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藏药榜嘎挥发油成分和抑菌活性研究

张春江1, 刘春梅2, 贠田1, 李红玉1*   

  1. 1. 兰州大学 生命科学学院, 甘肃 兰州 730000
    2. 兰州大学 化学化工学院, 甘肃 兰州 730000
  • 收稿日期:2009-04-06 修回日期:2009-08-10 出版日期:2009-09-15 发布日期:2009-09-15
  • 通讯作者: 李红玉*

Chemical composition and antimicrobial activities of the essential oil of Aconitum tanguticum

Chun-Jiang Zhang1, Chun-Mei Liu2, Tian Yun1, Hong-Yu Li1*   

  1. 1. College of Life Sciences, Lanzhou University, Lanzhou 73000, China
    2. College of Chemistry & Chemical Engineering, Lanzhou University, Lanzhou 730000, China
  • Received:2009-04-06 Revised:2009-08-10 Online:2009-09-15 Published:2009-09-15
  • Contact: Hong-Yu Li*

摘要:

对采集于甘肃甘南的藏药榜嘎挥发油的化学成分及抑菌活性进行了研究。采用水蒸气蒸馏法从榜嘎中提取挥发油, 并用GC、GC-MS法测定挥发油所含化学成分及各化合物在挥发油中的相对百分含量。鉴定出榜嘎挥发油的35个化学成分, 其中单萜类占41.7%, 倍半萜类占12.7%。挥发油中主要的化合物为: (-)-tran-pinecarvyl acetate (15.6%), 正庚烷(14.88%), 桉树脑 (6.82%), 3-蒎烷酮(3.64%), 松崁烷 (3.34%), 杜松醇 (2.93%), cubenol (2.33%)。并采用琼脂扩散法和肉汤稀释法评价挥发油对9种细菌, 1种酵母, 3种真菌的抑制作用。结果表明榜嘎挥发油对半裸镰刀菌、耐甲氧西林金黄色葡萄球菌具有强烈的抑制作用, 对大肠杆菌抑制作用很弱, 对絮状表皮癣菌没有抑制作用。最小抑菌浓度MIC的范围为0.125 到 5.000 mg/mL。本研究首次报道了藏药榜嘎挥发油的化学成分及抑菌活性。挥发油中鉴定的35种化合物均为榜嘎中发现的新成分。

关键词: 榜嘎挥发油, 气相色谱-质谱联用, 化学成分, 抑菌活性

Abstract: The composition and antimicrobial activities of the essential oil of Aconitum tanguticum from the southern part of Gansu Province of China were investigated. The essential oil of aerial parts was obtained by hydrodistillation with a 0.7% (v/w) yield. The chemical composition of the essential oil was analyzed by gas chromatography-mass spectrometry (GC-MS) method. Thirty-five compounds were characterized in the oil, which were mainly monoterpenoids (41.7%) and sequiterpenoids (12.7%). The main compounds in the oil were (-)-tran-pinecarvyl acetate (15.6%), heptane (14.88%), cineole (6.82%), 3-pinanone (3.64%), pinocamphone (3.34%), cadinol (2.93%), and cubenol (2.33%). These compounds are identified for the first time in Aconitum tanguticum. The antimicrobial activities of the oil were evaluated against 9 bacteria strains, 1 yeast strain and 3 fungi strains using agar diffusion and broth microdilution methods. It showed strong inhibition against Fusarium semitectum and methicillin-resistant Staphlococcus aureus, low activity against Escherichia coli, but no effect against Epidermophyton floccosum. Its MIC values ranged from 0.125-5.000 mg/mL. Thirty-five compounds are newly identified in Aconitum tanguticum.

Key words: Aconitum tanguticum, Aconitum tanguticum, Tibetan medicine, Tibetan medicine, Essential oil, Essential oil, Chemical composition, Chemical composition, Antimicrobial, Antimicrobial

中图分类号: 

Supporting: Foundation item: National Natural Science Foundation of China (Grant No. 30471133).
*Corresponding author. Tel.: 86-931-8915686; fax: 86-931-8915685; e-mail: lihy@lzu.edu.cn