基于网络药理学-分子对接探析灭幽汤

doi:10.5246/jcps.2024.03.021

中国药学(英文版) ›› 2024, Vol. 33 ›› Issue (3): 258-271.DOI: 10.5246/jcps.2024.03.021

• 【研究论文】 • 上一篇

基于网络药理学-分子对接探析灭幽汤"异病同治"慢性萎缩性胃炎和胃溃疡的作用机制

王思宇¹, 周淑伟², 喻斌¹^,^*()

^1. 湖南中医药大学第一附属医院, 湖南长沙 410007
^2. 东南大学附属中大医院, 江苏南京 210009

收稿日期:2023-09-24 修回日期:2023-10-27 接受日期:2023-11-15 出版日期:2024-03-31 发布日期:2024-03-31
通讯作者: 喻斌

Exploring the mechanism of Mie-you decoction "homotherapy for heteropathy" to chronic atrophic gastritis and gastric ulcer using network pharmacology-molecular docking

Siyu Wang¹, Shuwei Zhou², Bin Yu¹^,^*()

¹ Department of Gastroenterology, The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan, China
² Department of Radiology, Jiangsu Key Laboratory of Molecular and Functional Imaging, Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu, China

Received:2023-09-24 Revised:2023-10-27 Accepted:2023-11-15 Online:2024-03-31 Published:2024-03-31
Contact: Bin Yu
Supported by:
Hunan Provincial Department of Education Scientific Research Project (Grant No. 20A371); Key Project of Hunan Administration of Traditional Chinese Medicine (Grant No. C2022016); Research Program Project of Hunan University of Traditional Chinese Medicine (Grant No. zyydx201727); Clinical Technology Innovation Project of Hunan Provincial Department of Science and Technology (Grant No. 2021SK51403).

摘要/Abstract

摘要：

本研究旨在运用网络药理学分析方法与分子对接技术研究灭幽汤异病同治慢性萎缩性胃炎和胃溃疡的作用机制。运用TCMSP和BATMAN-TCM数据库筛选出灭幽汤的有效成分, Genecards、OMIM、Disgenet、Drugbank数据库筛选出慢性萎缩性胃炎和胃溃疡的相关靶点; 采用Cytoscape 3.9.1软件绘制"方剂-药物-活性成分-核心靶点-疾病"的网络拓扑图; 采用STRING数据库构建蛋白互作网络; 运用David数据库对核心靶点进行GO富集分析及KEGG通路分析; 采用AutoDock4软件进行分子对接预测药物与疾病靶点的结合性, 通过PyMOL 2.2软件实现对接结果可视化。结果得到灭幽汤有效成分73个, CAG相关靶点761个, GU相关靶点640个, 通过Venny图取交集后得到54个靶点, Cytoscape筛选后得核心靶点37个。分子对接结果显示, TP53、IL6等5种蛋白与木犀草素(luteolin)、金合欢素(acacetin)、柚皮素(naringenin)、黄芩苷(baicalein)、汉黄芩素(wogonin)有较好的结合活性; KEGG富集分析主要得到癌症通路、脂质与动脉粥样硬化、PI3K-Akt信号通路、MAPK信号通路、IL-17信号通路、AGE-RAGE信号通路等。研究表明, 灭幽汤异病同治慢性萎缩性胃炎和胃溃疡具有多成分、多靶点及多通路的特性, 其主要成分与活性分子靶点结合, 通过调控癌症、脂质与动脉粥样硬化等信号通路实现"同治"CAG和GU的目的。为传统中医药的"异病同治"理论提供了现代医学证据, 并且为今后的新药研发与实验设计指明了方向。

关键词: 网络药理学, 分子对接, 慢性萎缩性胃炎, 胃溃疡, 异病同治, 灭幽汤

Abstract:

In this study, we employed a network pharmacology-molecular docking-based strategy to investigate the mechanism of Mie-you decoction (MYT) in treating chronic atrophic gastritis (CAG) and gastric ulcer (GU) based on the theory of "homotherapy for heteropathy". First, we searched for the bioactive ingredients of MYT using the TCMSP and BATMAN-TCM databases. Then, we identified the corresponding protein targets for CAG and GU by searching the GeneCards, OMIM, DisGeNET, and DrugBank databases. Using Cytoscape 3.9.1, we constructed a "drug-ingredients-target-pathway-disease" network. The protein-protein interaction (PPI) network was generated using the STRING network platform. To further analyze the core targets, we performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses using the DAVID online tool. AutoDock4 was utilized to assess the binding affinity between ingredients and core targets, and the molecule-protein interactions were visualized using PyMOL 2.2. Our findings revealed a total of 73 bioactive ingredients with 101 potential targets for MYT. Among them, 54 potential targets were identified for both CAG and GU. Ultimately, 37 proteins were determined to be the core targets of MYT in combating CAG and GU. Molecular docking analysis demonstrated high affinity between five proteins, including tumor suppressor protein p53 (TP53) and interleukin-6 (IL-6), and bioactive ingredients, such as luteolin, acacetin, naringenin, baicalein, and wogonin. KEGG pathway enrichment analysis revealed 152 signaling pathways that might play crucial roles in the effectiveness of MYT against CAG and GU. The characteristics of MYT, including its multi-component, multi-target, and multi-pathway effects, aligned with the concept of "homotherapy for heteropathy" in both CAG and GU. By effectively modulating various signaling pathways, including those related to cancer, lipid metabolism, atherosclerosis, and others, MYT achieved its therapeutic objectives. These findings provided modern medical evidence supporting traditional Chinese medicine theory and offered directions for future drug development and experimental design.

Key words: Network pharmacology, Molecular docking, Chronic atrophic gastritis, Gastric ulcer, Homotherapy for heteropathy, Mie-you decoction

Supporting:

王思宇, 周淑伟, 喻斌. 基于网络药理学-分子对接探析灭幽汤"异病同治"慢性萎缩性胃炎和胃溃疡的作用机制[J]. 中国药学(英文版), 2024, 33(3): 258-271.

Siyu Wang, Shuwei Zhou, Bin Yu. Exploring the mechanism of Mie-you decoction "homotherapy for heteropathy" to chronic atrophic gastritis and gastric ulcer using network pharmacology-molecular docking[J]. Journal of Chinese Pharmaceutical Sciences, 2024, 33(3): 258-271.

图/表 9

Table 1. Top 10 potential bioactive ingredients of MYT.

Figure 1. Venny diagram of the treatment of CAG and GU targets with Mie-you decoction. Note: blue represents the number of targets for CAG, yellow represents the number of targets for GU, green represents the number of targets for MYT, and the middle part represents the intersection targets of the three.

Figure 2. The "drug-disease" core target protein interaction network of MYT in the treatment of CAG and GU. Note: Nodes represent proteins (The colors from yellow to blue and the size from small to large represent the degree of each protein). Edge represents protein-protein association (The lines from thin to thick and the color from light to dark reflect the betweenness value between proteins).

Table 2. 37 core targets information of PPI network.

Figure 3. Drug-ingredients-target-disease network. Note: the red hexagon nodes represent CAG and GU. The blue diamond nodes represent the target proteins. The orange oval nodes represent the bioactive ingredients of herbs. The light green triangle nodes represent herbs of MYT, including Scutellariae Radix (HQ), Citrus Reticulata (CP), Citri Reticulatae Pericarpium Viride (QP), Bletilla Striata (BJ), PanaxNotoginseng (SQ) and Taraxaci Herba (PGY). The green rectangular node represents MYT. Edges represent the interactions between goals and targets.

Figure 4. GO enrichment analysis of CAG and GU. Note: green represents BP, red represents CC, and blue represents MF.

Figure 5. Top 25 KEGG enrichment analyses of CAG and GU. Note: A is the bubble diagram, and B is the network topology diagram.

Table 3. Molecular docking results of the bioactive ingredients of MYT and the core targets of CAG and GU.

Figure 6. Partial molecular docking results of main bioactive ingredients of Mie-you decoction. Note: (A) AKT1-baicalein; (B) IL6-luteolin; (C) PTGS2-acacetin; (D) TP53-naringenin; (E) VEGFA-wogonin.

参考文献 47

[1]	Pimentel-Nunes, P.; Libânio, D.; Marcos-Pinto, R.; Areia, M.; Leja, M.; Esposito, G.; Garrido, M.; Kikuste, I.; Megraud, F.; Matysiak-Budnik, T.; Annibale, B.; Dumonceau, J.M.; Barros, R.; Fléjou, J.F.; Carneiro, F.; van Hooft, J.E.; Kuipers, E.J.; Dinis-Ribeiro, M. Management of epithelial precancerous conditions and lesions in the stomach (MAPS II): European Society of Gastrointestinal Endoscopy (ESGE), European Helicobacter and Microbiota Study Group (EHMSG), European Society of Pathology (ESP), and Sociedade Portuguesa de Endoscopia Digestiva (SPED) guideline update 2019. Endoscopy. 2019, 51, 365–388.
[2]	Sant, D.W.; Camarena, V.; Mustafi, S.; Li, Y.W.; Wilkes, Z.; Van Booven, D.; Wen, R.; Wang, G.F. Ascorbate suppresses VEGF expression in retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 2018, 59, 3608.
[3]	Misiewicz, J.J. The Sydney System: a new classification of gastritis. Introduction. J. Gastroenterol. Hepatol. 1991, 6, 207–208.
[4]	Du, Y.Q.; Bai, Y.; Xie, P.; Fang, J.Y.; Wang, X.Z.; Hou, X.H.; Tian, D.A.; Wang, C.D.; Liu, Y.D.; Sha, W.H.; Wang, B.M.; Li, Y.Q.; Zhang, G.L.; Li, Y.; Shi, R.H.; Xu, J.M.; Li, Y.M.; Huang, M.H.; Han, S.X.; Liu, J.; Ren, X.; Xie, P.Y.; Wang, Z.L.; Cui, L.H.; Sheng, J.Q.; Luo, H.S.; Wang, Z.H.; Zhao, X.Y.; Dai, N.; Nie, Y.Q.; Zou, Y.Y.; Xia, B.; Fan, Z.N.; Chen, Z.T.; Lin, S.R.; Li, Z.S.; Group, C.C.G.R. Chronic gastritis in China: a national multi-center survey. BMC Gastroenterol. 2014, 14, 21.
[5]	da Silva, D.M.; Martins, J.L.R.; de Oliveira, D.R.; Florentino, I.F.; da Silva, D.P.B.; dos Santos, F.C.A.; Costa, E.A. Effect of allantoin on experimentally induced gastric ulcers: pathways of gastroprotection. Eur. J. Pharmacol. 2018, 821, 68–78.
[6]	Tabiri, S.; Akanbong, P.; Abubakari, B.B. Assessment of the environmental risk factors for a gastric ulcer in northern Ghana. Pan Afr. Med. J. 2016, 25, 160.
[7]	Ford, A.C.; Gurusamy, K.S.; Delaney, B.; Forman, D.; Moayyedi, P. Eradication therapy for peptic ulcer disease in Helicobacter pylori-positive people. Cochrane Database Syst. Rev. 2016, 4, CD003840.
[8]	Dang, N.Q.H.; Ha, T.M.T.; Nguyen, S.T.; Le, N.D.K.; Nguyen, T.M.T.; Nguyen, T.H.; Pham, T.T.H.; Tran, V.H. High rates of clarithromycin and levofloxacin resistance of Helicobacter pylori in patients with chronic gastritis in the south east area of Vietnam. J. Glob. Antimicrob. Resist. 2020, 22, 620–624.
[9]	Liu, W.Z.; Xie, Y.; Lu, H.; Cheng, H.; Zeng, Z.R.; Zhou, L.Y.; Chen, Y.; Wang, J.B.; Du, Y.Q.; Lu, N.H.; Chinese Study Group on Helicobacter pylori and Peptic Ulcer Chinese Society of Gastroenterology. Fifth Chinese National Consensus Report on the management of Helicobacter pylori infection. Helicobacter. 2018, 23, e12475.
[10]	Yang, Y.; Zhang, S.; Liu, M.; Liu, D.; Wang, Y.; Du, G. Network pharmacological analysis of Xiao-Xu-Ming Decoction against ischemic stroke and verification of its mechanism of anti-inflammation and neurovascular protection in vivo. J. Chin. Pharm. Sci. 2022, 31, 343.
[11]	Wang, P.; Tang, S.H.; Su, J.; Zhang, J.Q.; Cui, R.Y.; Xu, H.Y.; Yang, H.J. Modern research progress of traditional Chinese medicine based on integrative pharmacology. China J. Chin. Mater. Med. 2018, 43, 1297.
[12]	Wang, X.; Yu, H.; Bai, C.; Xu, J.N.; Zhen, J.H.; Gu, X.H. Pharmacological mechanism of Shenghua Decoction in treatment of abdominal pain based on network pharmacology. China J. Chin. Mater. Med. 2019, 44, 2124–2130.
[13]	Liu, S.; Zhu, J.J.; Li, J.C. The interpretation of human body in traditional Chinese medicine and its influence on the characteristics of TCM theory. Anat. Rec. 2021, 304, 2559–2565.
[14]	Li, W.W.; Guo, H.; Wang, X.M. Relationship between endogenous hydrogen sulfide and blood stasis syndrome based on the Qi-blood theory of Chinese medicine. Chin. J. Integr. Med. 2013, 19, 701–705.
[15]	Lahner, E.; Carabotti, M.; Annibale, B. Treatment of Helicobacter pylori infection in atrophic gastritis. World J. Gastroenterol. 2018, 24, 2373–2380.
[16]	Yu, C.J.; Qiu, J.Y.; Xiong, M.C.; Ou, C.; Zeng, M.Y.; Song, H.P. Trends in Helicobacter pylori-related gastric ulcer research from 2012 to 2022: a bibliometric and visual analysis. Front. Med. 2022, 9, 1027534.
[17]	Tiwari, A.; Rai, R.; Dahal, P.; Regmi, S. Prevalence of helicobacter pylori in endoscopic gastric biopsies of chronic gastritis patients at A tertiary care centre. J. Nepal Med. Assoc. 2020, 58, 564.
[18]	Ding, H.M.; Xue, J.D. Pathogenesis and Development of HP-related Peptic Ulcer Potential Correlation with the Theory of Toxic Heat in Chinese Medicine. J. Liaoning Univ. TCM. 2022, 1673, 108.
[19]	Zheng, H.; Wang, C.; Wang, W.; Cai, L.; Fu, H.; Zhou, X. Systematic Study on "Toxin-heat" as an Innovative Etiology of Gastric Ulcer in Active State. World Chin. Med. 2014, 9, 557.
[20]	Bin, Y.U.; Luo, Y.; Wang, X.J.; Shu, H.U.; Zhong, D.U.; Yin, J.; Xia, R. Effect of Mieyou decoction on TLR4/NF-κB65 signal pathway in the H. Pylori-associated gastritis mice of spleen-stomach damp-heat syndrome. Chin. J. Integr. Tradit. West. Med. Dig. 2014, 22, 369.
[21]	Zeng, R.; Yu，B.; Xu，Y.; Zhou, Y.F.; Mo, C. Effect of Mieyou Decoction on PTEN-PI3K-Akt-FoxO apoptotic signal pathway in the H.Pylori-associated gastritis mice with spleen-stomach damp-heat syndrome. Mod. J. Integr. Tradit. Chin. West. Med. 2020, 26, 2869.
[22]	Franza, L.; Carusi, V.; Nucera, E.; Pandolfi, F. Luteolin, inflammation and cancer: special emphasis on gut microbiota. BioFactors. 2021, 47, 181–189.
[23]	Singh, S.; Gupta, P.; Meena, A.; Luqman, S. Acacetin, a flavone with diverse therapeutic potential in cancer, inflammation, infections and other metabolic disorders. Food Chem. Toxicol. 2020, 145, 111708.
[24]	Wang, Z.L.; Wang, S.; Kuang, Y.; Hu, Z.M.; Qiao, X.; Ye, M. A comprehensive review on phytochemistry, pharmacology, and flavonoid biosynthesis of Scutellaria baicalensis. Pharm. Biol. 2018, 56, 465–484.
[25]	Min, L.W. New therapeutic aspects of flavones: the anticancer properties of Scutellaria and its main active constituents Wogonin, Baicalein and Baicalin. Cancer Treat. Rev. 2009, 35, 57–68.
[26]	Zaidun, N.H.; Thent, Z.C.; Latiff, A.A. Combating oxidative stress disorders with citrus flavonoid: Naringenin. Life Sci. 2018, 208, 111–122.
[27]	Sowndhararajan, K.; Deepa, P.; Kim, M.; Park, S.J.; Kim, S. Baicalein as a potent neuroprotective agent: a review. Biomed. Pharmacother. 2017, 95, 1021–1032.
[28]	Boutelle, A.M.; Attardi, L.D. p53 and tumor suppression: it takes a network. Trends Cell Biol. 2021, 31, 298–310.
[29]	Shiao, Y.H.; Rugge, M.; Correa, P.; Lehmann, H.P.; Scheer, W.D. p53 alteration in gastric precancerous lesions. Am. J. Pathol. 1994, 144, 511–7.
[30]	Fenoglio-Preiser, C.M.; Wang, J.; Stemmermann, G.N.; Noffsinger, A. TP53 and gastric carcinoma: a review. Hum. Mutat. 2003, 21, 258–270.
[31]	Okimoto K Murakami T Fujioka T. Analysis of p53 gene mutations in Helicobacter pylori-associated gastritis mucosa in endoscopic biopsy specimens. Scand. J. Gastroenterol. 1999, 34, 474–477.
[32]	Guerau-de-Arellano, M.; Piedra-Quintero, Z.L.; Tsichlis, P.N. Akt isoforms in the immune system. Front. Immunol. 2022, 13, 990874.
[33]	Song, G.; Ouyang, G.L.; Bao, S.D. The activation of Akt/PKB signaling pathway and cell survival. J. Cell Mol. Med. 2005, 9, 59–71.
[34]	Hirano, T. IL-6 in inflammation, autoimmunity and cancer. Int. Immunol. 2021, 33, 127–148.
[35]	Ferrara, N.; Adamis, A.P. Ten years of anti-vascular endothelial growth factor therapy. Nat. Rev. Drug Discov. 2016, 15, 385–403.
[36]	Huang, J.Q.; Zagai, U.; Hallmans, G.; Nyrén, O.; Engstrand, L.; Stolzenberg-Solomon, R.; Duell, E.J.; Overvad, K.; Katzke, V.A.; Kaaks, R.; Jenab, M.; Park, J.Y.; Murillo, R.; Trichopoulou, A.; Lagiou, P.; Bamia, C.; Bradbury, K.E.; Riboli, E.; Aune, D.; Tsilidis, K.K.; Capellá, G.; Agudo, A.; Krogh, V.; Palli, D.; Panico, S.; Weiderpass, E.; Tjønneland, A.; Olsen, A.; Martínez, B.; Redondo-Sanchez, D.; Chirlaque, M.D.; Peeters, P.H.; Regnér, S.; Lindkvist, B.; Naccarati, A.; Ardanaz, E.; Larrañaga, N.; Boutron-Ruault, M.C.; Rebours, V.; Barré, A.; As Bueno-de-Mesquita, H.B.; Ye, W.M. Helicobacter pylori infection, chronic corpus atrophic gastritis and pancreatic cancer risk in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort: a nested case-control study. Int. J. Cancer. 2017, 140, 1727–1735.
[37]	Wallace, J.L.; Dicay, M.; McKnight, W.; Dudar, G.K. Platelets accelerate gastric ulcer healing through presentation of vascular endothelial growth factor. Br. J. Pharmacol. 2006, 148, 274–278.
[38]	Guo, L.T.; Wang, S.Q.; Su, J.; Xu, L.X.; Ji, Z.Y.; Zhang, R.Y.; Zhao, Q.W.; Ma, Z.Q.; Deng, X.Y.; Ma, S.P. Baicalin ameliorates neuroinflammation-induced depressive-like behavior through inhibition of toll-like receptor 4 expression via the PI3K/AKT/FoxO1 pathway. J. Neuroinflammation. 2019, 16, 1–21.
[39]	Fu, Y.J.; Xu, B.; Huang, S.W.; Luo, X.; Deng, X.L.; Luo, S.; Liu, C.; Wang, Q.; Chen, J.Y.; Zhou, L. Baicalin prevents LPS-induced activation of TLR4/NF-κB p65 pathway and inflammation in mice via inhibiting the expression of CD14. Acta Pharmacol. Sin. 2021, 42, 88–96.
[40]	Meng, F.J.; Yang, S.G.; Wang, X.; Chen, T.T.; Wang, X.L.; Tang, X.Y.; Zhang, R.J.; Shen, L. Reclamation of Chinese herb residues using probiotics and evaluation of their beneficial effect on pathogen infection. J. Infect. Public Health. 2017, 10, 749–754.
[41]	Yu, X.D.; Zheng, R.B.; Xie, J.H.; Su, J.Y.; Huang, X.Q.; Wang, Y.H.; Zheng, Y.F.; Mo, Z.Z.; Wu, X.L.; Wu, D.W.; Liang, Y.E.; Zeng, H.F.; Su, Z.R.; Huang, P. Biological evaluation and molecular docking of baicalin and scutellarin as Helicobacter pylori urease inhibitors. J. Ethnopharmacol. 2015, 162, 69–78.
[42]	Faria, T.C.; Nascimento, C.C.H.C.; De Vasconcelos, S.D.D.; Stephens, P.R.S. Literature review on the biological effects of taraxacum officinale plant in therapy. Asian J. Pharm. Res. Dev. 2019, 7, 94–99.
[43]	Orozco, M.F.; Vázquez-Hernández, A.; Fenton-Navarro, B. Active compounds of medicinal plants, mechanism for antioxidant and beneficial effects. Revista Internacional De Botánica Exper. Inter. J. Exper. Botany. 2019, 88, 1–10.
[44]	Mao, Y. Analysis of anti-inflammatory, immune and anti-aging pharmacological studies of flavonoids. J. China Prescript. Drug. 2019, 17, 39.
[45]	Almajano, M.P.; Carbó, R.; Delgado, M.E.; Gordon, M.H. Effect of pH on the antimicrobial activity and oxidative stability of oil-in-water emulsions containing caffeic acid. J. Food Sci. 2007, 72, C258–C263.
[46]	Park, C.M.; Jin, K.S.; Lee, Y.W.; Song, Y.S. Luteolin and chicoric acid synergistically inhibited inflammatory responses via inactivation of PI3K-Akt pathway and impairment of NF-κB translocation in LPS stimulated RAW 264.7 cells. Eur. J. Pharmacol. 2011, 660, 454–459.
[47]	Yan, Y.; Zhang, Y.; Wu, Y.; Yang, R. Optimization of Extraction Process of Total Flavonoids from Taraxacum and its Antibacterial Activity. Guangxi Forestry Sci. 2019, 48, 223.

基于网络药理学-分子对接探析灭幽汤"异病同治"慢性萎缩性胃炎和胃溃疡的作用机制

Exploring the mechanism of Mie-you decoction "homotherapy for heteropathy" to chronic atrophic gastritis and gastric ulcer using network pharmacology-molecular docking

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