中人参皂苷Rg1, Re, Rb1及其在参麦注射液药动学研究中的应用[J]. 中国药学(英文版), 2015, 24(7): 475-481.

Qingdan Xue, Peng Wang, Yuhong Kang, Qiuhong Li. Simultaneous determination of ginsenosides Rg1, Re and Rb1 in rat plasma by HPLC and its application to pharmacokinetic study of SHENMAI injection[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(7): 475-481.

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高效液相色谱法同时测定大鼠血浆中人参皂苷Rg1, Re, Rb1及其在参麦注射液药动学研究中的应用

Simultaneous determination of ginsenosides Rg1, Re and Rb1 in rat plasma by HPLC and its application to pharmacokinetic study of SHENMAI injection

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