左旋-5­羟基-雌马酚对秀丽隐杆线虫寿命及抗性的影响

doi:10.5246/jcps.2014.06.051

中国药学(英文版) ›› 2014, Vol. 23 ›› Issue (6): 378-384.DOI: 10.5246/jcps.2014.06.051

左旋-5羟基-雌马酚对秀丽隐杆线虫寿命及抗性的影响

张聪慧¹, 王秀伶^1*, 梁晓琳^1,2, 张红蕾¹, 郝庆红¹

1. 河北农业大学生命科学学院, 河北保定 071000
2. 华北制药集团, 河北石家庄 050000

收稿日期:2014-03-24 修回日期:2014-03-31 出版日期:2014-06-28 发布日期:2014-04-20
通讯作者: Tel.: 86-312-7528257, Fax: 86-312-7528265
基金资助:
National Natural Science Foundation of China (Grant No. 31170058) and the Service Center for Experts and Scholars of Hebei Province (Grant No. CPRC027).

Effects of (–)-5-hydroxy-equol on the lifespan and stress resistance of Caenorhabditis elegans

Conghui Zhang¹, Xiuling Wang^1*, Xiaolin Liang^1,2, Honglei Zhang¹, Qinghong Hao¹

1. College of Life Sciences, Agricultural University of Hebei, Baoding 071000, China
2. North China Pharmaceutical Group Corporation, Shijiazhuang 050000, China

Received:2014-03-24 Revised:2014-03-31 Online:2014-06-28 Published:2014-04-20
Contact: Tel.: 86-312-7528257, Fax: 86-312-7528265
Supported by:
National Natural Science Foundation of China (Grant No. 31170058) and the Service Center for Experts and Scholars of Hebei Province (Grant No. CPRC027).

摘要/Abstract

摘要：

以秀丽隐杆线虫模式生物为研究对象, 研究大豆异黄酮染料木素微生物代谢产物左旋-5-羟基-雌马酚对线虫寿命、生殖能力以及抗热应激和抗氧化应激能力的影响。研究结果表明, 浓度为0.1 mmol/L和0.2 mmol/L的左旋-5-羟基-雌马酚不仅能够显著延长线虫寿命, 同时还能显著增强线虫耐热和抗氧化应激能力, 而在相同浓度下线虫生殖能力则无明显不良影响。通过进一步对比分析转录物水平以及喂食相应线虫突变体daf-16 (mu86) 和daf-2 (e1370), 确定左旋-5-羟基-雌马酚延长线虫寿命与DAF-2/DAF-16 Insulin/IGF-1信号转导通路相关。有关左旋-5-羟基-雌马酚显著延长线虫寿命和增强线虫抗性为国内外首次报道。

关键词: 左旋-5羟基雌马酚, 秀丽隐杆线虫, 寿命, 热应激, 氧化应激, 生殖能力, Insulin/IGF-1信号通路

Abstract:

Caenorhabditis elegans (C. elegans) was used as an animal model to study the effect of (–)-5-hydroxy-equol, a microbial metabolite of isoflavone genistein, on the lifespan, fecundity and resistance against thermal and oxidative stress. The results showed that (–)-5-hydroxy-equol not only significantly increased the lifespan of C. elegans but alsosignificantly enhanced the resistance against thermal and oxidative stress at the concentrations of 0.1 mmol/L and 0.2 mmol/L. However, the fecundity ofC.elegans was not obviously influenced after being exposed to the same concentrations of (–)-5-hydroxy-equol. Furtherstudieson comparative transcriptome analyses and the lifespan of daf-16 (mu86) mutant and daf-2 (e1370) mutantindicated that(–)-5-hydroxy-equol prolonged the lifespan of C.elegansthrough DAF-2/DAF-16 Insulin/IGF-1 signaling pathway.This is the first report that (–)-5-hydroxy-equol was able to increase the lifespan and improve the thermal and oxidative stress tolerance of C. elegans.

Key words: (–)-5-Hydroxy-equol, Caenorhabditis elegans, Lifespan, Thermal stress, Oxidative stress, Fecundity, Insulin/IGF-1 signaling pathwa

中图分类号:

R962.1

Supporting: National Natural Science Foundation of China (Grant No. 31170058) and the Service Center for Experts and Scholars of Hebei Province (Grant No. CPRC027).

张聪慧, 王秀伶, 梁晓琳, 张红蕾, 郝庆红. 左旋-5羟基-雌马酚对秀丽隐杆线虫寿命及抗性的影响[J]. 中国药学(英文版), 2014, 23(6): 378-384.

Conghui Zhang, Xiuling Wang, Xiaolin Liang, Honglei Zhang, Qinghong Hao. Effects of (–)-5-hydroxy-equol on the lifespan and stress resistance of Caenorhabditis elegans[J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(6): 378-384.

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左旋-5羟基-雌马酚对秀丽隐杆线虫寿命及抗性的影响

Effects of (–)-5-hydroxy-equol on the lifespan and stress resistance of Caenorhabditis elegans

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左旋-5­羟基-雌马酚对秀丽隐杆线虫寿命及抗性的影响

Effects of (–)-5-hydroxy-equol on the lifespan and stress resistance of Caenorhabditis elegans

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左旋-5羟基-雌马酚对秀丽隐杆线虫寿命及抗性的影响