采用UFLC-IT-TOF/MS鉴定大鼠口服黄芩提取物后胆汁、血浆和尿液中的主要成分

doi:10.5246/jcps.2013.04.046

采用UFLC-IT-TOF/MS鉴定大鼠口服黄芩提取物后胆汁、血浆和尿液中的主要成分

陈正为, 佟玲, 李淑明, 李东翔, 刘小琳, 丁黎^*, 朱永宏, 周水平, 孙鹤

1. 中国药科大学药物分析研究室, 江苏南京 210009
2. 天士力集团研究院, 天津 300402
3. 中国药科大学药物代谢研究中心, 江苏南京 210009

收稿日期:2012-11-21 修回日期:2012-12-18 出版日期:2013-07-10 发布日期:2013-07-10
通讯作者: 丁黎*

Identification of major parent compounds and metabolites in bile, plasma and urine of rats after oral administration of Radix Scutellariae extract by UFLC-IT-TOF/MS

Zhengwei Chen, Ling Tong, Shuming Li, Dongxiang Li, Xiaolin Liu, Li Ding^*, Yonghong Zhu, Shuiping Zhou, He Sun

1. Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China
2.Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., Tianjin 300402,China
3.Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China

Received:2012-11-21 Revised:2012-12-18 Online:2013-07-10 Published:2013-07-10
Contact: Li Ding*

摘要/Abstract

摘要：

黄酮类化合物是中国传统中药黄芩的主要活性成分。大鼠口服黄芩提取物后, 采用超快速液相色谱-离子阱飞行时间质谱(UFLC-IT-TOF/MS)对胆汁、血浆和尿液进行分析, 鉴定入血成分和代谢产物。根据对这些成分质谱裂解规律的分析, 共鉴定了36种不同的黄酮类化合物, 其中包括13个新的代谢产物。本实验在体外对黄芩提取物进行分析, 鉴定了其中16个黄酮类成分, 大鼠胆汁中鉴定了25个黄酮类化合物, 血浆中15个黄酮类化合物, 尿液中14个黄酮类化合物。结果表明, 黄芩提取物中的黄酮类化合物以葡萄糖醛酸化、硫酸化和甲基化的为主要代谢途径。本实验首次对黄芩提取物的代谢产物进行综合分析。

关键词: 黄芩, 黄酮, 代谢物, UFLC-IT-TOF/MS

Abstract:

Flavonoids are the main bioactive components of Radix Scutellariae, a well-known traditional Chinese medicine. After oral administration of Radix Scutellariae extract (RSE), the bile, plasma and urine of rats were analyzed to identify the absorbed parent components and metabolites by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry (UFLC-IT-TOF/MS). A total of 36 different flavonoids, including 13 new metabolites, were identified or tentatively characterized in bile, plasma and urine of rats based on their fragmentation patterns. Among them, 16 flavonoids were identified in RSE in vitro, 25 flavonoids in rat bile, 15 flavonoids in rat plasma, and 14 flavonoids in rat urine. The results indicated that glucuronidation, sulfation and methylation were the major metabolic pathways of flavonoids. It is the first time that the metabolism of RSE was studied comprehensively.

Key words: Radix Scutellariae, Flavonoids, Metabolites, UFLC-IT-TOF/MS

中图分类号:

R917

Supporting:

Foundation item: National Key Special Project of Science and Technology for Innovation Drugs of China (Grant No. 2013ZX094-02202).
^*Correspondence author. Tel.: 86-25-83271289; Fax: 86-22-86342841

陈正为, 佟玲, 李淑明, 李东翔, 刘小琳, 丁黎^*, 朱永宏, 周水平, 孙鹤. 采用UFLC-IT-TOF/MS鉴定大鼠口服黄芩提取物后胆汁、血浆和尿液中的主要成分[J]. , DOI: 10.5246/jcps.2013.04.046.

Zhengwei Chen, Ling Tong, Shuming Li, Dongxiang Li, Xiaolin Liu, Li Ding^*, Yonghong Zhu, Shuiping Zhou, He Sun. Identification of major parent compounds and metabolites in bile, plasma and urine of rats after oral administration of Radix Scutellariae extract by UFLC-IT-TOF/MS[J]. , DOI: 10.5246/jcps.2013.04.046.

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