RP-HPLC法同时测定何首乌中8种活性成分的含量

RP-HPLC法同时测定何首乌中8种活性成分的含量

续艳丽, 董琦, 胡凤祖^*

1. 中国科学院西北高原生物研究所, 青海西宁 810008
2. 中国科学院研究生院, 北京 100049

收稿日期:2009-06-29 修回日期:2009-10-15 出版日期:2009-11-30 发布日期:2009-11-30
通讯作者: 胡凤祖*

Simultaneous quantitative determination of eight active components in
Polygonum multiflorum Thunb by RP-HPLC

Yan-Li Xu, Qi Dong, Feng-Zu Hu^*

1. Northwest Institute of Plateau Biology, CAS, Xining 810008, China
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received:2009-06-29 Revised:2009-10-15 Online:2009-11-30 Published:2009-11-30
Contact: Feng-Zu Hu*

摘要/Abstract

摘要：

建立RP-HPLC法同时测定何首乌中8种活性成分的方法, 其中2种二苯乙烯类(白藜芦醇苷、白藜芦醇)和6种黄酮类(芦丁、木樨草素、槲皮素、槲皮苷、山奈酚和异红草素)。采用kromasil C18柱(250 mm×4.6 mm, 5 μm), 甲醇(A): 水 (B)为流动相梯度洗脱。流速1 mL/min, 二苯乙烯类和黄酮类分别在320、350 nm波长下检测。结果2种二苯乙烯类和6种黄酮类与色谱峰面积均呈良好的线形关系, r>0.9995,平均回收率为96.8%-102.5%, RSD均小于4.8%。结果证明本方法可用于何首乌活性成分的含量测定, 也可用于何首乌药材的质量控制。

关键词: RP-HPLC, 二苯乙烯类, 黄酮类, 何首乌

Abstract:

A sensitive and specific reversed-phase high performance liquid chromatography (RP-HPLC) method was established for the simultaneous quantitative determination of eight active components, two stilbenes (resveratrol, polydatin) and six flavonoids (rutin, quercitrin, quercetin, luteotin, isoorientin, kaempferol), in Polygonum multiflorum Thunb. The chromatographic separation was performed on a Kromasil C18 column (250 mm×4.6 mm, 5 μm), and gradient elution was carried out with water-methanol as the mobile phase at a flow rate of 1 mL/min. Stilbenes and flavonoids were respectively detected at 320 nm and 350 nm with DAD. The correlation coefficients of all the calibration curves were found to be higher than 0.9995. The average recoveries were ranged from 96.8% to 102.5% with RSD less than 4.8% for these components. RP-HPLC was validated to be a robust method for the quantitative determination of active components in Polygonum multiflorum Thunb, and it could be used in the quality control of this traditional medicine.

Key words: RP-HPLC, RP-HPLC, Stilbenes, Stilbenes, Flavonoids, Flavonoids, Polygonum multiflorum Thunb, Polygonum multiflorum Thunb

中图分类号:

R917.01

Supporting:

^*Corresponding author. Tel.: 86-971-6132750; e-mail: hufz@nwipb.ac.cn

续艳丽, 董琦, 胡凤祖^*. RP-HPLC法同时测定何首乌中8种活性成分的含量[J]. .

Yan-Li Xu, Qi Dong, Feng-Zu Hu^*. Simultaneous quantitative determination of eight active components in
Polygonum multiflorum Thunb by RP-HPLC [J]. .

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