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Sensitive method for rapid estimation of Lornoxicam in bulk and its dosage form by RP-HPLC

Prajapati Prajesh*, Vaghela Vipul, Baraiya Dharmendra   

  1. 1. Institute of Research and Development, Gujarat Forensic Sciences University, Gandhinagar 382007, Gujarat, India.
    2. A.R. College of Pharmacy and G.H.Patel Institute of Pharmacy, Vallabh Vidyanagar 388120, Gujarat, India.
  • 收稿日期:2012-10-05 修回日期:2012-12-25 出版日期:2013-01-20 发布日期:2013-01-20
  • 通讯作者: Prajapati Prajesh*

Sensitive method for rapid estimation of Lornoxicam in bulk and its dosage form by RP-HPLC

Prajapati Prajesh*, Vaghela Vipul, Baraiya Dharmendra   

  1. 1. Institute of Research and Development, Gujarat Forensic Sciences University, Gandhinagar 382007, Gujarat, India.
    2. A.R. College of Pharmacy and G.H.Patel Institute of Pharmacy, Vallabh Vidyanagar 388120, Gujarat, India.
  • Received:2012-10-05 Revised:2012-12-25 Online:2013-01-20 Published:2013-01-20
  • Contact: Prajapati Prajesh*

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摘要：

Lornoxicam (6-chloro-4-hydroxy-2-methyl-N-2-pyridyl-2H-thieno-[2,3-e]-1,2-thiazine-3-carboxamide-1,1-dioxide) is persist as a non-steroidal anti-inflammatory drug of the oxicam class with analgesic, anti-inflammatory and antipyretic properties. A fast, accurate and sensitive chromatographic method for estimation of Lornoxicam was developed as no official method available for detection. The chromatographic separation employs isocratic elution by utilizing an inertsil ODS-C18, 250 mm×4.6 mm, 5 µm columns. Mobile phase consisting of solvent (40 mL acetonitrile and 60 mL 0.1 M phosphate buffer (pH 6.8 was adjusted with triethylamine)) endowed at a flow rate of 1.0 mL/min. The analyte was detected and quantified at 290 nm using UV detector. The method was validated according to ICH guidelines, illustrating to be accurate (recovery 99.08%-101.13%) and precise (intraday (0.27-1.32) and interday (0.59-1.59))within the corresponding linear range (10-60 µg/mL) with r2 0.9992.

关键词: Lornoxicam, RP-HPLC, Analgesic, Anti-inflammatory

Abstract:

Lornoxicam (6-chloro-4-hydroxy-2-methyl-N-2-pyridyl-2H-thieno-[2,3-e]-1,2-thiazine-3-carboxamide-1,1-dioxide) is persist as a non-steroidal anti-inflammatory drug of the oxicam class with analgesic, anti-inflammatory and antipyretic properties. A fast, accurate and sensitive chromatographic method for estimation of Lornoxicam was developed as no official method available for detection. The chromatographic separation employs isocratic elution by utilizing an inertsil ODS-C18, 250 mm×4.6 mm, 5 µm columns. Mobile phase consisting of solvent (40 mL acetonitrile and 60 mL 0.1 M phosphate buffer (pH 6.8 was adjusted with triethylamine)) endowed at a flow rate of 1.0 mL/min. The analyte was detected and quantified at 290 nm using UV detector. The method was validated according to ICH guidelines, illustrating to be accurate (recovery 99.08%-101.13%) and precise (intraday (0.27-1.32) and interday (0.59-1.59))within the corresponding linear range (10-60 µg/mL) with r2 0.9992.

Key words: Lornoxicam, RP-HPLC, Analgesic, Anti-inflammatory

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*Corresponding author. Tel.: +919825318996; Fax: 07923256251

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