Eu3+掺杂的当归多糖: 结构, 形貌特征及抗菌活性评价

doi:10.5246/jcps.2026.02.009

中国药学(英文版) ›› 2026, Vol. 35 ›› Issue (2): 131-143.DOI: 10.5246/jcps.2026.02.009

Eu³⁺掺杂的当归多糖: 结构, 形貌特征及抗菌活性评价

侯继霞¹^,², 张腾予¹^,², 赵洁盈¹, 冯若飞²^,³^,^*(), 谈春霞¹^,²^,^*()

^1. 甘肃中医药大学, 甘肃兰州 730000
^2. 甘肃省中医药研究中心, 甘肃兰州 730000
^3. 西北民族大学, 甘肃兰州 730000

收稿日期:2025-10-13 修回日期:2025-11-21 接受日期:2026-01-06 出版日期:2026-03-05 发布日期:2026-03-05
通讯作者: 冯若飞, 谈春霞

Structural, morphological, and antibacterial characterization of Eu³⁺ doped Angelica sinensis polysaccharides

Jixia Hou¹^,², Tengyu Zhang¹^,², Jieying Zhao¹, Ruofei Feng²^,³^,^*(), Chunxia Tan¹^,²^,^*()

^1. Gansu University of Chinese Medicine, 730000 Lanzhou, Gansu, China
^2. Research Center of Traditional Chinese Medicine, 730000 Lanzhou, Gansu, China
^3. Northwest Minzu University, 730000 Lanzhou, Gansu, China

Received:2025-10-13 Revised:2025-11-21 Accepted:2026-01-06 Online:2026-03-05 Published:2026-03-05
Contact: Ruofei Feng, Chunxia Tan
Supported by:
2022 Natural Science Foundation of Gansu Province (CN) (Grant No. 22JR11RA119); Biomedical Research Center of Northwest Minzu University (Grant No. BRC-KF202303); Research Center of Traditional Chinese Medicine, Gansu Province (Grant No. zyzx-2023-22).

摘要/Abstract

摘要：

本研究对制备的掺杂发光元素Eu的当归多糖复合物(ASP/Eu)进行了热稳定性、聚集形态、构象、发光性能和抑菌活性的研究。Eu元素的存在增加了多糖的热稳定性, 但改变了其构象。刚果红试验结果表明, ASP/Eu复合物没有三螺旋结构, 可能形成了具有荧光性质的更紧密的糖链聚集体。在固态和水溶液中都可以观察到稀土掺杂多糖复合物的呈现的弱荧光。扫描电镜和透射电镜的测试结果也证实了这一猜测。ASP/Eu对大肠杆菌和金黄色葡萄球菌的最低抑菌浓度(MIC)均为800 μg/mL, 大肠杆菌和金黄色葡萄球菌存活率分别为5.38% ± 0.53%和4.92% ± 1.49%。复合物的抗菌实验证明: 和当归多糖相比, 掺杂Eu的多糖复合物具有更好的抑菌效果。

关键词: 发光多糖, 形貌, 结构, 抑菌活性

Abstract:

In the present study, a luminescent hybrid material (ASP/Eu) was synthesized by incorporating Eu³⁺ into Angelica sinensis polysaccharides. Comprehensive characterization was conducted to evaluate its thermal stability, aggregate morphology, conformational features, photophysical behavior, and antibacterial activity. Incorporation of Eu enhanced the thermal stability of the polysaccharide matrix while inducing conformational alterations. Congo Red assays indicated that ASP/Eu lacked a triple-helix architecture and instead assembled into more compact sugar-chain aggregates exhibiting fluorescence. Weak luminescence from the Eu-doped polysaccharide was detectable both in the solid state and in aqueous solution, an observation further corroborated by SEM and TEM analyses. The minimum inhibitory concentration (MIC) of ASP/Eu was 800 μg/mL, reducing the survival rate of E. coli to 5.38% ± 0.53% and S. aureus to 4.92% ± 1.49%. These antibacterial assays demonstrated that Eu incorporation significantly improved the bioactivity of the hybrid. Collectively, these findings provided a valuable foundation for advancing the biological exploration and potential applications of ASP/Eu hybrids.

Key words: Luminescent polysaccharide, Morphology, Structure, Antibacterial activity

Supporting:

侯继霞, 张腾予, 赵洁盈, 冯若飞, 谈春霞. Eu³⁺掺杂的当归多糖: 结构, 形貌特征及抗菌活性评价[J]. 中国药学(英文版), 2026, 35(2): 131-143.

Jixia Hou, Tengyu Zhang, Jieying Zhao, Ruofei Feng, Chunxia Tan. Structural, morphological, and antibacterial characterization of Eu³⁺ doped Angelica sinensis polysaccharides[J]. Journal of Chinese Pharmaceutical Sciences, 2026, 35(2): 131-143.

图/表 10

Figure 1. (a) IR full spectrum of ASP/Eu and ASP; (b) locally amplified IR spectra.

Figure 2. XPS spectra (a) Full spectrum of ASP/Eu; (b) O in ASP/Eu; (c) Eu in ASP/Eu.

Figure 3. Fluorescence spectra (a) and images of (b) aggregates in aqueous solution and (c) solid powder.

Figure 4. TG-DTG curves of ASP/Eu.

Figure 5. Congo red results of ASP and ASP/Eu.

Figure 6. XRD curve of samples.

Figure 7. SEM photos of (a) ASP and (b) ASP/Eu.

Figure 8. SEM-EDS analysis of ASP/Eu (a) secondary electron imaging; (b) backscattered electron imaging; (c) EDS spectra and EDS tabulated results.

Figure 9. TEM images of (a) ASP and (b) magnified ASP; (c) ASP/Eu and (d) edge of magnified.

Figure 10. Survival rate of ASP and ASP/Eu on (a) E. coli and (b) S. aureus. ***P < 0.001, **P < 0.01, *P < 0.05, ns: no significant difference.

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Eu³⁺掺杂的当归多糖: 结构, 形貌特征及抗菌活性评价

Structural, morphological, and antibacterial characterization of Eu³⁺ doped Angelica sinensis polysaccharides

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