LC-MS/MS测定大鼠体内胡椒酸乙酯的浓度及其降血脂活性研究

doi:10.5246/jcps.2018.12.085

中国药学(英文版) ›› 2018, Vol. 27 ›› Issue (12): 840-846.DOI: 10.5246/jcps.2018.12.085

LC-MS/MS测定大鼠体内胡椒酸乙酯的浓度及其降血脂活性研究

苏日娜¹, 牧丹¹, 萨仁高娃², 麻春杰^3*

1. 内蒙古医科大学药学院, 内蒙古自治区呼和浩特 010110
2. 内蒙古医科大学蒙医药研究院, 内蒙古自治区呼和浩特 010110
3. 内蒙古医科大学中医学院, 内蒙古自治区呼和浩特 010110

收稿日期:2018-07-11 修回日期:2018-08-02 出版日期:2018-12-30 发布日期:2018-11-05
通讯作者: Tel.: +86-13514819729, E-mail: 13514819729@163.com
基金资助:
Clinical Innovative Team of Chinese and Mongolian Medicine (Grant No. YKD2007KJCXTD008).

Anti-hyperlipidaemic assay and LC-MS/MS determination of piperinic ester in rat

Bo Surina¹, Dan Mu¹, Ochir Sarangua², Chunjie Ma^3*

1. College of Pharmacy, Inner Mongolia Medical University, Inner Mongolia, Jinshan Development Zone, Hohhot 010110, China
2. Academy of Mongolian Medicine, Inner Mongolia Medical University, Inner Mongolia, Jinshan Development Zone, Hohhot 010110, China
3. College of traditional Chinese medicine, Inner Mongolia medical University, Inner Mongolia, Jinshan Development Zone, Hohhot 010110, China

Received:2018-07-11 Revised:2018-08-02 Online:2018-12-30 Published:2018-11-05
Contact: Tel.: +86-13514819729, E-mail: 13514819729@163.com
Supported by:
Clinical Innovative Team of Chinese and Mongolian Medicine (Grant No. YKD2007KJCXTD008).

摘要/Abstract

摘要：

本研究旨在建立高脂肪饮食模型,用于评价胡椒酸乙酯的抗高脂血症活性,建立HPLC-MS/MS法测定口服给药后胡椒酸乙酯在大鼠血浆和肝脏中的药代动力学。给大鼠口服施用剂量为50 mg /kg的胡椒酸乙酯化合物。在给药后0.5–10小时通过HPLC检测血浆和肝脏中的胡椒酸乙酯浓度。通过DAS1.0软件分析药代动力学参数。血浆和肝脏胡椒酸乙酯的浓度-时间曲线符合二室模型。胡椒酸乙酯在胃肠道中迅速被吸收,并且可在口服给药后0.5小时在血浆和肝脏中被检测到。

关键词: 荜茇, 胡椒酸乙酯, 降血脂活性, LC-MS/MS分析

Abstract:

In the present study, we aimed to establish high-fat diet model for evaluating anti-hyperlipidaemic activity of GBA and to establish the HPLC-MS method for measuring the pharmacokinetics of GBA in rat blood plasma and liver after oral administration. Rats were orally administrated with GBA at the dose of 50mg/kg body weight. GBA concentrations in blood plasma and liver were detected by HPLC from 0.5–10 h post administrations. The pharmacokinetic parameters were analyzed by the DAS1.0Software. The concentration-time curves of the plasma GBA and liver GBA wereconformed to two-compartment model. GBA was rapidly absorbed in the gastrointestinal tract and could be detected in plasma and liver 0.5 h after oral administration.

Key words: Long pepper, Piperinic ester GBA, Antihyperlipidaemic activity, LC-MS/MS analysis

中图分类号:

R917

Supporting:

苏日娜, 牧丹, 萨仁高娃, 麻春杰. LC-MS/MS测定大鼠体内胡椒酸乙酯的浓度及其降血脂活性研究[J]. 中国药学(英文版), 2018, 27(12): 840-846.

Bo Surina, Dan Mu, Ochir Sarangua, Chunjie Ma. Anti-hyperlipidaemic assay and LC-MS/MS determination of piperinic ester in rat[J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(12): 840-846.

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LC-MS/MS测定大鼠体内胡椒酸乙酯的浓度及其降血脂活性研究

Anti-hyperlipidaemic assay and LC-MS/MS determination of piperinic ester in rat

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